TGA system (DTG-60H) was used to analyze the mass loss of the intermediate perovskite film during the annealing process of 150 °C. To simulate the annealing process of the intermediate perovskite film with the TGA system, the wet intermediate perovskite film was lifted off from the substrate. Firstly, intermediate perovskite films were deposited on the poly[bis(4-phenyl) (2,5,6-trimethylphenyl) amine (PTAA) substrate. Then the pristine film or humidified films were immersed into chlorobenzene (CB) for about 10 min. PTAA, acting as a sacrificial layer, was dissolved by CB resulting in a free-stand upper intermediate perovskite layer. These samples were lifted off and floated on the solvent surface. Finally, the free-standing intermediate perovskite film was loaded on the TGA balance to evaluate the mass loss during the annealing process. The sample was heated at a ramp rate of 5 °C min−1 and then kept at 150 °C for 30 min. Note that the samples were transferred with a sealable cabin and the whole measurement process was performed in N2 atmosphere. Meanwhile, the residual CB in the samples can be removed in the heating process from 70 °C to 150 °C. Finally, AFM images of the annealed perovskite films for the control and the target samples were recorded by using a Multimode 8 SPM system (Bruker).
Multimode 8 spm system
Manufactured by Bruker
The Multimode 8 SPM system is a scanning probe microscope (SPM) designed for high-resolution imaging and analysis of surfaces. The system provides a platform for various SPM techniques, including atomic force microscopy (AFM), scanning tunneling microscopy (STM), and related modes. The Multimode 8 enables users to obtain detailed topographical and material property information about samples at the nanoscale.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using multimode 8 spm system
1
Thermal Analysis of Perovskite Film Annealing
2
Nucleosome Immobilization and AFM Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The reconstituted nucleosome samples (20 ng/μL) were prepared in HE buffer and fixed with 0.1%glutaraldehyde (Fluka) on ice for 30 min. Rinse the mixture with 500 μL HE buffer for 2–3 times in a vivaspin500 column. The column was spined at 15 000 g for 2–5 min at 4 °C to get rid of the liquids and diluted to 0.5–1 ng/μL in HE buffer. 20 μL spermidine (1 mM) was dropped onto newly cleaved mica surface and incubated for 10 min. Then rinse the mica with 200 μL ddH2O for 4 times and blow to dry mica surface briefly with nitrogen gas. 10 μL sample solution was added onto the mica surface, incubated for 10–15 min. Finally, wash the mica with 200 μL ddH2O for 3 times and blow to dry gently. The prepared AFM samples were examined using ScanAsyst Mode of AFM (MultiMode 8 SPM system, BRUKER).
3
Photo-Conductive AFM Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Photo-conductive atomic force microscopy was carried out on a commercial SPM system (Bruker MultiMode 8 SPM system) with the combination of the light source of a 405 nm laser pointer. Diamond tips (Adama AD-40-AS, resonance frequency = 200 kHz) were used for photo-current detection.
4
AFM Imaging of Nanomaterials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The AFM images have been recorded on a Multimode®8 SPM System manufactured by Bruker AXS. The used cantilevers were AC200TS by Oxford Instruments with an average spring constant of 9 N m−1, an average frequency of 150 kHz, an average length of 200 µm, an average width of 40 µm and an average tip radius of 7 nm. All samples have been prepared using the protocol described below, unless specifically stated otherwise.
5
Atomic Force Microscopy of Spin-Coated Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AFM measurements were performed under ambient conditions using a Bruker Multimode 8 SPM system operating in tapping mode in air. Silica cantilevers (OMCL-AC160TS, Olympus) with a resonance frequency of ~ 300 kHz and a spring constant of ~40 Nm−1 were used. The samples were spin-coated from solution onto highly ordered pyrolytic graphite with 4000 or 10,000 rpm.
6
AFM Imaging of Reconstituted Nucleosomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reconstituted nucleosome samples were prepared in HE buffer with DNA concentrations of 20 ng/μl. The samples were fixed with 0.1% glutaraldehyde (Fluka) in HE buffer on ice for 30 min. Rinse the mixture with 500 μl HE buffer for 2–3 times in a vivaspin500 column. Spin the column at 15 000 g for 2–5 min at 4°C to get rid of the liquids. Then dilute the sample solution in HE buffer with concentrations of 0.5–1 ng/μl. Drop 20 μl spermidine (1 mM) onto newly cleaved mica surface and incubate for 10 min. Then rinse the mica with 200 μl ddH2O for 4 times and blow dry mica surface briefly with nitrogen gas. Add 10 μl sample solution onto mica surface, incubate for 10–15 min. Finally, wash the mica with 200 μl ddH2O for 3 times and blow dry gently. The prepared AFM samples were examined using ScanAsyst Mode of AFM (MultiMode 8 SPM system, BRUKER).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!