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Molecular imaging software standard edition v 5

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Molecular Imaging Software-Standard Edition-v.5.4.2.18893 is a software solution developed by Carestream for processing and analyzing molecular imaging data. It provides core functionalities for image acquisition, visualization, and quantification without any interpretation or extrapolation on intended use.

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Lab products found in correlation

Mouse anti pcsk9, by Cayman Chemical (4 mentions) Image station 4000r pro imaging system, by Carestream (4 mentions) Hrp labeled anti mouse secondary antibody, by Thermo Fisher Scientific (2 mentions) Avidin hrp, by Thermo Fisher Scientific (2 mentions) Supersignal west pico chemiluminescence substrate, by Thermo Fisher Scientific (2 mentions) Biotinylated sheep anti pcsk9 antibody, by R&D Systems (2 mentions) Casein, by Merck Group (2 mentions) Plasminogen, by Merck Group (1 mentions)

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Carestream Molecular Imaging Software (42 citations)

6 protocols using molecular imaging software standard edition v 5

1

Quantification of PCSK9 Protein Expression

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This technique was performed mostly as previously described [25 (link)]. Blocking with 2% BSA-TBS was performed for 30 minutes at room temperature. Incubation with the primary antibody mouse anti-PCSK9 (Cayman Chemicals; Ann Arbor, MI; diluted 1:1000) was performed overnight at 4°C. After incubating with the HRP-labeled anti-mouse secondary antibody (Fisher Scientific; diluted 1:2,000) or avidin-HRP (Fisher Scientific; diluted 1:500) for 1 hour at room temperature, the SuperSignal West Pico Chemiluminescence Substrate (Pierce ThermoFisher) was used for detection. Several exposures ranging from 0.5 s to 30 minutes were made using a Kodak Image Station 4000R Pro Imaging System (Bend, OR) and the Carestream Molecular Imaging Software-Standard Edition-v.5.4.2.18893 (New Haven, CT).
Wooten C.J., Krishnaji S.T., Melendez Q.M, & Lopez D. (2019). Identification of Proteins Interacting with PCSK9 Using a Protoarray Human Protein Microarray. International journal of biomedical investigation, 2(2), 120.
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2

Western Blot Immunodetection of Proteins

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This technique was performed essentially as previously described [21 ]. Blocking with 2% BSA-TBS or 5% non-fat dry milk-TTBS (depending on whether the antibody was biotinylated or not, respectively) was performed for 30 minutes at room temperature. Primary antibodies used were mouse anti-PCSK9 (Cayman; diluted 1:1000), rabbit anti-annexin A2 (diluted 1:250), mouse anti-A1AT (diluted 1 μg/mL), goat anti-APOH (diluted 1 μg/mL), goat anti-actin (used as internal control in some studies; diluted 1:250), and biotinylated sheep anti-PCSK9 antibody (R&D Systems; diluted 1:150). After incubating with HRP-labeled secondary antibodies (diluted 1:2,000) or avidin-HRP (diluted 1:500), depending on the primary antibody, immunoreactive proteins were detected using the SuperSignal West Pico Chemiluminescence Substrate. Several exposures ranging from 0.5 s to 30 minutes were made using a Kodak Image Station 4000R Pro Imaging System (Bend, OR) and the Carestream Molecular Imaging Software-Standard Edition-v.5.4.2.18893 (New Haven, CT). When needed, quantitation of the Western blot signals was performed using the Imaging software.
Melendez Q.M., Wooten C.J., Krishnaji S.T., Knagge K., Kirchner D, & Lopez D. (2020). Identification of Novel Proteins Interacting with Proprotein Convertase Subtilisin/Kexin 9. International journal of biomedical investigation, 3(1), 123.
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3

Quantification of PCSK9 Protein Expression

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This technique was performed mostly as previously described [25 (link)]. Blocking with 2% BSA-TBS was performed for 30 minutes at room temperature. Incubation with the primary antibody mouse anti-PCSK9 (Cayman Chemicals; Ann Arbor, MI; diluted 1:1000) was performed overnight at 4°C. After incubating with the HRP-labeled anti-mouse secondary antibody (Fisher Scientific; diluted 1:2,000) or avidin-HRP (Fisher Scientific; diluted 1:500) for 1 hour at room temperature, the SuperSignal West Pico Chemiluminescence Substrate (Pierce ThermoFisher) was used for detection. Several exposures ranging from 0.5 s to 30 minutes were made using a Kodak Image Station 4000R Pro Imaging System (Bend, OR) and the Carestream Molecular Imaging Software-Standard Edition-v.5.4.2.18893 (New Haven, CT).
Wooten C.J., Krishnaji S.T., Melendez Q.M, & Lopez D. (2019). Identification of Proteins Interacting with PCSK9 Using a Protoarray Human Protein Microarray. International journal of biomedical investigation, 2(2), 120.
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4

Western Blot Immunodetection of Proteins

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This technique was performed essentially as previously described [21 ]. Blocking with 2% BSA-TBS or 5% non-fat dry milk-TTBS (depending on whether the antibody was biotinylated or not, respectively) was performed for 30 minutes at room temperature. Primary antibodies used were mouse anti-PCSK9 (Cayman; diluted 1:1000), rabbit anti-annexin A2 (diluted 1:250), mouse anti-A1AT (diluted 1 μg/mL), goat anti-APOH (diluted 1 μg/mL), goat anti-actin (used as internal control in some studies; diluted 1:250), and biotinylated sheep anti-PCSK9 antibody (R&D Systems; diluted 1:150). After incubating with HRP-labeled secondary antibodies (diluted 1:2,000) or avidin-HRP (diluted 1:500), depending on the primary antibody, immunoreactive proteins were detected using the SuperSignal West Pico Chemiluminescence Substrate. Several exposures ranging from 0.5 s to 30 minutes were made using a Kodak Image Station 4000R Pro Imaging System (Bend, OR) and the Carestream Molecular Imaging Software-Standard Edition-v.5.4.2.18893 (New Haven, CT). When needed, quantitation of the Western blot signals was performed using the Imaging software.
Melendez Q.M., Wooten C.J., Krishnaji S.T., Knagge K., Kirchner D, & Lopez D. (2020). Identification of Novel Proteins Interacting with Proprotein Convertase Subtilisin/Kexin 9. International journal of biomedical investigation, 3(1), 123.
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5

Casein Zymography for PAI-1 Activity

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Thirty micrograms of total cell lysate from PAI-1 clones (T24Scr, T24-PAI-1KD, UM-UC-14Scr, UM-UC-14-PAI-1KD, HeLaEmpty, HeLa-PAI-1OE) and parental tumor cells (T24, UM-UC-14, and HeLa treated in the presence or absence of tiplaxtinin) were electrophoresed on 10% SDS-polyacrylamide gels containing 1 mg/ml casein (Sigma, St. Louis, MO), 10 μg/ml plasminogen (Sigma, St. Louis, MO) and 0.5 mU/ml uPA under non-reducing conditions. After electrophoresis, SDS removal and PAI-1 renaturation were achieved by washing the gel for 1 hr in buffer containing 2.5% Triton-X 100, 50 mM tris pH 7.4, 5 mM CaCl2, and 1 μM ZnCl2. The gel was then incubated in a reaction buffer containing 50 mM Tris pH 7.4, 5 mM CaCl2, 1 μM ZnCl2 and 0.02% NaN3 pH 8.0) for 18 hr at 37°C, followed by staining with Coomassie blue. PAI-1 activity was indicated as lytic zones of plasmin generation. Gel was photographed using the KODAK Gel Logic 200 Imaging System with Carestream Molecular Imaging Software Standard Edition v5.0.7.24 (Carestream Health, Rochester, NY) when opaque bands appeared on a clear background.
Giacoia E.G., Miyake M., Lawton A., Goodison S, & Rosser C.J. (2014). PAI-1 Leads to G1-phase Cell Cycle Progression through Cyclin D3/CDK4/6 Up-regulation. Molecular cancer research : MCR, 12(3), 322-334.
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6

Evaluating MMP-10 Activity in Cancer Cell Lines

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Thirty micrograms of total cell lysate from the parental cervical cancer cell line HeLa and parental urothelial cell line UROtsa as well as 30 μg of total cell lysate from the following clones: HeLa-MMP-10OE, HeLaEmpty, UROtsa-MMP-10KD1&2 and UROtsa-MMP-10Scr, were electrophoresed on 10% SDS-polyacrylamide gels containing 1 mg/ml casein (Sigma, St. Louis, MO) under non-reducing conditions. After electrophoresis and SDS removal, MMP-10 renaturation was achieved by washing the gel for 1 hr in buffer containing 2.5% Triton-X 100, 50 mM tris pH 7.4, 5 mM CaCl2, and 1 μM ZnCl2. Gels were then incubated in a reaction buffer containing 50 mM Tris pH 7.4, 5 mM CaCl2, 1 μM ZnCl2 and 0.02% NaN3 pH 8.0 for 18 hrs at 37°C, followed by staining with Coomassie blue. MMP-10 activity was indicated by the degradation of casein. Gels were photographed using the KODAK Gel Logic 200 Imaging System with Carestream Molecular Imaging Software Standard Edition v5.0.7.24 (Carestream Health, Rochester, NY).
Zhang G., Miyake M., Lawton A., Goodison S, & Rosser C.J. (2014). Matrix metalloproteinase-10 promotes tumor progression through regulation of angiogenic and apoptotic pathways in cervical tumors. BMC Cancer, 14, 310.
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