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Ti2 inverted microscope

Manufactured by Nikon
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Sourced in United States, Japan

The Ti2 inverted microscope is a versatile and advanced imaging system designed for a wide range of laboratory applications. It features a high-quality optical system, providing clear and detailed observation of samples. The Ti2 is capable of various observation techniques, including brightfield, phase contrast, and differential interference contrast (DIC) imaging. With its inverted design, the Ti2 microscope allows for easy access and manipulation of samples during experimentation.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (6 mentions) Csu w1 spinning disc system, by Yokogawa (5 mentions) Scmos camera, by Teledyne (5 mentions) Spectra x led light engine, by Lumencor (2 mentions) Pdgfr tyrosine kinase inhibitor 4, by Merck Group (2 mentions) Nsc23766, by Bio-Techne (2 mentions) Gsk269962, by Bio-Techne (2 mentions) Environmental chamber, by Nikon (2 mentions) Apo lambda 40x cf160 plan apochromat lambda 40x objective lens, by Oxford Instruments (2 mentions) Vsc 08688 camera, by Oxford Instruments (2 mentions) Lsrfortessa, by FlowJo (2 mentions) Cytof software v7, by Standard BioTools (2 mentions) Hyperion imaging system, by Standard BioTools (2 mentions) Pluronic f 127, by Merck Group (2 mentions) Scmos camera, by Hamamatsu Photonics (2 mentions) Picrosirius red, by Merck Group (1 mentions) Ds qi2, by Nikon (1 mentions) Masson s trichrome, by Merck Group (1 mentions) Ds fi3, by Nikon (1 mentions) Spectra x, by Lumencor (1 mentions)

Close spelling variants of this product

Inverted microscope (910 citations) Ti2 microscope (102 citations) Ti2-E inverted microscope (54 citations) Eclipse Ti2-E inverted microscope (33 citations) Eclipse Ti2-U inverted microscope (11 citations) Eclipse Ti2 Inverted Microscope System (7 citations) ECLIPSE Ti2-A inverted microscope (5 citations) Ti2-E inverted fluorescence microscope (5 citations) Eclipse Ti2 inverted fluorescence microscope (5 citations) Ti2 inverted fluorescence microscope (5 citations)

50 protocols using ti2 inverted microscope

1

Live-cell Imaging of Fibroblast Migration

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Nuclear labelled fibroblasts were seeded at approx. 7 x 103 cells per well in 24 well glass bottom MatTek dish and imaged approximately 8 hrs later. Bright-field and epifluorescence time-lapse imaging was performed at 37 °C and 5% CO2 with an inverted microscope (Nikon Ti2 inverted microscope fitted with a Okolab environmental chamber and CO2 mixer). Bright-field and epifluorescence images were taken every 10 min through a ×10 PlanFluor, NA 0.3 Ph1, Nikon objective. The imaging system includes a SpectraX LED light engine (Lumencor) fitted with standard filters and Photometrics Prime scientific CMOS camera. The microscope was managed using Micro-Manager v2.0 software.
Where indicated cell were pre-treated with PDGFR Tyrosine Kinase Inhibitor IV (Merck, 521233) at a final concentration of 500nM, NSC23766 (Tocris, 20161) at a final concentration of 20uM or GSK269962 (Tocris, 4009) at a final concentration of 20nM 2 hr prior to imaging. Images were acquired at 10 min intervals, and the cells tracked using the ImageJ Trackmate plug in. Persistence was calculated as the ratio of shortest linear distance between two points of migration (displacement) to the total distance traversed by the cell (distance) over 12 or 16 hr intervals.
Park D., Wershof E., Boeing S., Labernadie A., Jenkins R.P., George S., Trepat X., Bates P.A, & Sahai E. (2019). Extracellular matrix anisotropy is determined by TFAP2C-dependent regulation of cell collisions. Nature materials, 19(2), 227-238.
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2

Histological Analysis of Heart Tissue

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The protocol for histologic analyses has been described [6 (link)–10 (link)]. Briefly, all studies were performed on formalin-fixed, paraffin-embedded tissues. Four-μm-thick heart sections were subjected to Masson’s trichrome and picrosirius red staining according to the manufacturer’s recommendations (Sigma-Aldrich, St. Louis, MO). Immunohistochemistry staining was performed using specific antibodies against CD45 (Abcam, Cambridge, MA) with corresponding secondary antibodies and counterstained with DAPI (Thermo Fisher Scientific, Waltham, MA). Microscopic evaluation of labeled slides was performed using a NIKON Ti2 inverted microscope equipped with DS-Qi2 and DS-Fi3, and monochrome and color cameras, respectively.
Tang X.L., Wysoczynski M., Gumpert A.M., Li Y., Wu W.J., Li H., Stowers H, & Bolli R. (2021). Effect of intravenous cell therapy in rats with old myocardial infarction. Molecular and cellular biochemistry, 477(2), 431-444.
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3

Widefield Microscopy Imaging Protocol

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All widefield images were collected with a Nikon Ti-2 inverted microscope equipped with a 20×/0.75 or a 40×/0.95 (magnification/numerical aperture) objective and the Perfect Focus System for maintenance of focus over time. Fluorophores were excited with a Lumencor SpectraX light engine. AF-594 was excited with the 57- nm line from a 330 mW light-emitting diode source and collected with a DM593 dichroic mirror and a 624/40 nm emission filter. DAPI was excited with the 395-nm line from a 295 mW light-emitting diode source and collected with a DM409 dichroic mirror and a 447/60 nm emission filter. Images were acquired with a Nikon DS-Qi2 sCMOS camera controlled with NIS Elements AR software. Multiple stage positions were collected using a motorized piezo stage. Whole slide scanning was performed using NIS Elements JOBS to acquire 6 × 6 20× images to cover the whole sample circle. Frames were stitched using 5% overlap at the edges and automatic shading correction.
Fisher J., Mohanty T., Karlsson C.A., Khademi S.M., Malmström E., Frigyesi A., Nordenfelt P., Malmstrom J, & Linder A. (2021). Proteome Profiling of Recombinant DNase Therapy in Reducing NETs and Aiding Recovery in COVID-19 Patients. Molecular & Cellular Proteomics : MCP, 20, 100113.
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4

Immunofluorescence Staining Protocol

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Cells were fixed in 4% PFA for 15 min, and blocked using 0.5% BSA with 0.1% Triton-X100 in PBS (no Ca2+, no Mg2+) or 0.5% dry milk with 0.4% Triton-X100 in PBS (no Ca2+, no Mg2+) for 1 h at room temperature or overnight at 4°C. Then, cells were treated with primary antibody solution (see Supplementary Table S2) resuspended in 0.5% BSA with 0.1% Triton-X100 in PBS (no Ca2+, no Mg2+) or 0.5% dry milk with 0.4% Triton-X100 in PBS (no Ca2+, no Mg2+) and incubated overnight at 4°C. The following day, samples were washed with PBS (no Ca2+, no Mg2+), treated with secondary antibody solution at 1:1,000 (see Supplementary Table S2, and incubated for 1 h at room temperature or overnight at 4°C. Finally, samples were washed with PBS (no Ca2+, no Mg2+), counterstained with 5 μg/mL Hoechst, and imaged using a Nikon Ti-2 inverted microscope. Image analysis was performed using ImageJ Fiji software to automatically threshold and tabulate cell attributes.
Floy M.E., Shabnam F., Givens S.E., Patil V.A., Ding Y., Li G., Roy S., Raval A.N., Schmuck E.G., Masters K.S., Ogle B.M, & Palecek S.P. (2023). Identifying molecular and functional similarities and differences between human primary cardiac valve interstitial cells and ventricular fibroblasts. Frontiers in Bioengineering and Biotechnology, 11, 1102487.
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5

Live-cell Microscopy of Protein Dynamics

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Images were acquired using a Nikon Ti2 Inverted Microscope with Perfect Focus to correct for axial focal drift. A 60× oil objective with a .85 aperture from Nikon was used along with immersion oil from Nikon. The microscope is equipped with an environmental chamber that is heat-regulated and humidified and all images were taken at 37°C and 5% CO2 with varying concentrations of oxygen as noted in each experiment. All fluorescent images utilized fluorescently tagged proteins with GFP acquired using 488 nm laser excitation and ARP at 638 nm excitation using a SPECTRA X LED light engine. The camera used for image acquisition was CoolSNAP MYO and was run using the Nikon Elements Advanced Research Acquisition and Analysis Duo Package.
Jensen C.C., Clements A.N., Liou H., Ball L.E., Bethard J.R., Langlais P.R., Toth R.K., Chauhan S.S., Casillas A.L., Daulat S.R., Kraft A.S., Cress A.E., Miranti C.K., Mouneimne G., Rogers G.C, & Warfel N.A. (2023). PIM1 phosphorylates ABI2 to enhance actin dynamics and promote tumor invasion. The Journal of Cell Biology, 222(6), e202208136.
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6

Imaging LAMP1-labeled Organelles in Cells

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After fixation, cells were imaged using Nikon Ti2 inverted microscope. LAMP1 positive puncta and nuclear staining were imaged using the green channel (GFP) and blue channel (DAPI), respectively. GFP images were acquired at 25% LED intensity and 200 ms exposure. DAPI images were acquired at 15% LED intensity and 75 ms exposure settings. Images were acquired using CFI PLAN APO LAMBDA 40X CF160 Plan Apochromat Lambda 40X objective lens, N.A. 0.95, W.D. 0.17–0.25 mm, F.O.V. 25 mm, DIC, Correction collar 0.11–0.23 mm, Spring Loaded, and using Andor Zyla VSC-08688 camera.
Beesabathuni N.S., Park S, & Shah P.S. (2022). Quantitative and temporal measurement of dynamic autophagy rates. Autophagy, 19(4), 1164-1183.
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7

Printed Arrays Stability Test

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Baseline imaging of printed arrays was conducted at an exposure time of 1s using a Nikon Ti-2 inverted microscope. Images were captured at 4× and 10× magnifications. Stability testing involved imaging under the same parameters after 30 mins of immersed water wash at 220 RPM using a shaking incubator (VWR, Ontario, Canada). Food overlay imaging using romaine lettuce, ground beef, and whole chicken was done under the same parameters, but with 10 mm thick samples placed on the arrays.
Khan S., Shakeri A., Monteiro J.K., Tariq S., Prasad A., Gu J., Filipe C.D., Li Y, & Didar T.F. (2024). Comprehensive fluorescence profiles of contamination-prone foods applied to the design of microcontact-printed in situ functional oligonucleotide sensors. Scientific Reports, 14, 8277.
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8

Screening Neuron-Specific Protein Localization

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A strain expressing mNeonGreen::ESYT-2 in DA9 neuron (sybIs50) was mutagenized with EMS according to standard protocols (Anderson, 1995 (link)). 1000 F1s were cloned into different plates, and 30–50 F2 animals from individual F1 animals were subjected to a direct visual screen under a microscope. The mutants were isolated based on the changes in mNeonGreen::ESYT-2 distribution in DA9 neuron as observed with a 40x objective on either a Leica DMi8 microscope or a Nikon Ti2 inverted microscope.
Sun J., Harion R., Naito T, & Saheki Y. (2021). INPP5K and Atlastin-1 maintain the nonuniform distribution of ER–plasma membrane contacts in neurons. Life Science Alliance, 4(11), e202101092.
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9

Live-cell Imaging of Fibroblast Migration

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Nuclear labelled fibroblasts were seeded at approx. 7 x 103 cells per well in 24 well glass bottom MatTek dish and imaged approximately 8 hrs later. Bright-field and epifluorescence time-lapse imaging was performed at 37 °C and 5% CO2 with an inverted microscope (Nikon Ti2 inverted microscope fitted with a Okolab environmental chamber and CO2 mixer). Bright-field and epifluorescence images were taken every 10 min through a ×10 PlanFluor, NA 0.3 Ph1, Nikon objective. The imaging system includes a SpectraX LED light engine (Lumencor) fitted with standard filters and Photometrics Prime scientific CMOS camera. The microscope was managed using Micro-Manager v2.0 software.
Where indicated cell were pre-treated with PDGFR Tyrosine Kinase Inhibitor IV (Merck, 521233) at a final concentration of 500nM, NSC23766 (Tocris, 20161) at a final concentration of 20uM or GSK269962 (Tocris, 4009) at a final concentration of 20nM 2 hr prior to imaging. Images were acquired at 10 min intervals, and the cells tracked using the ImageJ Trackmate plug in. Persistence was calculated as the ratio of shortest linear distance between two points of migration (displacement) to the total distance traversed by the cell (distance) over 12 or 16 hr intervals.
Park D., Wershof E., Boeing S., Labernadie A., Jenkins R.P., George S., Trepat X., Bates P.A, & Sahai E. (2019). Extracellular matrix anisotropy is determined by TFAP2C-dependent regulation of cell collisions. Nature materials, 19(2), 227-238.
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10

Inkjet and Contact Printed Arrays for Biomolecule Detection

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Inkjet and contact printed arrays were incubated with 0.5 M sodium hydroxide (Millipore Sigma, Texas, United States) as a positive test condition and deionized water as a negative test condition21 (link). Arrays were imaged prior to incubation to establish baseline intensities. Arrays were then incubated with 200 μL of test solution for 2 h and subsequently imaged again. All imaging occurred at 1 s exposure using a Nikon Ti-2 inverted microscope. The visibility of positive state fNAPs when overlaid with food involved imaging with 10 mm samples of romaine lettuce, ground beef, and whole chicken placed on top of the arrays.
Khan S., Shakeri A., Monteiro J.K., Tariq S., Prasad A., Gu J., Filipe C.D., Li Y, & Didar T.F. (2024). Comprehensive fluorescence profiles of contamination-prone foods applied to the design of microcontact-printed in situ functional oligonucleotide sensors. Scientific Reports, 14, 8277.
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