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Ab105134

Manufactured by Abcam
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Ab105134 is a lab equipment product. It is a buffer solution used for preparing samples for analysis.

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Lab products found in correlation

Ab105135, by Abcam (19 mentions) Ab83369, by Abcam (2 mentions) Ab108791, by Abcam (2 mentions) Ab65340, by Abcam (2 mentions) Ab83362, by Abcam (2 mentions) Ab211096, by Abcam (2 mentions) Ab252904, by Abcam (2 mentions) Eiagshc, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Molecular Devices (1 mentions) Ab179800, by Abcam (1 mentions) Bovine albumin, by Merck Group (1 mentions) Ab15580, by Abcam (1 mentions) Ab32503, by Abcam (1 mentions) Folin s reagent, by Merck Group (1 mentions) Anti proliferating cell nuclear antigen, by Cell Signaling Technology (1 mentions) Anti caspase 9, by Novus Biologicals (1 mentions) Anti caspase 3, by Cell Signaling Technology (1 mentions) Pentobarbital sodium, by Merck Group (1 mentions) Ab16502, by Abcam (1 mentions) Ab32572, by Abcam (1 mentions)

31 protocols using ab105134

1

Liver Enzyme and Glutathione Assays

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The levels of ALT or AST in mouse serum and hepatocyte supernatant were measured using ALT (ab105134, Abcam) or AST (ab105135, Abcam) Activity Assay Kits. Liver GSH measurements were performed using the Glutathione Colorimetric Detection Kit (EIAGSHC, Thermo Fisher). All colorimetric assays were performed according to the manufacturer’s protocol.
Dong J., Viswanathan S., Adami E., Schafer S., Kuthubudeen F.F., Widjaja A.A, & Cook S.A. (2021). The pro-regenerative effects of hyperIL6 in drug-induced liver injury are unexpectedly due to competitive inhibition of IL11 signaling. eLife, 10, e68843.
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2

Serum Enzyme Assay Protocol

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Blood samples were centrifuged at 3000 rpm for 10 min and the sera were collected. Alanine aminotransferase and aspartate aminotransferase levels were then assayed with commercial kits (ab105134 and ab105135, Abcam, Cambridge, UK) according to the manufacturer's protocols.
Liu G., Wang Q., Deng L., Huang X., Yang G., Cheng Q., Guo T., Guo L., Niu C., Yang X., Dong J, & Zhang J. (2022). Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells. Theranostics, 12(5), 2248-2265.
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3

Colorimetric Assays for Liver and Kidney Markers

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Plasma alanine aminotransferase (ALT; ab105134) and aspartate aminotransferase (AST; ab105135) were measured with a colorimetric assay, using reagents from Abcam Inc. (Cambridge, UK). Lactate dehydrogenase (LDH; K726) activity and blood urea nitrogen (BUN; ABIN577679) were analyzed using detection kit according to the manufacturer's instructions, which kits were obtained from BioVision, Inc. (Milpitas, CA, USA) and antibodies‐online GmbH (Aachen, Germany), respectively.
Choi E.O., Lee H., HwangBo H., Kwon D.H., Kim M.Y., Ji S.Y., Hong S.H., Kim G., Park C., Hwang H., Moon S., Yun S., Kim W, & Choi Y.H. (2019). Citrus unshiu peel suppress the metastatic potential of murine melanoma B16F10 cells in vitro and in vivo. Phytotherapy Research, 33(12), 3228-3241.
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4

Serum ALT and AST Measurement

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Serum ALT and AST levels were measured in fresh samples using the alanine transaminase and aspartate aminotransferase activity assay kits (ab105134 and ab105130, respectively, Abcam, Cambridge, UK), respectively, according to the manufacturer’s instructions.
Wollman A., Daniel T, & Rosenzweig T. (2019). Sarcopoterium spinosum Inhibited the Development of Non-Alcoholic Steatosis and Steatohepatitis in Mice. Nutrients, 11(12), 3044.
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5

Quantifying Liver Enzyme Activity

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ALT and AST activity was determined using kits (ab105134, ab105135) from Abcam.
Xiao K., Zhang D.C., Hu Y., Song L.C., Xu J.Q., He W.X., Pan P., Wang Y.W, & Xie L.X. (2022). Potential roles of vitamin D binding protein in attenuating liver injury in sepsis. Military Medical Research, 9, 4.
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6

Quantitative ELISA for Hamster Liver Enzymes

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Hamster ALP (MBS011057) and ALT (MBS011423) quantitative sandwich ELISA kits were procured from MyBioSource, Inc. (San Diego, CA) and used to measure ALP and ALT levels, respectively, in serum as per the manufacturer’s instructions. Colorimetric assays (p-nitrophenyl and pyruvate based) were used to measure ALP (ab83369, Abcam, Cambridge, MA) and ALT (ab105134) activities, respectively, in serum and calculated as per the manufacturer’s instructions. All plates were read in a microplate reader form Molecular Devices (San Jose, CA).
Plante K.S., Dwivedi V., Plante J.A., Fernandez D., Mirchandani D., Bopp N., Aguilar P.V., Park J.G., Tamayo P.P., Delgado J., Shivanna V., Torrelles J.B., Martinez-Sobrido L., Matos R., Weaver S.C., Sastry K.J, & Newman R.A. (2021). Antiviral activity of oleandrin and a defined extract of Nerium oleander against SARS-CoV-2. Biomedicine & Pharmacotherapy, 138, 111457.
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7

Evaluating Liver Function in Mice

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Blood samples were drawn from the hearts of anesthetized mice at 14 d after transplantation. Serum was separated by centrifugation at 3,000 rpm for 15 min and stored at −80°C until analysis. To test liver function after TAA treatment, ALT and AST activity was measured using an automatic chemistry analyzer (Hitachi 7070) according to the manufacturer’s instructions. Alternatively, AST (ab105135; Abcam) and ALT (ab105134; Abcam) assays were performed using the colorimetric method in TG mice. The substrates, glutamate and pyruvate, were used for generating standard curves to measure enzyme amounts. AST and ALT activities were determined following the manufacturer’s procedure and were expressed as milliunits per milliliter.
Lee E.J., Hwang I., Lee J.Y., Park J.N., Kim K.C., Kim I., Moon D., Park H., Lee S.Y., Kim H.S., Jun D.W., Park S.H, & Kim H.S. (2020). Hepatic stellate cell–specific knockout of transcriptional intermediary factor 1γ aggravates liver fibrosis. The Journal of Experimental Medicine, 217(6), e20190402.
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8

Hepatoprotective Effects Evaluation Protocol

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CR, DEN, Folin’s reagent, sodium pentobarbital, and bovine albumin were sourced from Sigma Chemical Co. (St. Louis, MO, USA). The alanine transaminase (ALT) (ab105134) and aspartate aminotransferase (AST) (ab105135) kits were purchased from Abcam (Cambridge, CB2 0AX, UK). Rabbit monoclonal antibodies against Bax [E63] (ab32503), β-catenin [E247] (ab32572,) COX2 [EPR12012] (ab179800), Ki67 antibody (ab15580), and NF-κB-p65 (ab16502) were purchased from Abcam (Cambridge, CB2 0AX, UK). Anti-proliferating cell nuclear antigen (PCNA), and anti-caspase-3 were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Anti-caspase-9 was sourced from Novus Biologicals (Littleton, CO, USA), and anti-poly ADP-ribose polymerase (PARP), anti-Bcl-2, and anti-Bax for Western Blotting (WB) investigation were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). SB was purchased from Carbosynth Ltd. (Compton, UK).
Awad B., Hamza A.A., Al-Maktoum A., Al-Salam S, & Amin A. (2023). Combining Crocin and Sorafenib Improves Their Tumor-Inhibiting Effects in a Rat Model of Diethylnitrosamine-Induced Cirrhotic-Hepatocellular Carcinoma. Cancers, 15(16), 4063.
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9

Metabolic Profiling in Fasted Mice

Cited 1 time
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Mice were fasted overnight and retro-orbital blood was drawn beginning at 11:00 a.m. the following morning to assess steady-state levels of plasma insulin (Mouse Ultrasensitive ELISA kit, Alpco, Salem, NH), C-peptide (ELISA, Alpco), non-esterified fatty acids (NEFA C kit, Wako Diagnostics, Richmond, VA), triacylglycerol (Pointe Scientific Triglyceride), endothelin-1 (ELISA kit, ab133030, Abcam, Cambridge, MA), TNFα (SimpleStep ELISA kit, ab208348, Abcam) and IL-6 (ELISA Kit, ab222503, Abcam). Hepatic triacylglycerol was measured as previously described [26 (link)], hepatic nitric oxide (NO) levels using Nitrate/Nitrite Fluorometric Assay (780051, Cayman Chemical, Ann Arbor, MI), and hepatic levels of reduced glutathione (GSH) were measured using the Bioxytech GSH-400 kit (OXISResearch, Portland, OR). Plasma Alanine Transaminase (ALT) (ab105134, Abcam) and Aspartate Aminotransferase (AST) (ab105135) colorimetric assays kits were used to assess liver function.
Helal R.A., Russo L., Ghadieh H.E., Muturi H.T., Asalla S., Lee A.D., Gatto-Weis C, & Najjar S.M. (2021). Regulation of hepatic fibrosis by Carcinoembryonic Antigen-related Cell Adhesion Molecule 1. Metabolism: clinical and experimental, 121, 154801.
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10

Serum Biomarker Measurement Protocol

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Serum glucose was measured using a FreeStyle Optium Neo Blood Glucose Meter (Abbott Laboratories). Commercially available kits were used to determine serum insulin (ALPCO, Cat No. 80-INSMSU-E01), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (Abcam, ab105134 and ab105135). The serum was diluted 1:25 for insulin analysis and 1:10 for ALT and AST. Experiments were conducted according to the manufacturer’s instructions.
Meggyesy P.M., Masaldan S., Clatworthy S.A., Volitakis I., Eyckens D.J., Aston-Mourney K, & Cater M.A. (2020). Copper Ionophores as Novel Antiobesity Therapeutics. Molecules, 25(21), 4957.
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