Nexera x2 lcms 8050

We analyzed changes in the concentration of amino acids and glucose in plasma-treated cell culture medium using an LC-MS/MS system (Shimadzu Corp., Nexera X2/LCMS-8050) as described previously with slight modifications^{45 (link)}. The LC/MS/MS Method Package for Cell Culture Profiling (Shimadzu Corp.) provides optimized analytical conditions for up to 95 compounds, either as basal cell culture media components or as secreted metabolites, using LC-MS/MS data. In addition, changes in the mass spectrum of an amino acid in plasma-treated amino acid solution were analyzed with a liquid chromatography mass spectrometer (Shimazu Corp., LCMS-2020). The operating conditions of the electrospray ionization mass spectrometer (ESI-MS) were as follows: (1) electrospray potential = 4.50 kV, (2) capillary voltage = 0 V, (3) capillary temperature = 250 °C, (4) detector voltage = −1.30 kV, (5) skimmer voltage = 0 V, and (6) m/z scan range = 10–300, where m and z represent mass and charge number, respectively. The mass resolution of the LCMS-2020 as defined by m/Δm_50% is 2 m, where Δm_50% is the peak width at 50% of the maximum peak intensity⁴⁶ .

The validation of beta-lactam antibiotics for routine 24/7 clinical diagnosis was performed using the CLAM-2000 (Shimadzu Corporation, Kyoto, Japan) connected to a UHPLC-MS/MS system (NexeraX2-LC-MS-8050, Shimadzu, Kyoto, Japan). CLAM-2000 (in combination with LabSolutions software, version 5.1.09) was used for instrument control, data acquisition, and processing.