Antibodies directed against A20 (5630; Cell Signaling), ABIN-1 (HPA037893; Sigma Aldrich), c-IAP1 (ALX-803-335; Enzo and 4952; Cell Signaling), caspase 8 (9496, 4790; Cell Signaling and 804–429, Enzo), CC8 (8592, Cell Signaling), RIPK1 (3493; Cell Signaling), caspase 3 (9662; Cell Signaling), CC3 (9661; Cell Signaling), PARP (9532 and 9542; Cell Signaling), cFLIP (56343; Cell Signaling), mouse RIPK3 (95702; Cell Signaling), phospho-RIPK3 (T231/S232; ab205421; Abcam), phospho-RIPK1 (Ser166) rodent specific (31122; Cell Signaling), IKKβ (8943; Cell Signaling), phospho-IKKα/β (2697; Cell Signaling), and GAPDH (MAB374; Millipore) were used for Western blot as described below. Recombinant mouse TNF-α was purchased from R&D. Recombinant TWEAK (R&D) and TRAIL (Peprotech), were used at a final concentration of 250 ng/ml. FasL (R&D) was used at a final concentration of 40 ng/ml with anti-HA at 2.5 μg/ml (BioLegend). Nec1s (2263; BioVision) and emricasan (HY-10396; MedChemExpress) were used at 50 μM final concentration.
Recombinant tweak
Manufactured by R&D Systems
Sourced in United States
Recombinant TWEAK is a protein produced in a cell expression system. It is a member of the tumor necrosis factor (TNF) superfamily and is involved in various cellular processes. This product is intended for laboratory research use only.
Automatically generated - may contain errors
Lab products found in correlation
Nec 1, by Abcam (1 mentions)
Gapdh, by Merck Group (1 mentions)
C flip, by Cell Signaling Technology (1 mentions)
Phospho ikkα β, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Recombinant mouse tnf α, by R&D Systems (1 mentions)
Ripk1, by Cell Signaling Technology (1 mentions)
Anti ha, by BioLegend (1 mentions)
Recombinant human il 1β, by R&D Systems (1 mentions)
Dulbecco s modified eagle s medium dmem, by Cytiva (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Gentamycin, by Cytiva (1 mentions)
2 protocols using recombinant tweak
1
Antibodies for Apoptosis Signaling
2
Signaling Pathways Modulating Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant TWEAK, recombinant human IL-1β, and TNF-α were purchased from R&D Systems (Minneapolis, MN, USA). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), penicillin, and gentamycin were from Hyclone Laboratories (Logan, UT, USA). Anti-Fn14 mAb (ITEM4) was from eBioscience (SanDiego, CA, USA). Inhibitors of MAPK kinase 1 (PD98059), p38 MAPK (SB203580), JNK (SP600125), phosphoinositide 3-kinase (PI3K; LY294002), and NF-κB p65 (SC514) were purchased from Calbiochem (La Jolla, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!