Cd10 bv421

Manufactured by BD

Sourced in United States

The CD10-BV421 is a fluorochrome-conjugated antibody that targets the CD10 cell surface antigen. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Cd19 bv711, by BD (5 mentions) Cd21 fitc, by BD (5 mentions) Cd20 alexafluor700, by BD (5 mentions) Cd3 pe, by BD (5 mentions) Cd27 percp cy5, by BD (5 mentions) Lsrfortessa x 20 flow cytometer, by BD (4 mentions) Facsdiva software, by BD (2 mentions) Facsdiva software v7, by BD (1 mentions) Facsdiva software v6, by BD (1 mentions)

5 protocols using cd10 bv421

Phenotyping Human B Cell Subsets

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B cells are CD3^-CD19⁺ cells with different phenotypes that may be determined by the analysis of the expression of surface markers CD10, CD27 and CD21 (16 (link)): immature or transitional cells (CD10⁺ CD27^-); naïve B cells (CD10^-CD27^-CD21^high); tissue-like memory cells (CD10^-CD27^-CD21^low); resting memory cells (CD10^-CD27⁺CD21^high); activated memory cells (CD10^-CD27⁺CD21^low); and plasmablasts (CD27⁺⁺CD20^-CD21^low) (16 (link)). Antibodies CD3-PE, CD10-BV421, CD19-BV711, CD20-AlexaFluor700, CD21-FITC, CD27-PercP-Cy5.5 were purchased from BD Biosciences (San Jose, CA). Data acquisition and analysis was performed as described above. The gating strategy for acquiring and analyzing the B cell subsets is described in Supplemental Figure 1.

Vigón L., García-Pérez J., Rodríguez-Mora S., Torres M., Mateos E., Castillo de la Osa M., Cervero M., Malo De Molina R., Navarro C., Murciano-Antón M.A., García-Gutiérrez V., Planelles V., Alcamí J., Pérez-Olmeda M., Coiras M, & López-Huertas M.R. (2021). Impaired Antibody-Dependent Cellular Cytotoxicity in a Spanish Cohort of Patients With COVID-19 Admitted to the ICU. Frontiers in Immunology, 12, 742631.

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Flow Cytometry Analysis of B Cell Subsets

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Subpopulations of B cells (CD3⁻CD19⁺) were analyzed by flow cytometry after staining of surface markers CD10, CD27, CD20, and CD21: immature or transitional cells (CD10⁺ CD27⁻), naïve B cells (CD10⁻CD27⁻CD21^high), tissue-like memory cells (CD10⁻CD27⁻CD21^low), resting memory cells (CD10⁻CD27⁺CD21^high), activated memory cells (CD10⁻CD27⁺CD21^low), and plasmablasts (CD27⁺⁺CD20⁻CD21^low) [35 (link)]. Antibodies CD3-PE, CD10-BV421, CD19-BV711, CD20-AlexaFluor700, CD21-FITC, and CD27-PercP-Cy5.5 were purchased from BD Biosciences (San Jose, CA, USA). Data acquisition was performed in a BD LSRFortessa X-20 flow cytometer with FACS Diva software (BD Biosciences, San Jose, CA, USA). FlowJo software (Tree Star Inc., Ashland, OR, USA) was used for data analysis.

Torres M., Corona M., Rodríguez-Mora S., Casado-Fernández G., Zurdo-Castronuño A., Mateos E., Ramos-Martín F., Sánchez-Menéndez C., Murciano-Antón M.A., García-Pérez J., Alcamí J., Pérez-Olmeda M., Coiras M., López-Jiménez J, & García-Gutiérrez V. (2022). Strong Humoral but Not Cellular Immune Responses against SARS-CoV-2 in Individuals with Oncohematological Disease Who Were Treated with Rituximab before Receiving a Vaccine Booster. Cancers, 14(22), 5537.

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Characterization of B Cell Subpopulations

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Subpopulations of B cells (CD3-CD19+) were analyzed by flow cytometry after the staining of surface markers CD10, CD27, CD20, and CD21 [12 (link)]. These cells include immature or transitional cells (CD10+ CD27-), naïve B cells (CD10-CD27-CD21high), tissue-like memory cells (CD10-CD27-CD21low), resting memory cells (CD10-CD27+CD21high), activated memory cells (CD10-CD27+CD21low), and plasmablasts (CD27++CD20-CD21low). Antibodies CD3-PE, CD10-BV421, CD19-BV711, CD20-AlexaFluor700, CD21-FITC, and CD27-PercP-Cy5.5 were purchased from BD Biosciences (San Jose, CA, USA). Data acquisition was performed in BD LSRFortessa X-20 flow cytometer with FACS Diva software (BD Biosciences, San Jose, CA, USA). FlowJo software (Tree Star Inc., Ashland, OR, USA) was used for data analysis.

Vigón L., Sánchez-Tornero A., Rodríguez-Mora S., García-Pérez J., Corona de Lapuerta M., Pérez-Lamas L., Casado-Fernández G., Moreno G., Torres M., Mateos E., Murciano-Antón M.A., Alcamí J., Pérez-Olmeda M., López-Jiménez J., García-Gutiérrez V, & Coiras M. (2022). Strong Cellular Immune Response, but Not Humoral, against SARS-CoV-2 in Oncohematological Patients with Autologous Stem Cell Transplantation after Natural Infection. Journal of Clinical Medicine, 11(8), 2137.

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Comprehensive B-cell Subset Profiling

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B-cell subpopulations (CD3⁻CD19⁺) were differentiated via flow cytometry after staining the surface markers of CD10, CD27, CD20, and CD21 as follows: immature or transitional cells (CD10⁺CD27⁻); naïve B cells (CD10⁻CD27⁻CD21^high); tissue-like memory cells (CD10⁻CD27⁻CD21^low); resting memory cells (CD10⁻CD27⁺CD21^high); activated memory cells (CD10⁻CD27⁺CD21^low); and plasmablasts (CD27⁺⁺CD20⁻CD21^low). Antibodies CD3-PE, CD10-BV421, CD19-BV711, CD20-AlexaFluor700, CD21-FITC, and CD27-PercPCy5.5 were purchased from BD Biosciences (San Jose, CA, USA). Data acquisition was performed in a BD LSRFortessa X-20 flow cytometer with FACS Diva software v7.0 (BD Biosciences, San Jose, CA, USA). FlowJo software v10 (Tree Star Inc., Ashland, OR, USA) was used for data analysis.

Rodríguez-Mora S., Corona M., Solera Sainero M., Mateos E., Torres M., Sánchez-Menéndez C., Casado-Fernández G., García-Pérez J., Pérez-Olmeda M., Murciano-Antón M.A., López-Jiménez J., Coiras M, & García-Gutiérrez V. (2023). Regular Humoral and Cellular Immune Responses in Individuals with Chronic Myeloid Leukemia Who Received a Full Vaccination Schedule against COVID-19. Cancers, 15(20), 5066.

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Characterization of B Cell Subsets by Flow Cytometry

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Subpopulations of B cells (CD3⁻CD19⁺) were analyzed by flow cytometry after staining of surface markers CD10, CD27, CD20, and CD21 as follows: immature or transitional cells (CD10⁺CD27⁻); naïve B cells (CD10⁻CD27⁻CD21^high); tissue-like memory cells (CD10⁻CD27⁻CD21^low); resting memory cells (CD10⁻CD27⁺CD21^high); activated memory cells (CD10⁻CD27⁺CD21^low); and plasmablasts (CD27⁺⁺CD20⁻CD21^low) [31 (link)]. Antibodies CD3-PE, CD10-BV421, CD19-BV711, CD20-AlexaFluor700, CD21-FITC, and CD27-PercP-Cy5.5 were purchased from BD Biosciences (San Jose, CA). Data acquisition was performed in a BD LSRFortessa X-20 flow cytometer and FACS Diva software v6.0 (BD Biosciences, San Jose, CA, USA). FlowJo software v10.8 (Tree Star Inc., Ashland, OR, USA) was used for data analysis.

Rodríguez-Mora S., Corona M., Torres M., Casado-Fernández G., García-Pérez J., Ramos-Martín F., Vigón L., Manzanares M., Mateos E., Martín-Moro F., Zurdo-Castronuño A., Murciano-Antón M.A., Alcamí J., Pérez-Olmeda M., López-Jiménez J., García-Gutiérrez V, & Coiras M. (2022). Early Cellular and Humoral Responses Developed in Oncohematological Patients after Vaccination with One Dose against COVID-19. Journal of Clinical Medicine, 11(10), 2803.

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