Mouse stereotaxic instrument

Manufactured by Stoelting

Sourced in United States

The mouse stereotaxic instrument is a precision device used to immobilize and position a mouse's head during neuroscience research procedures. It allows for the accurate targeting of specific brain regions for various experimental applications.

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Lab products found in correlation

Prism 5, by GraphPad (1 mentions) Living image software, by PerkinElmer (1 mentions) Quintessential stereotaxic injector, by Stoelting (1 mentions)

Close spelling variants of this product

Digital Mouse Stereotaxic Instrument Digital Just for Mouse Stereotaxic Instrument Stereotaxic instrument

4 protocols using mouse stereotaxic instrument

Murine Glioblastoma Xenograft Model

Cited 1 time

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10⁵ cells of the exponentially expanding murine Ink4a/Arf(−/−) EGFRvIII or G12 cells were injected into 4-5weeks old athymic nude mice using a mouse stereotaxic instrument (Stoelting Co, Wood Dale, IL) according to the protocol previously described [45 (link)]. Survival was recorded until the onset of neurologic sequelae or cachexia. Mice were euthanized in accordance with our institutional guidelines for animal welfare and experimental conduct. The survival curve was calculated by GraphPad Prism 5 (GraphPad, La Jolla, CA) using the method of Kaplan-Meier. p values were analyzed using the logrank (Mantel-Cox) test.
TMZ was given at 15 mg/kg by oral gavage once per day for three days at indicated time point after implantation. BI2536 was given consecutively at 25 mg/kg by i.v. injection twice per week for four weeks beginning at indicated time point. Gefitinib was administered via oral gavage at 200 mg/kg weight once per day, 5 days per week for four weeks. For all experiments, 5-6 mice were randomized to each treatment group.

Shen Y., Li J., Nitta M., Futalan D., Steed T., Treiber J.M., Taich Z., Stevens D., Wykosky J., Chen H.Z., Carter B.S., Becher O.J., Kennedy R., Esashi F., Sarkaria J.N., Furnari F.B., Cavenee W.K., Desai A, & Chen C.C. (2015). Orthogonal targeting of EGFRvIII expressing glioblastomas through simultaneous EGFR and PLK1 inhibition. Oncotarget, 6(14), 11751-11767.

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Preclinical Evaluation of Glioma Therapy

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All animal protocols in our experiments were conducted in accordance with the Southwest Medical University animal welfare guidelines and approved by the Institutional Animal Care and Use Committee of the Affiliated Hospital of Southwest Medical University. For tumorigenesis analysis, male nude mice (6–8 weeks old) were purchased from the Beijing HFK company (Beijing, China). The animals were randomly assigned to groups (n = 10/group) and subcutaneously inoculated in the right flank with 5 × 10⁴ cells. Tumor numbers were counted 30 days after injection. For the orthotopic tumor model, male nude mice (6–8 weeks old) were intracranially injected with 1 × 10⁶ luciferase-marked glioma cells using a mouse stereotaxic instrument (Stoelting, CA, USA). Mice were randomly divided into 4 groups after 2 weeks (n = 5 per group). On day 20, the mice were orally administered PBS, NAP (5 mg/kg), TMZ (10 mg/kg), or TMZ (10 mg/kg) plus NAP (5 mg/kg) twice a week. Treatment was performed for 2 weeks. Isoflurane was used for anesthesia. Mice were imaged for 10 min after intraperitoneal injection of D-luciferin (250 mg/kg) for bioluminescence. The signal intensity of tumor cells was quantified within a head region using Living Image software (PerkinElmer, MA, USA).

Zhong C., Tao B., Chen Y., Guo Z., Yang X., Peng L., Xia X, & Chen L. (2020). B7-H3 Regulates Glioma Growth and Cell Invasion Through a JAK2/STAT3/Slug-Dependent Signaling Pathway. OncoTargets and therapy, 13, 2215-2224.

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Silencing Noggin in Mouse Hippocampus

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Direct hippocampal injections of lentivirus expressing a shRNA against noggin (Bond et al., 2014 (link); Brooker et al., 2017 (link)) were performed using a mouse stereotaxic instrument (Stoelting Co), Quintessential Stereotaxic Injector (Stoelting Co), and a Hamilton microsyringe (5 μl, NEUROS Model, 33 g, Blunt). Mice were anesthetized via inhalation of isoflurane. Two small craniotomies were performed over the hippocampus of each hemisphere (coordinates relative to bregma: 2 mm posterior, 1.5 mm lateral, 1.9 mm ventral) and 2 μl of virus was injected bilaterally into the DG at a rate of 0.5 μl/min. Two weeks postinjection, levels of noggin protein were analyzed, and any mice lacking successful viral infection in the DG were excluded from the study.

Bonds J.A., Tunc-Ozcan E., Dunlop S.R., Rawat R., Peng C.Y, & Kessler J.A. (2023). Why Some Mice Are Smarter than Others: The Impact of Bone Morphogenetic Protein Signaling on Cognition. eNeuro, 10(1), ENEURO.0213-22.2022.

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Orthotopic Glioblastoma Xenograft in Mice

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Mice were anesthetized by intraperitoneal injection of 100 mg/kg body weight (bw) ketamine and 10mg/kg bw xylazine. Mice were then immobilized in a mouse stereotaxic instrument (Stoelting, Wood Dale, IL) and a 1 cm incision was made in the midline of the scalp to expose the sagittal suture and bregma of the skull. A burr hole of 1mm in diameter was made at a position of 2mm anterior and 2mm lateral right to the bregma. A Hamilton syringe containing MGPP3 cells was inserted 2mm deep from the skull surface. 50,000 cells in 1 μL DMEM medium were injected at 0.2 μL/min into the brains.

Wei H.J., Upadhyayula P.S., Pouliopoulos A.N., Englander Z.K., Zhang X., Jan C.I., Guo J., Mela A., Zhang Z., Wang T.J., Bruce J.N., Canoll P.D., Feldstein N.A., Zacharoulis S., Konofagou E.E, & Wu C.C. (2020). Focused Ultrasound-Mediated Blood-Brain Barrier Opening Increases Delivery and Efficacy of Etoposide for Glioblastoma Treatment. International journal of radiation oncology, biology, physics, 110(2), 539-550.

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