V. parahaemolyticus strains were cultured in MLB at 37 °C for 45 mins without or with 100 µM TDC to induce T3SS2 expression. Cells were collected at 4000 × g for 10 mins, washed in 0.2 M phosphate buffer, and then fixed with 8% paraformaldehyde in 0.2 M phosphate buffer for 10 minutes at room temperature. After an additional wash with 0.2 M phosphate buffer, the cell pellets were resuspended in 0.1 M phosphate buffer with 4% paraformaldehyde. The samples were subsequently embedded in LR White resin, thin-sectioned, mounted on nickel-coated grids, and stained with the appropriate concentration of primary and nano-gold-conjugated secondary antibodies for transmission electron microscopy. All imaging was done on the JEOL 1400 Plus Transmission Electron Microscope equipped with a BIOSPR CCD camera at the University of Texas Southwestern Medical Center EM core facility.
Ccd camera
Manufactured by JEOL
Sourced in Japan
The CCD camera is a versatile imaging device designed for use in various laboratory applications. It captures and converts light signals into digital image data for analysis and documentation purposes. The CCD camera's core function is to provide high-quality, detailed images of specimens or samples under examination.
Automatically generated - may contain errors
Lab products found in correlation
K alpha, by Thermo Fisher Scientific (2 mentions)
1400 plus transmission electron microscope, by JEOL (1 mentions)
Jem 1011 electron microscope, by JEOL (1 mentions)
Jem 1400 electron microscope, by JEOL (1 mentions)
D max 2500v pc, by Rigaku (1 mentions)
Jem 2100f, by JEOL (1 mentions)
D 5000 powder x ray diffractometer, by Siemens (1 mentions)
Via reflex raman microscope, by Renishaw (1 mentions)
Jem 1010 instrument, by JEOL (1 mentions)
Jsm 6700f, by JEOL (1 mentions)
6 protocols using ccd camera
1
Visualizing Vibrio parahaemolyticus T3SS2
2
Electron Microscopy Protocol for Cellular Ultrastructure
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For conventional ultrathin section electron microscopy, cells cultured on coverslips were fixed with a mixture of 2% formaldehyde and 2.5% glutaraldehyde in 0.1 M HEPES buffer (pH 7.4) for more than 2 h at room temperature, and post-fixed with a mixture of 1% osmium tetroxide and 0.1% potassium ferrocyanide in 0.1 M sodium cacodylate buffer (pH 7.4)71 (link). For delineation of isolation membranes and IMATs, cells were fixed for 40 min at 4 °C with a glutaraldehyde–osmium tetroxide mixture using a slight modification of the published methods34 (link),72 (link). The fixative was prepared by mixing one volume of solution A (2.5% glutaraldehyde, 0.1 M sodium cacodylate buffer [pH 7.4]) and two volumes of solution B (1% osmium tetroxide, 3 mg ml–1 potassium ferrocyanide, 0.1 M sodium cacodylate buffer [pH 7.4]) immediately before use. All the samples were dehydrated, and embedded in epoxy resin. Ultrathin sections were observed using a JEOL JEM1011 electron microscope operated at 100 kV. Digital images were captured using a CCD camera (Gatan).
3
Transmission Electron Microscopy Imaging
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TEM were conducted on a JEM-1400 electron microscope equipped with a CCD camera (JEOL Japan, Tokyo, Japan). Samples were prepared by drying diluted droplets on carbon-film coated copper grids at room temperature and the CCD camera was inserted in for further observations.
4
Comprehensive Characterization of RuO2@C
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The morphology and size of RuO2@C were investigated by using transmission electron microscopy (TEM; JEOL, JEM-2100F; accelerating voltage at 200 kV with Gatan CCD camera). The functional groups of RuO2@C annealed at different temperatures were analysed by X-ray photoelectron spectroscopy (XPS; Thermo Fisher, K-alpha). The crystallography of RuO2 was investigated by high power X-ray diffractometer (XRD; Rigaku, D/MAX 2500 V/PC).
5
Structural Characterization of Materials
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X-ray diffraction (XRD) patterns were collected using a Siemens D-5000 powder X-ray diffractometer operated in Bragg Brentano geometry using Ni-filtered Cu Kα radiation (λ = 0.1541 nm). Data were recorded in the 2θ range 10–80° with an angular step size of 0.026° and a counting time of 1 s per step. The collected data were refined using the Le Bail method by means of the software Rietica [34 (link),35 (link)]. Raman spectra were collected from powder samples onto a glass slide as substrate, with a Renishaw in Via Reflex Raman microscope (Renishaw, Gloucestershire, UK). Experiments were conducted at room temperature using 532 and 633 nm excitation wavelengths. Field emission scanning electron microscopy (FESEM) was conducted on a JEOL JSM-6700 F. Samples for transmission electron microscopy (TEM) studies were prepared by dropping a diluted suspension of the samples onto ultra-thin carbon-coated copper grids. Imaging was performed on a JEOL JEM 1010 instrument operated at 100 kV and equipped with a CCD camera (JEOL, Tokyo, Japan).
6
Unbiased Quantitative Analysis of Excitatory Synapses
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