GC–MS analysis was performed on a GCMS-QP2010 Plus mass spectrometer linked to a GC-2010 gas chromatography system (Shimadzu, Kyoto, Japan) equipped with an Rxi-5ms column (30 m × 0.25 mm ID, phase thickness 0.25 mm; Restek, Bellefonte, PA, USA). Helium was used as the carrier gas, at a flow rate of 1.5 mL min−1 in splitless mode. The program used for the analysis was 80 °C for 1 min, followed by an increase to 280 °C at 8 °C min−1. The other conditions were as follows: ion source temperature 200 °C, injection port temperature 230 °C, detector temperature 250 °C and interface temperature 280 °C. Methyl 4-PB (me4-PB) and meMMPB were detected by using selected-ion monitoring (SIM) mode. Ions at 91 and 104 m/z were selected for me4-PB, and those at 75, 78, 91, 131, and 134 m/z were selected for meMMPB [36] (link). GC–MS solution software was used for the quantitative calculation. Duplicate samples with duplicate analysis (yielded 4 measurements, n = 4) were used in this determination.
Gc 2010 gas
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu GC-2010 is a gas chromatograph that is designed for the analysis of volatile organic compounds. It features a high-performance oven, a sensitive flame ionization detector (FID), and a reliable auto-injector system. The GC-2010 is capable of providing precise and reproducible results for a wide range of applications in various industries.
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5 protocols using gc 2010 gas
1
GC-MS Analysis of Methyl Compounds
2
Quantification of Volatile Fatty Acids via GC
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Volatile fatty acids (VFAs) concentrations were quantified using a Shimadzu GC 2010 gas (Kyoto, Japan) chromatograph equipped with flame ionisation detection. A nitroterephthalic-acid-modified polyethylene glycol (PEG) column (DB-FFAP, 30.0 m × 0.53 mm × 0.5 μm, Agilent Technologies, Santa Clara, California, US) was used. Helium gas was utilised as the carrier gas. The column was first heated to 40 °C, and the temperature was held for 2 min after sample injection followed by a temperature ramp-up to 180 °C at a rate of 10 °C/min, and the temperature was maintained for 26 min. Peak identification was achieved by calibrating reference standard solutions to sample peak retention times.
3
Fatty Acid Composition Analysis Protocol
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The fatty acid composition analysis was based on LST EN ISO 12966-2:2011 [40 ] and LST EN ISO 15304:2003 [41 ]. To prepare the fatty acid methyl esters (FAME), fatty acids were extracted using a 20 mL n-heksane and saponified with a 2M KOH methanol solution. The fatty acid composition was analyzed using a Shimadzu GC-2010 gas chromatograph. It was equipped with a BPX-70, 120 m capillary column. Nitrogen was used as a carrier gas. The split ratio was 1:30. The injection temperature was 250 °C. The oven temperature was programmed according to the following sequence: 2 min at 60 °C and increasing up to 230 °C at 20 °C/min and 45 min at 230 °C. Detector temperature was 270 °C. The ionization voltage was 70 eV; the scanning range was 50–550 m/z. “Supelco 37 Component FAME Mix” (Sigma-Aldrich) was used as a standard for FFA identification, and C13:1 tetradecadiene (C14:2) and hexadecadiene (C16:2) fatty acids were identified by interpolation.
4
Fatty Acid Composition Analysis
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Tested samples (15.0g each) were ground and their oil was separated utilizing Soxhlet contraption (Gerhardt) as indicated by AOCS Official strategies Am 2-93 (Bartzatt, & Martinez, 2015) (link). The removed oil was methylated and changed over to methyl esters of the fatty acids as per the past technique, and afterwards was investigated utilizing a Shi-madzu GC-2010 gas chromatograph with a DB-23 column (60m x 0.25mm i.d. also, 0.25 µm film thickness). Injector, column and indicator temperatures were 230, 190 and 240 °C, individually. The split proportion was 80:1. Transporter gas was helium at 1.0 ml/min proportion.
5
Fatty Acid Methyl Ester Analysis
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FFAs were converted to their methyl esters according to method suggested by Hammond and Wang 12) with slight modifications. The esters were injected into GC-2010 gas chromatography (Shimadzu, Japan) through a 30 m DB-23 capillary column (Agilent, CA, USA) . Helium was the carrier and a method with 230℃ injection block temperature, 190℃ column temperature, 240℃ flame ionization detector (FID) temperature, 1 µL injection volume and 0.3 mL min -1 column flow was used.
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