Donkey anti rabbit igg dylight 549

Manufactured by Jackson ImmunoResearch

Sourced in United States

Donkey-anti–rabbit IgG DyLight 549 is a secondary antibody reagent. It is produced by immunizing donkeys with rabbit immunoglobulin G (IgG) and conjugating the resulting antibodies to the DyLight 549 fluorescent dye.

Automatically generated - may contain errors

Lab products found in correlation

Streptavidin alexa 647, by Thermo Fisher Scientific (1 mentions) Streptavidin alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Cd4 apc rm4 5, by Thermo Fisher Scientific (1 mentions) Cd11c biotin, by BioLegend (1 mentions) Sirpα, by BioLegend (1 mentions) Donkey anti rat igg dylight488, by Jackson ImmunoResearch (1 mentions) Anti keratin 5, by Fortrea (1 mentions) Anti p21cip1 sc 397, by Santa Cruz Biotechnology (1 mentions) Anti p53 sc 126, by Santa Cruz Biotechnology (1 mentions) Alexa flour 488 conjugated goat anti mouse, by Thermo Fisher Scientific (1 mentions) Anti p65 sc 8008, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

DyLight 549 (41 citations) DyLight 549 anti-rabbit IgG (2 citations) Rabbit anti-IgG (2 citations) Rabbit IgGs (2 citations)

2 protocols using donkey anti rabbit igg dylight 549

Multiparametric Analysis of Thymocyte Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

For flow cytometry and FACS purification of thymocyte subsets and thymic stroma, the following fluorochrome or biotin-conjugated antibodies (from eBioscience or BioLegend unless indicated) were used: anti-CD3 (145-2C11), -CD4 (RM4-5), -CD8 (53–6.7), -CD69 (H1.2F3), -CD25 (PC61.5), -CD45.1 (A20), -CD45.2 (104), -Vβ5 (MR9-4), -Foxp3 (FJK-16s), -EpCAM (G8.8), -TER-119 (TER-119), -CD11c (N418), -CD31 (390), -Sirpα (P84), -B220 (RA3-6B2), -I-A/I-E (M5/114.15.2), -CD80 (16-10A1), -CD45 (30-F11; BD), -Gr1 (RB6-8C5), -CD11b (M1/70), -NK1.1 (PK136), -cKit (2B8), and Ly-51 (6C3).
For immunofluorescent analyses, the following antibodies were used: anti-keratin 5 (polyclonal; Covance), -pan-cytokeratin (C-11; Sigma-Aldrich), -CD8-Alexa Fluor 488 (53–6.7; eBioscience), -CD4-APC (RM4-5; eBioscience), -CD4 (GK1.5; Bio X cell), -CD69-Biotin (H1.2F3; BioLegend), -CD11c-Biotin (N418; BioLegend), -Sirpα (P84; BioLegend), donkey-anti–rabbit IgG DyLight 549 (poly-clonal; Jackson ImmunoResearch Laboratories), and Donkey-anti–rat IgG DyLight 488 (polyclonal; Jackson ImmunoResearch Laboratories), Streptavidin Alexa Fluor 488 (Life Technologies), and Streptavidin Alexa 647 (Life Technologies).

Hu Z., Lancaster J.N., Sasiponganan C, & Ehrlich L.I. (2015). CCR4 promotes medullary entry and thymocyte–dendritic cell interactions required for central tolerance. The Journal of Experimental Medicine, 212(11), 1947-1965.

+ Open protocol

+ Expand

Senescence Induction in Lymphoma Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

HL-derived L428 and KMH2 cells were grown in suspension in RPMI 1640 supplemented with 100 U/ml penicillin, 0.1 mg/ml streptomycin, 2 mM L-glutamine and 10% fetal calf serum (FCS), in a 5% CO2 humidified atmosphere at 37 °C. For the induction of senescence cells were treated with 50 μM H₂O₂ for 2 h in RPMI without FCS, washed with complete medium and grown for 4–7 days.
Antibodies used included: anti-p21 ^Cip1 (sc-397), anti-p16 ^INK4a (sc-1207), anti-p53 (sc-126) and anti-p65 (sc-8008) and phospho-NF-κB p65 (Ser 536) (sc-33020- R) which were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), anti-γH2AX phosphor -S139 (ab18311, Abcam, Cambridge, MA, USA), anti-trimethyl Histone H3 (Lys4/Lys9) (Millipore 07-992). Anti CD15 LeuM1 and anti-CD30 BerH2 (Dako, Copenhagen, Denmark), Ki-67, (ab15580, Abcam). For florescence detection the following secondary antibodies were used: donkey anti-rabbit IgG (DyLight 549, 711-505-152, Jackson ImmunoResearch Laboratories, West Grove, PA, USA), Alexa flour-488 conjugated goat anti-mouse (Molecular Probes Inc., Eugene, OR, USA).

Gopas J., Stern E., Zurgil U., Ozer J., Ben-Ari A., Shubinsky G., Braiman A., Sinay R., Ezratty J., Dronov V., Balachandran S., Benharroch D, & Livneh E. (2016). Reed-Sternberg cells in Hodgkin's lymphoma present features of cellular senescence. Cell Death & Disease, 7(11), e2457-.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!