This study used female mice aged 8 to 12 weeks old. To induce development of colitis, high–molecular-weight, colitis-grade DSS with an average molecular weight of 36,000 to 50,000 (MP Biomedicals, Santa Ana, CA, USA) was added to the drinking water for 7 days at a concentration of either 1 or 2% [32 (link)]. Control mice were received plain drinking water without DSS. The severity of colitis was assessed daily by scoring body weight loss, stool consistency, and occult blood in the stool on a scale ranging from 0 (normal) to 4 (severe) and calculating the total disease activity index (DAI) score as the sum of these 3 scores (maximum score: 12), in accordance with a previous report [33 (link)]. To evaluate anemia, we measured the number of erythrocytes and concentration of hemoglobin (HGB) and hematocrit (HCT) in peripheral blood by Celltac alpha (Nihon Kohden, Tokyo, Japan).
Celltac alpha
Manufactured by Nihon Kohden
Sourced in Japan
The Celltac Alpha is a hematology analyzer designed for in-vitro diagnostic use. It is capable of performing complete blood count (CBC) analysis, including the measurement of red blood cells, white blood cells, and platelets. The device utilizes optical and impedance-based methods to obtain accurate and reliable results.
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Lab products found in correlation
11 protocols using celltac alpha
1
Induction and Evaluation of Murine Colitis
2
Analysis of Blood Components in Phd2 cKO Mice
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Whole blood was collected 6 weeks after tamoxifen administration from the control and Phd2 cKO mice via the buccal vein under anaesthesia. All blood components were analysed using Celltac Alpha (Nihon Kohden, Tokyo, Japan). After centrifugation, the supernatant was stored at −20 °C until further examination. Plasma erythropoietin (EPO), vascular endothelial growth factor (VEGF) and nitric oxide (NO) metabolite levels were measured using enzyme‐linked immunosorbent assay kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocols. NO is a gaseous free radical with a short half‐life of a few seconds or less in vivo. Therefore, the levels of more stable NO metabolites, nitrite (NO 2 − NO 3 − 1992 ).
3
Acute Hemolytic Anemia Induction in Mice
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To generate acute hemolytic anemia, mice were intraperitoneally injected with 40 mg/kg per mice of PHZ (114715; Sigma-Aldrich). As an alternative method to generate acute anemic stress, 400–500 µl of peripheral blood was obtained from the submental vein of the mice (8–12 wk old) for three consecutive days. Peripheral blood was obtained from the superficial temporal vein of mice for total blood count, collected in K2EDTA tubes (BD), and analyzed via the Celltac Alpha veterinary hematology analyzer (Nihon Kohden).
4
Comprehensive Blood Biomarker Analysis
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Blood samples were collected with anticoagulant (K3EDTA) and tested with an automated haematology analyser, Celltac Alpha (Nihon Kohden, Tokio, Japan). The following parameters were measured: white blood cell count, eosinophil count and percentage, red blood cell count, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, lymphocyte count and percentage, monocyte count and percentage, granulocyte count and percentage, red cell distribution width, procalcitonin test, mean platelet volume, platelet distribution width, and platelet count.
Sera prepared from the blood samples were analysed with an automated biochemistry analyser (Cobas 6000 C501; Roche, Basel, Switzerland). The following biochemical parameters were measured: total protein, albumin, gamma-glutamyl transferase, bilirubin, cholesterol, alanine aminotransferase, glucose, calcium, phosphorus, creatinine, and blood urea nitrogen.
Sera prepared from the blood samples were analysed with an automated biochemistry analyser (Cobas 6000 C501; Roche, Basel, Switzerland). The following biochemical parameters were measured: total protein, albumin, gamma-glutamyl transferase, bilirubin, cholesterol, alanine aminotransferase, glucose, calcium, phosphorus, creatinine, and blood urea nitrogen.
5
Assessing Toxicity Through Hematological and Biochemical Analysis
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To assess the toxicity, body weight of each mouse was measured every 3-4 days. At 27 days after the first administration, all mice were euthanized. Their blood samples were collected through cardiac puncture, and the hematological parameters and biochemical parameters relevant to hepatic and renal functions were analyzed. We used an automated hematology analyzer, Celltac Alpha (Nihon Kohden, Tokyo, Japan), for hematological and automated clinical chemistry analyzer, DRI-CHEM 7000V (Fujifilm, Tokyo, Japan), for biochemical tests.
6
Bovine Jugular Vein Blood Sampling
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Blood of the measurable cows by TE was taken from the jugular vein or coccygeal vein. Blood samples were collected in a plain vacuum blood collection tube (Terumo Corp., Tokyo, Japan) for
biochemical tests and in an ethylenediaminetetraacetic acid disodium salt vacuum blood collection tube (Terumo Corp.) for complete blood cell count (CBC) and total protein (TP) measurement.
The plain vacuum blood collection tube was coagulated at room temperature, centrifuged at 1,700 × g for 15 min at 4°C and stored at −20°C, until used for biochemical tests.
CBC was measured using the Celltac Alpha (Nihon Kohden Corporation, Tokyo, Japan), and TP was measured using a refractometer. Blood biochemical tests were performed by Fujifilm Monolith
(Tokyo, Japan) and measured albumin (ALB), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (γ-GTP), total cholesterol (T-Chol), and triglycerides (TG).
biochemical tests and in an ethylenediaminetetraacetic acid disodium salt vacuum blood collection tube (Terumo Corp.) for complete blood cell count (CBC) and total protein (TP) measurement.
The plain vacuum blood collection tube was coagulated at room temperature, centrifuged at 1,700 × g for 15 min at 4°C and stored at −20°C, until used for biochemical tests.
CBC was measured using the Celltac Alpha (Nihon Kohden Corporation, Tokyo, Japan), and TP was measured using a refractometer. Blood biochemical tests were performed by Fujifilm Monolith
(Tokyo, Japan) and measured albumin (ALB), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (γ-GTP), total cholesterol (T-Chol), and triglycerides (TG).
7
Accelerating Neutrophil Recovery in Rats
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The effect of ABD-GCSF in acceleration of neutrophil count was investigated in normal Sprague Dawley male rats (6–7 weeks, 250–300 g weight). The research protocols and animal studies were approved by the Ethics Committee of Pasteur Institute of Iran (IR.PII.REC.1399.013) and followed ARRIVE reporting guidelines62 (link). Animals were adopted to the conditions of light and humidity for one week and were randomly divided into five groups (five rats/group). The animals except control group (Group 1) intraperitoneally (i.p) received 100 mg/kg of cyclophosphamide (CPA) for induction of neutropenia on day zero12 (link),63 (link). On day 1, the rats in Groups 2 to 4 subcutaneously (s.c) received 100 µg/kg of Filgrastim, PEG-Filgrastim, or ABD-GCSF, respectively and the 5th group received PBS alone as control12 (link),30 (link),64 (link). Blood samples were collected from tail vein in non-vacuumed K2EDTA Nex tubes (Nexamo Technoplast, India) according to the designed time schedule on 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 11 days post GCSF injection. Complete blood counting (CBC) of neutrophils, lymphocytes, eosinophils, monocytes, red and white blood cells was done by hematology analyzer Celltac alpha (Nihon Kohden, Japan).
8
Physiological Response of Dairy Cows to Feeding
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Blood samples were collected from the jugular vein 5 h after feeding on the last day of each period (with heparin as an anticoagulant) to obtain whole blood, plasma, and PBMC. Each heparinized blood sample was divided into 3 portions. The first portion was used to measure blood hematology: red blood cell count (RBC), hemoglobin and hematocrit levels, mean corpuscular volume (MCV), mean corpuscular hemoglobin level, mean corpuscular hemoglobin concentration, platelet and white blood cell (WBC) counts, and differential count of WBC by automatic blood analyzer (Nihon Kohden, Celltac Alpha, Tokyo, Japan). The second portion was centrifuged at 3,000 × g for 15 min at 4°C to obtain plasma. Plasma samples were stored at -30°C until biochemical analysis. The third portion was used to obtain PBMC.
Milk yield was automatically recorded at each milking, with data used only from the last 2 d of each period. Milk was sampled according to the milk yield of each cow during the last 2 d of each period. Samples of milk were stored at -30°C until analysis of milk composition.
Milk yield was automatically recorded at each milking, with data used only from the last 2 d of each period. Milk was sampled according to the milk yield of each cow during the last 2 d of each period. Samples of milk were stored at -30°C until analysis of milk composition.
9
Plasma Gut Hormone Analysis Protocol
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Blood samples were immediately transferred into disodium EDTA-treated tubes to measure plasma gut hormones, glucose, insulin and IL-6. Aprotinin, a potent protease inhibitor, was added to samples at a concentration of 500 kIU/mL for the measurement of AG. Test tubes were then centrifuged at 1308 g at 4°C for 15 min immediately after collection, and plasma samples were stored at −80°C for later analyses. Hematocrit was measured using Celltac alpha (Nihon Kohden Inc., Tokyo, Japan). Glucose was measured using the enzymatic reference method with hexokinase. Insulin was assessed by the fully automated chemiluminescence method (CLIA). Plasma IL-6 (Human IL-6 Immunoassay kit, R&D Systems), GLP-1 (GLP-1 (7–36) amide) (Human GLP-1 EIA kit, Yanaihara Institute Inc., Shizuoka, Japan), PYY (Human PYY EIA kit, Yanaihara Institute Inc.) and AG (Active Ghrelin ELISA kit, Mitsubishi Kagaku Iatron Inc., Tokyo, Japan) concentrations were assessed by ELISA. ELISA for PYY quantified the total amount of PYY1–36 and PYY3–36. The coefficients of variation values of IL-6, GLP-1, PYY and AG were 4.2, 8.6, 5.2 and 8.4%, respectively. All sample measurements were performed in duplicate according to the manufacturers’ instructions.
10
Mouse Blood Analysis After Treatment
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After the final day of treatment, blood was collected from three mice in each treatment group. After euthanasia, blood was obtained by puncture axillary artery and vein. The blood volume collected from each mouse was 500 μl. Death was verified as described above. The numbers of white blood cells (WBC), red blood cells (RBC), and platelet (Plt) and hemoglobin (Hb) were measured using an automatic blood cell counter (Celltac alpha; Nihon Kohden, Tokyo, Japan). Serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and creatinine (Cre) were measured using BiOLiS 24i (Tokyo Boeki Medisys Inc., Tokyo, Japan). Coagulated samples were excluded from the a Line 7nalysis.
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