Novaseq instrument s prime flowcell

Manufactured by Illumina

The NovaSeq S-Prime flow cell is a component of the NovaSeq 6000 Sequencing System, a high-throughput DNA sequencing instrument manufactured by Illumina. The S-Prime flow cell is designed to process samples and generate sequencing data. Its core function is to provide a platform for DNA template preparation, cluster generation, and sequencing reactions during the DNA sequencing workflow.

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Lab products found in correlation

Kapa hyper library preparation kit, by Roche (2 mentions)

Spelling variants of this product

NovaSeq (1417 citations) NovaSeq instrument (88 citations) NovaSeq S Prime (4 citations)

2 protocols using novaseq instrument s prime flowcell

Kapa Hyper Library Preparation for cfDNA Sequencing

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Libraries for next generation sequencing were prepared using the Kapa Hyper Library Preparation Kit (KAPA Biosystems) with 7.0–50.0 ng cfDNA (median 30.5 ng) or 50 ng for germline DNA as input. xGen CS-adapters – Tech Access (IDT-DNA) with Unique Molecular Identifiers (UMIs) on both strands and primers containing unique indexes on both strands were used to generate indexed libraries. Plasma libraries were pooled equimolarly and paired-end sequenced (2 × 151 bp) on an Illumina Novaseq instrument (S-prime flowcell), generating 12.66–34.30 million read pairs/ sample (median: 19.99) corresponding to coverages from 0.36 to 0.93X (median: 0.60X). Fastq files were demultiplexed using bcl2fastq (v2.20.0.422) and quality checked using fastQC and fastqScreen (Available from: http://www.bioinformatics.babraham.ac.uk/projects).

Renaud G., Nørgaard M., Lindberg J., Grönberg H., De Laere B., Jensen J.B., Borre M., Andersen C.L., Sørensen K.D., Maretty L, & Besenbacher S. (2022). Unsupervised detection of fragment length signatures of circulating tumor DNA using non-negative matrix factorization. eLife, 11, e71569.

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Comprehensive Plasma DNA Library Prep

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Libraries for next generation sequencing were prepared using the Kapa Hyper Library Preparation Kit (KAPA Biosystems) with 7.0-50.0 ng cfDNA (median 30.5 ng) or 50 ng for germline DNA as input. xGen CS-adapters -Tech Access (IDT-DNA) with Unique Molecular Identifiers (UMIs) on both strands and primers containing unique indexes on both strands were used to generate indexed libraries. Plasma libraries were pooled equimolarly and paired-end sequenced (2x151bp) on an Illumina® Novaseq instrument (S-prime flowcell), generating 12.66-34.30 million read pairs/ sample (median: 19.99) corresponding to coverages from 0.36-0.93X (median: 0.60X). Fastq files were demultiplexed using bcl2fastq (v2.20.0.422) and quality checked using fastQC and fastqScreen (Available from: http://www.bioinformatics.babraham.ac.uk/projects).

Renaud G., Nørgaard M., Lindberg J., Grönberg H., Laere B.D., Jensen J.B., Borre M., Andersen C.L., Sørensen K.D., Maretty L., & Besenbacher S. (2021). Discovering fragment length signatures of circulating tumor DNA using Non-negative Matrix Factorization.

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