Image pro plus 4

Manufactured by Media Cybernetics

Sourced in United States, Japan, Germany, Italy, China, Belgium

Image-Pro Plus 4.5 is a comprehensive image analysis software that provides a wide range of tools for processing, measuring, and analyzing digital images. The software supports a variety of image file formats and is designed to work with a wide range of imaging equipment and devices.

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Lab products found in correlation

Bx50 microscope, by Olympus (27 mentions) Th4 200, by Olympus (5 mentions) Crystal violet, by Merck Group (4 mentions) Low melting point agarose, by Duchefa Biochemie (4 mentions) Bca protein assay, by Thermo Fisher Scientific (4 mentions) Bx50 epifluorescence microscope, by Olympus (4 mentions) Axioplan 2 microscope, by Zeiss (4 mentions) Eclipse te2000 u fluorescence microscope, by Nikon (4 mentions) Mouse anti p p53 ser15, by Cell Signaling Technology (4 mentions) Rabbit anti p erk thr202 tyr204, by Cell Signaling Technology (4 mentions) Pvdf membrane, by Roche (4 mentions) Lsm 510, by Zeiss (4 mentions) Axioplan microscope, by Zeiss (4 mentions) Cell lysis buffer, by Cell Signaling Technology (3 mentions) Odyssey infrared imaging system, by LI COR (3 mentions) Hematoxylin, by Merck Group (3 mentions) Q color 3, by Olympus (3 mentions) β actin, by Abcam (3 mentions) Bx50 light microscope, by Olympus (3 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (3 mentions)

Spelling variants of this product

Image-Pro Plus (1608 citations) Image-Pro (129 citations) Image Pro Plus software version 4.5 (30 citations) Image-Pro Plus software (v.4) (10 citations) Image Pro 4.5 (9 citations) Image-Pro Plus Software (vs.4) (3 citations) Image-Pro® plus 4.5 image analysis software (3 citations) Image-Pro Plus version 4.5 for Windows (2 citations) Image-Pro Plus 4.5 for Windows (2 citations) Image-Pro Plus 4.5 System (2 citations)

335 protocols using image pro plus 4

Quantitative Histological Analysis of Kidney Lipids and Glomerular Morphology

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The left kidney was used for glomerular injury analysis in H&E stained (Sigma) sections of the kidney (5 μm) embedded in Paraplast. Digital images from thirty glomeruli per animal were obtained using a light microscope (Leica) at 400x magnification. After digitalization, Bowman's capsule area (BCA), glomerular tuft area (GTA), and Bowman's space area (BSA) were traced and calculated using a computerized morphometric analysis system (Image Pro-Plus 4.1; Media Cybernetics, Silver Spring, MD, USA).
Lipid content was measured using quantitative histochemistry of Oil Red O (Sigma-Aldrich) stained kidneys. Tissue sections (8 μm thickness) obtained in a cryostat were examined by light microscopy at 200x magnification and analyzed by a computerized morphometric analysis system (Image Pro-Plus 4.1; Media Cybernetics, Silver Spring, MD, USA). The slides were counterstained with hematoxylin to visualize the nuclei. Lipid accumulation was determined in 12 images per animal based on the percentage of area occupied by lipid droplets. Histological analyses were blinded conducted by RO Pereira.

Muller C.R., Leite A.P., Yokota R., Pereira R.O., Americo A.L., Nascimento N.R., Evangelista F.S., Farah V., Fonteles M.C, & Fiorino P. (2019). Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats. Frontiers in Nutrition, 6, 60.

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Quantitative Lung Histopathology Analysis

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Lung sections were stained with H&E and images were taken using an Olympus BX40 microscope (Olympus America, Melville, NY) at 20X with Image Pro Plus 4.0 software (Media Cybernetics, Silver Spring, MD). 4 images per animal were taken and analyzed in a blinded fashion. Alveolar counts and septal thickness were measured using research-based digital image analysis software (Image Pro Plus 4.0; Media Cybernetics, Silver Spring, MD) [21 (link)].

Perez M., Lee K.J., Cardona H.J., Taylor J.M., Robbins M.E., Waypa G.B., Berkelhamer S.K, & Farrow K.N. (2017). Aberrant cGMP signaling persists during recovery in mice with oxygen-induced pulmonary hypertension. PLoS ONE, 12(8), e0180957.

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Histological Analysis of Organ Pathology

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Mice were anesthetized and spleen, livers and kidneys were isolated. Each organ was fixed with 10% paraformaldehyde in PBS and dehydrated through graded ethanol washes, and embedded in paraffin. 5 μm sections were collected on slides and stained with hematoxylin and eosin (H&E). The semi-quantitative analysis of histological changes was conducted as previously described using a computerized program30 (link)64 (link). 10 digital images were taken from each H&E stained mouse tissue section at 20X magnification from different areas. The congestion, necrosis or cysts on sections were identified according to their structure and color. Briefly, the dark red color was chosen for quantification of congestion and it was performed on 10 fields/mouse tissue sections at 20X magnification using software analysis (Image Pro Plus 4.0; Media Cybernetics, Bethesda, MD, USA) Additionally, the areas of necrosis in the livers and congestion in the spleen and lung were first manually marked by using a magical pen tool available in Adobe Photoshop Program. Then the quantification was conducted on 10 fields/mouse tissue sections at 20x using software analysis (Image Pro Plus 4.0; Media Cybernetics, Bethesda, MD, USA). The whole areas of each image were considered as 100%. The percentage of pathological areas to whole area of image was recorded.

Wu H., Bogdanov M., Zhang Y., Sun K., Zhao S., Song A., Luo R., Parchim N.F., Liu H., Huang A., Adebiyi M.G., Jin J., Alexander D.C., Milburn M.V., Idowu M., Juneja H.S., Kellems R.E., Dowhan W, & Xia Y. (2016). Hypoxia-mediated impaired erythrocyte Lands’ Cycle is pathogenic for sickle cell disease. Scientific Reports, 6, 29637.

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Quantitative Histological Analysis of Mouse Tissues

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Mice were anesthetized and organs were isolated and fixed with 10% paraformaldehyde in PBS overnight at 4 °C. Fixed tissues were rinsed in PBS, dehydrated through graded ethanol washes, and embedded in paraffin. 5μm sections were collected on slides and stained with hematoxylin and eosin (H&E). The semi-quantitative analysis of histological changes was conducted as previously described using a computerized program^{7 (link)}. Ten digital images were taken from each H&E stained mouse tissue section at 20X magnification from different areas. The congestion, necrosis or cysts on sections were identified according to their structure and color. Briefly, the dark red color was chosen for quantification of congestion and it was performed on 10 fields/mouse tissue sections at 20× magnification using software analysis (Image Pro Plus 4.0; Media Cybernetics, Bethesda, MD, USA). Additionally, the areas of necrosis in the livers and cysts in the renal cortex were first manually marked by a magical pen tool available in Adobe Photoshop Program. Then the quantification was conducted on 10 fields/mouse tissue sections at 20× using software analysis (Image Pro Plus 4.0; Media Cybernetics, Bethesda, MD, USA). The whole areas of each image were considered as 100%. The percentage of pathological areas to whole area of image was recorded.

Sun K., D’Alessandro A., Ahmed M.H., Zhang Y., Song A., Ko T.P., Nemkov T., Reisz J.A., Wu H., Adebiyi M., Peng Z., Gong J., Liu H., Huang A., Wen Y.E., Wen A.Q., Berka V., Bogdanov M.V., Abdulmalik O., Han L., Tsai A.L., Idowu M., Juneja H.S., Kellems R.E., Dowhan W., Hansen K.C., Safo M.K, & Xia Y. (2017). Structural and Functional Insight of Sphingosine 1-Phosphate-Mediated Pathogenic Metabolic Reprogramming in Sickle Cell Disease. Scientific Reports, 7, 15281.

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Microvessel Density Quantification Protocol

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The tumor MVD was calculated by the method presented by Weidner with certain modifications (25 (link)). First, five fields with the highest blood vessels densities were identified at ×100 magnification under a light microscope; microvessels were then counted on the monitor of a computer image analysis system (Image Pro Plus 4; Media Cybernetics, Rockville, MD, USA) under a light microscope at ×200 magnification, and the mean microvessel number was calculated as the MVD value. No matter whether a tube was formed, any single EC or multiple ECs arranged compactly were assumed to be a blood vessel. When a tubular structure was not continuous phase, its branches were also viewed as a blood vessels. Cells that were difficult to distinguish or dimly stained, as well as thick-walled muscular vessels or lumen of >50 µm (equivalent to ≥1 cm at magnification, ×200) were not counted.

Li B., Nie Z., Zhang D., Wu J., Peng B., Guo X., Shi Y., Cai X., Xu L, & Cao F. (2017). Roles of circulating endothelial progenitor cells and endothelial cells in gastric carcinoma. Oncology Letters, 15(1), 324-330.

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Confocal Microscopy for Live Cell Imaging

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All confocal images (except FRET, which is described below) were acquired with a microscope (TCS SP5; Leica) equipped with the software LAS AF 1.82 and following lasers: optically pumped semiconductor laser 488 nm/270 mW, diode-pumped solid-state laser 561 nm/20 mW, and HeNe 633 nm/12 mW. For fixed samples, the glycerol immersion objective HCX Plan Apochromat 63×/1.3 NA GLYC CORR CS (glycerol correction; Leica) at RT was used. Live cell microscopy (FRAP and Motility analysis, see below) was carried with HCX Apochromat L 63×/0.90 NA water immersion objective. Neurons were maintained at 37°C and 5% CO₂ in a custom chamber (The Box; Life Imaging Services) in Neurobasal medium containing B27 supplement and 0.5 mM l-glutamine throughout the imaging session.
For quantification of phospho-cofilin in Fig. 2 b, fluorescent wide-field images were acquired with microscope (AxioImager.M1; Carl Zeiss) equipped with camera (AxioCam HR; Carl Zeiss) and AxioVision 4 software (Carl Zeiss). The hippocampal neurons were imaged with 40× objective EC Plan Neofluar/0.75/Ph2 at RT. The light-emitting diode system (Colibri; Carl Zeiss) with 470- and 555-nm modules was used, and the beam combiners were BC490, BC425 (for GFP), and BC565 (for Alexa Flour 568). The mean fluorescent density was measured in Image-Pro Plus 4 (Media Cybernetics) after subtraction of the background.

Llano O., Smirnov S., Soni S., Golubtsov A., Guillemin I., Hotulainen P., Medina I., Nothwang H.G., Rivera C, & Ludwig A. (2015). KCC2 regulates actin dynamics in dendritic spines via interaction with β-PIX. The Journal of Cell Biology, 209(5), 671-686.

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Histological Analysis of Intestinal Morphology

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Mice were sacrificed and jejunum samples were collected and fixed in 10% formalin with 2% sucrose in phosphate buffer for 4 h at 4°C and processed for paraffin embedding. Other jejunum samples were collected and preserved in liquid nitrogen for mRNA and protein extraction.
For histological examination, slides were stained with hematoxylin and eosin. Histological images were captured and digitized. Villus height was measured using Image Pro Plus 4 image analysis software (Media Cybernetics, Baltimore, MD). The degree of intestinal tissue injury was evaluated on a grading scale of : +++ = severe; ++ = mild; + = light; 0 = absent.

Cardani D., Sardi C., La Ferla B., D’Orazio G., Sommariva M., Marcucci F., Olivero D., Tagliabue E., Koepsell H., Nicotra F., Balsari A, & Rumio C. (2014). Sodium glucose cotransporter 1 ligand BLF501 as a novel tool for management of gastrointestinal mucositis. Molecular Cancer, 13, 23.

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Quantifying Actin Dynamics in TSPC Subtypes

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Quantification of Y- and A-TSPC (EphA4-Fc, EphB2-Fc, and Fc-control) cell area was obtained from F-actin images by measuring 50 cells from each donor with the polygonal tool of the ImageProPlus4 software (Media Cybernetics). Actin dynamics of Y- and A-TSPC (EphA4-Fc, EphB2-Fc, and Fc-control) was analyzed by latrunculin A experiment, carried out as described in (Kohler et al., 2013 (link)). latrunculin A inhibits actin polymerization by sequestering monomeric G-actin and thereby disrupts the turnover of actin filaments. In brief, the four different TSPC groups, Y- and A-TSPC with EphA4-Fc, EphB2-Fc, and Fc-control, (5.5 × 10³ cells/cm²) were grown in 96 well plates and pre-cultured for 48 h. Then, cells were treated with 0.4 μM latrunculin A (Sigma–Aldrich), fixed at different time intervals (0, 2, 5, 8, 10, 20, 30, and 60 min), stained with 0.6 μM phalloidin-546 (Thermo Scientific) and fluorescence signals were recorded at 573 nm using a SAFIRE2 microplate reader (Tecan, Germany). In each group, F-actin content at time point 0 was set to 100%. latrunculin A analyses were reproduced three independent times for all Y- and A-TSPC donors in triplicates (n = 9).

Popov C., Kohler J, & Docheva D. (2016). Activation of EphA4 and EphB2 Reverse Signaling Restores the Age-Associated Reduction of Self-Renewal, Migration, and Actin Turnover in Human Tendon Stem/Progenitor Cells. Frontiers in Aging Neuroscience, 7, 246.

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Rat Nerve Transposition and Stimulation

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The rats were fixed on a laboratory-made operating table after anesthesia. The affected limb was held firm with one tack in front of and one behind the elbow joint of the affected limb. An incision of the skin was made in the middle of the upper left arm of the rat to expose and then bluntly separate the nerve. In the sham operation group and epineurial neurorrhaphy group, the median nerves were directly stimulated. In the musculocutaneous nerve transposition, medial pectoral nerve transposition, and radial nerve muscular branch transposition groups, a site 5 mm from the sleeve suture was stimulated with a Synergy electrophysiologic apparatus (Medelec Synergy; Oxford Instrument Inc., United Kingdom) with 0.9 mA continuous square waves at 50 Hz. The position of each left front paw was recorded with a digital camera (Sony, Tokyo, Japan) before and after stimulation. Results were synthesized with Image Pro Plus 4.5 software (Media Cybernetics, Silver Spring, MD, USA), and the angle of wrist flexion was measured.

Yuan Y.S., Niu S.P., Yu Y.L., Zhang P.X., Yin X.F., Han N., Zhang Y.J., Zhang D.Y., Xu H.L., Kou Y.H, & Jiang B.G. (2019). Reinnervation of spinal cord anterior horn cells after median nerve repair using transposition with other nerves. Neural Regeneration Research, 14(4), 699-705.

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Immunohistochemical Analysis of Liver Tissues

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The liver tissues were fixed in formalin solution, dehydrated with gradually increasing concentrations of ethanol, embedded in paraffin, and sectioned. The 5 μm sections were blocked with a buffered blocking solution (3% bovine serum albumin in phosphate-buffered saline (PBS) for 15 min. Then, the sections were co-incubated with primary antibody for NF-kB, p38, p-p38 and Nrf2 at a dilution of 1:50 in PBS (v/v), at 4 °C overnight, followed by washing with PBS and co-incubating with secondary antibody at a dilution of 1:500 in PBS (v/v), at room temperature for 1 h. Thereafter, sections were washed with Tris-HCl 0.05 M, pH 7.66, and then co-incubated with a 3, 3′-diaminobenzidine solution in darkness, at room temperature for 10 min. The sections were washed with Tris-HCl, stained with hematoxylin according to standard protocols, and observed under a light microscope. The Image-ProPlus 4.5 Software (Media Cybernetics, Rockville, MD, USA) was used to analyze the expression of proteins.

Wang C., Huo X., Gao L., Sun G, & Cao L. (2017). Hepatoprotective Effect of Carboxymethyl Pachyman in Fluorouracil-Treated CT26-Bearing Mice. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(5), 756.

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