Carrier free sodium 35s sulfate

Ritodrine was a product of Santa Cruz Biotechnology Inc. (Dallas, TX). Adenosine 5′-triphosphate (ATP), 3′-phosphoadenosine-5′-phosphosulfate (PAPS), N-2-hydroxylpiperazine-N′-2-ethanesulfonic acid (HEPES), dimethyl sulfoxide (DMSO), Trizma base, dithiothreitol (DTT), and silica gel thin-layer chromatography (TLC) plates were from Sigma Chemical Company (St. Louis, MO, USA). Ultrafree-MC 5000 NMWL filter units were products of Millipore (Bedford, MA, USA). Carrier-free sodium [³⁵S]sulfate was a product of Perkin-Elmer (Waltham, MA, USA). Ecolume scintillation cocktail was purchased from MP Biomedicals, LLC. (Irvine, CA, USA). Recombinant human bifunctional ATP sulfurylase/adenosine 5′-phosphosulfate kinase was prepared as previously described (Yanagisawa et al., 1998 (link)). EX Taq DNA polymerase was a product of Takara Bio (Mountain View, CA, USA). Protein molecular weight markers were from New England Biolabs, Inc. (Ipswich, MA, USA). Oligonucleotide primers were synthesized by MWG Biotech (Huntsville, AL, USA). X-ray films were purchased from BioExpress (Kaysville, UT, USA). All other chemicals were of the highest grade commercially available.

Morphine, hydromorphone, oxymorphone, butorphanol, levorphanol, nalbuphine, nalorphine, naltrexone, 6β-naltrexol, buprenorphine, pentazocine, and naloxone, were purchased from Cerilliant. Adenosine 5’-triphosphate (ATP), sodium dodecyl sulfate (SDS), dithiothreitol (DTT), dimethyl sulfoxide (DMSO), 3-(N-morpholino)propanesulfonic acid (Mops), Trizma base (Tris), isopropyl-β-D-thiogalactopyranoside (IPTG), and silica gel thin-layer chromatography (TLC) plates were from Sigma Chemical Company. Polyethyleneimine (PEI) cellulose TLC plates were from Brinkmann. Ecolume scintillation cocktail was purchased from MP Biomedical. Carrier-free sodium [³⁵S]sulfate was from PerkinElmer. HepG2 human hepatoma cell line (ATCC HB-8065) was obtained from American Type Culture Collection. Pooled human lung S9 fraction, liver cytosol, small intestine (duodenum and jejunum) S9 fraction, and kidney S9 fraction were purchased from XenoTech, LLC. All other chemicals were of the highest grade commercially available.

Dehydroepiandrosterone (DHEA), tibolone, dithiothreitol (DTT), dimethylsulfoxide (DMSO), 3-(N-morpholino) propanesulfonic acid (MOPS), and isopropyl-1-thio-β-D-galactopyranoside (IPTG) were products of Sigma Chemical Company (St. Louis, MO USA). Carrier-free sodium [³⁵S]sulfate was a product of Perkin-Elmer (Waltham, MA USA). Ecolume scintillation cocktail was purchased from MP Biomedical (Solon, OH USA). Cellulose thin-layer chromatography (TLC) plates were from EMD chemicals (Gibbstown, NJ USA). Recombinant human bifunctional ATP sulfurylase/adenosine 5′-phosphosulfate kinase was prepared as previously described [21 (link)]. EX Taq DNA polymerase was a product of Takara Bio (Mountain View, CA USA). Protein molecular weight markers were from New England Biolabs, Inc. (Ipswich, MA USA). Oligonucleotide primers were synthesized by MWG Biotech (Louisville, KY USA). All other reagents were of the highest grades commercially available.