Dulbecco modified eagle medium (dmem)

HEK293T (RRID: CVCL_0063), HEK293FT (RRID: CVCL_6911), DU145 (RRID: CVCL_0105), HeLa (RRID:CVCL_0030), H1299 (RRID:CVCL_B7N7), and mouse embryonic fibroblasts (MEFs) were cultured in DMEM supplemented with 10% FBS and 1% 100 U of penicillin, and 100 μg·mL⁻¹ streptomycin (Yeasen, Shanghai, China). PC3 (RRID:CVCL_0035) was cultured in RPMI1640 supplemented with 10% FBS and 1% 100 U of penicillin, and 100 μg·mL⁻¹ streptomycin (Yeasen). DU145‐PTEN^−/− was generated with CRSPR/Cas9. U2OS‐DR‐GFP was a gift from Dr Daming Gao [34 (link)]. Pten^WT and Pten^K254R MEFs were obtained from 13‐day pregnant mouse embryos of wild‐type and Pten^K254R knock‐in C57BL/6 mice, respectively, and then immortalized with SV40‐LT at passage three. The mice were purchased from BRL Medicine Company (Shanghai, China). The other cell lines were from Cell Bank/Stem Cell Bank, Chinese Academy of Sciences. All cell lines have been authenticated in the past 3 years by Short Tandem Repeat (STR) analysis. Experiments were performed in mycoplasma‐free cells. Plasmids and siRNA transfection were carried out with PEI for HEK293T, HEK293T^Senp1−/− and HEK293FT and Lipo2000 (Invitrogen, Carlsbad, CA, USA) for other cells following the manufacturer's protocol. Packaging lentiviral and subsequent infection of all cell lines were carried out according to protocol in our laboratory.