Materials used to make the spheres and to measure the enzyme activity like low molecular mass chitosan, acetic acid, glutaraldehyde (GTA), p-nitrophenyl-β-d -xylopyranoside (pNPX), and birchwood xylan were from Sigma (St. Louis, MO, USA). Acrylamide, bromophenol blue, and Coomassie R-250 brilliant blue used were from Fisher Scientific (Hampton, NH, USA). Bis-Acrylamide, sodium dodecyl sulfate (SDS), glycerol, isopropyl β-d -1-thiogalactopyranoside (IPTG), molecular mass marker V849, and tris-base were from Promega (Madison, WI, USA). All other chemicals used were analytical grade reagents.
Bis acrylamide
Manufactured by Promega
Sourced in United States
Bis-acrylamide is a chemical compound used as a cross-linking agent in polyacrylamide gel electrophoresis (PAGE) applications. It functions to create a three-dimensional network structure within the polyacrylamide gel, which is essential for the separation and analysis of biomolecules such as proteins and nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
Chitosan, by Merck Group (1 mentions)
Acrylamide, by Thermo Fisher Scientific (1 mentions)
Bromophenol blue, by Thermo Fisher Scientific (1 mentions)
Sodium dodecyl sulfate (sds), by Promega (1 mentions)
P nitrophenyl β d xylopyranoside pnpx, by Merck Group (1 mentions)
Tris base, by Promega (1 mentions)
Glutaraldehyde gta, by Merck Group (1 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Promega (1 mentions)
Birchwood xylan, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Acrylamide, by Merck Group (1 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
Tetramethylenediamine, by Merck Group (1 mentions)
2 protocols using bis acrylamide
1
Enzyme Activity Assay with Chitosan Spheres
2
Acetylcholinesterase Activity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Acetylthiocholine (ASCh) iodide, butyrylthiocholine (BSCh) iodide, 5,5′-dithio-bis (c-nitrobenzoic acid) (DTNB), eserine hemisulfate salt, tetraisopropylpyrophosphoramide (iso-OMPA), 1,5-bis (4-allyldimethyl ammoniumphenyl)-pentan-3-one dibromide (BW284C51), HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), EDTA (etilen diamino-tetracetic acid), boric acid, bovine serum albumin, Tris, glycerol, bromophenol blue, maleic acid, sodium citrate dihydrate, copper pentahydrate, potassium ferricyanide, cholinesterase acetyl (True Cholinesterase EC 3.1.1.7) Type V-S from Electric Eel, acrylamide, tetramethylenediamine and ammonium persulfate were purchased from SIGMA. Sodium dodecyl sulphate (99% pure) and ethanol (99% pure) were purchased from MERCK (Germany). Bisacrylamide was acquired from PROMEGA.
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