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Gt rosa 26sortm9 cag tdtomato hze j

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The Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J is a transgenic mouse line that expresses the tdTomato fluorescent protein under the control of the CAG promoter. The tdTomato protein is a bright red fluorescent protein that can be used for lineage tracing and cell labeling applications.

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Lab products found in correlation

C57bl 6j, by Jackson ImmunoResearch (3 mentions) Tdtomato, by Jackson ImmunoResearch (1 mentions) Slc32a1tm2 cre lowl j, by Jackson ImmunoResearch (1 mentions) Cba j, by Jackson ImmunoResearch (1 mentions) Tg rbp4 cre kl100gsat mmucd, by Jackson ImmunoResearch (1 mentions) Pvalbtm1 cre arbr j, by Jackson ImmunoResearch (1 mentions) 129s1 cg tg vsx2 cre 2690chow j, by Jackson ImmunoResearch (1 mentions)

7 protocols using gt rosa 26sortm9 cag tdtomato hze j

1

Mice Experiments on VGAT-Cre, Ai9 Transgenic

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Mice were 6-12 weeks old at the time of experiments. Mice were acquired from Jackson Laboratories: C57BL/6J; Slc32altm2(cre)Lowl/J (VGAT-Cre); Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (Ai9). Both female and male animals were used and housed at 21°C and 40% humidity with a reverse light cycle (12h-12h). All experiments were performed during their dark cycle. All procedures were approved and conducted in accordance with the Institutional Animal Care and Use Committee at the University of North Carolina at Chapel Hill, as well as the guidelines of the National Institutes of Health. Study results are reported in accordance with the ARRIVE guidelines.
Kline A.M., Aponte D.A, & Kato H.K. (2023). Distinct nonlinear spectrotemporal integration in primary and secondary auditory cortices. bioRxiv.
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2

Characterizing Mchr1-CreER Mouse Line

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All procedures were approved by the Institutional Animal Care and Use Committee at Indiana University Purdue University Indianapolis. Mice were housed on a standard 12-hour light dark cycle and given food and water ad libitum. Mice were weaned and housed with same-sex littermates after postnatal day 21. Ear punches were taken for genotype analysis by polymerase chain reaction.
Mchr1-CreER founders were compared to Mchr1-Cre mice (C57BL/6J-Tg(Mchr1-cre)1Emf/J, stock number 021582). Both Mchr1-CreER and Mchr1-Cre mice were crossed to Cre reporter lines, ROSALacZ (Gt(ROSA)26Sortm1Sor/J, stock number 003309 | R26R) or tdTomato (Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J, stock number 007909) (Jackson Labs; Bar Harbor, ME). Both ROSALacZ and ROSAtdTomato only express the reporter upon Cre mediated recombination. Two founders showed robust reporter expression and were further characterized. Experiments utilized both male and female mice and no differences between sexes were noted.
Engle S.E., Antonellis P.J., Whitehouse L.S., Bansal R., Emond M.R., Jontes J.D., Kesterson R.A., Mykytyn K, & Berbari N.F. (2018). A CreER mouse to study melanin concentrating hormone signaling in the developing brain. Genesis (New York, N.y. : 2000), 56(8), e23217.
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3

Mouse Strains for Neuroscience Research

Cited 2 times
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Mice were 6–12 weeks old at the time of experiments. Mice were acquired from Jackson Laboratories: C57BL/6J; CBA/J; Slc32a1tm2(cre)Lowl/J (VGAT-Cre); Pvalbtm1(cre)Arbr/J (PV-Cre); Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (Ai9). For Fig. 6, data from our previous studies were reanalyzed (Aponte et al. 2021 (link); Kline et al. 2021 (link); Onodera and Kato 2022 ). For this dataset, additional mice were acquired from Jackson Laboratories (Ssttm2.1(cre)Zjh/J (Sst-Cre)) and MMRRC (Tg(Rbp4-Cre)KL100Gsat/Mmucd (Rbp4-Cre); Tg(Tlx3-Cre)PL56Gsat/Mmucd (Tlx3-Cre)). Both female and male animals were used and housed at 21 °C and 40% humidity with a reverse light cycle (12–12 h). All experiments were performed during their dark cycle. All procedures were approved and conducted in accordance with the Institutional Animal Care and Use Committee at the University of North Carolina at Chapel Hill as well as the guidelines of the National Institutes of Health.
Narayanan D.P., Tsukano H., Kline A.M., Onodera K, & Kato H.K. (2022). Biological constraints on stereotaxic targeting of functionally-defined cortical areas. Cerebral Cortex (New York, NY), 33(6), 3293-3310.
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4

VGAT-Cre Mouse Model Experiments

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Mice were 6–12 weeks old at the time of experiments. Mice were acquired from Jackson Laboratories: C57BL/6J; Slc32a1tm2(cre)Lowl/J (VGAT-Cre); Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (Ai9). Both female and male animals were used and housed at 21 °C and 40% humidity with a reverse light cycle (12–12 h). All experiments were performed during their dark cycle. All procedures were approved and conducted in accordance with the Institutional Animal Care and Use Committee at the University of North Carolina at Chapel Hill, as well as the guidelines of the National Institutes of Health. Study results are reported in accordance with the ARRIVE guidelines.
Kline A.M., Aponte D.A, & Kato H.K. (2023). Distinct nonlinear spectrotemporal integration in primary and secondary auditory cortices. Scientific Reports, 13, 7658.
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5

Genetic Manipulation of Inhibitory Neurons

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Mice were bred at the University of Colorado Anschutz and were from a B6;129 or B6.Cg mixed genetic background. Pvalbtm1(cre)Arbr/J homozygote mice (“PV-IRES-Cre,” Jax 008069) breeders were kindly provided by Dr. Diego Restrepo and Nrxn3tm3Sud/J (“Nrxn3 cKO,” Jax 014157) breeders were a generous gift from Dr. Thomas Südhof. Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (“Ai9”: Jax 007909) mice were obtained from The Jackson Laboratory. Mice were housed in a dedicated animal care facility maintained at 35% humidity, 21–23°C, on a 14/10 light/dark cycle. Mice were housed in groups of 2–5 in ventilated cages with same-sex littermates with food and water ad libitum. Mice were genotyped in-house, and sex of the animal was determined by external genitalia. Animals were randomly selected for experiments. Animals were stereotactically injected at P21–22, and all other experiments were performed at P35–42 in visibly healthy animals. All procedures were conducted in accordance with guidelines approved by Administrative Panel on Laboratory Animal Care at University of Colorado, Anschutz School of Medicine, accredited by Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC) (00235).
Boxer E.E., Seng C., Lukacsovich D., Kim J., Schwartz S., Kennedy M.J., Földy C, & Aoto J. (2021). Neurexin-3 defines synapse- and sex-dependent diversity of GABAergic inhibition in ventral subiculum. Cell reports, 37(10), 110098.
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6

Genetic Manipulation of Inhibitory Neurons

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Mice were bred at the University of Colorado Anschutz and were from a B6;129 or B6.Cg mixed genetic background. Pvalbtm1(cre)Arbr/J homozygote mice (“PV-IRES-Cre,” Jax 008069) breeders were kindly provided by Dr. Diego Restrepo and Nrxn3tm3Sud/J (“Nrxn3 cKO,” Jax 014157) breeders were a generous gift from Dr. Thomas Südhof. Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (“Ai9”: Jax 007909) mice were obtained from The Jackson Laboratory. Mice were housed in a dedicated animal care facility maintained at 35% humidity, 21–23°C, on a 14/10 light/dark cycle. Mice were housed in groups of 2–5 in ventilated cages with same-sex littermates with food and water ad libitum. Mice were genotyped in-house, and sex of the animal was determined by external genitalia. Animals were randomly selected for experiments. Animals were stereotactically injected at P21–22, and all other experiments were performed at P35–42 in visibly healthy animals. All procedures were conducted in accordance with guidelines approved by Administrative Panel on Laboratory Animal Care at University of Colorado, Anschutz School of Medicine, accredited by Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC) (00235).
Boxer E.E., Seng C., Lukacsovich D., Kim J., Schwartz S., Kennedy M.J., Földy C, & Aoto J. (2021). Neurexin-3 defines synapse- and sex-dependent diversity of GABAergic inhibition in ventral subiculum. Cell reports, 37(10), 110098.
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7

Genetic Mouse Models for CBC Identification

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The following mouse strains were obtained from the Jackson Laboratory: 129S1.Cg-Tg(Vsx2-cre)2690Chow/J (RRID:IMSR_JAX:026200) and Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (RRID:IMSR_JAX:007909; Ai9 hereafter). We also used the Tg(Gnat3-GFP)1Rfm/ChowJ (RRID:IMSR_JAX:026704; Gustducin-gfp hereafter) reporter mouse (Huang et al., 2003 (link)), originally obtained from the laboratory of Richard Masland, and currently available from the Jackson Laboratory. Sox5tm2Vlf mice (RRID:MGI:3800352), carrying a floxed allele of Sox5, were obtained from the laboratory of Véronique Lefebvre at The Cleveland Clinic (Dy et al., 2008 (link)). These lines were bred and then crossed to yield Sox5-conditional knockout (CKO) mice additionally reporting Cre activation as well as selectively identifying Type 7 CBCs.
Kulesh B., Reese B.E, & Keeley P.W. (2022). Contraction of axonal and dendritic fields in Sox5-deficient cone bipolar cells is accompanied by axonal sprouting and dendritic hyper-innervation of pedicles. Frontiers in Neuroanatomy, 16, 944706.
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