Hc fluotar l 25 0.95 visir water objective

Manufactured by Leica

The HC FLUOTAR L 25×/0.95 VISIR water objective is a high-numerical aperture objective lens designed for microscopy applications. It provides a magnification of 25× and a numerical aperture of 0.95, enabling high-resolution imaging and light collection. The lens is optimized for use with water-based samples.

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Lab products found in correlation

Hc pl apo 63 x 1.40 cs2 oil objective, by Leica (2 mentions) Tcs sp8, by Leica (1 mentions) Tcs sp8 inverted, by Leica (1 mentions)

2 protocols using hc fluotar l 25 0.95 visir water objective

Confocal Microscopy Imaging Protocol

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All imaging was performed on a white-light Leica TCS SP8 inverted confocal microscope using either an HC FLUOTAR L 25×/0.95 VISIR water objective (Leica) or an HC PL APO 63 x/1.40 CS2 oil objective (Leica). All images were taken at 1024x1,024 pixel resolution, and any tile scan images were merged using Leica software.

Cincotta S.A., Richardson N., Foecke M.H, & Laird D.J. (2024). Differential susceptibility of male and female germ cells to glucocorticoid-mediated signaling. eLife, 12, RP90164.

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Confocal Microscopy of Biological Samples

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All imaging was performed on a white-light Leica TCS SP8 inverted confocal microscope using either an HC FLUOTAR L 25×/0.95 VISIR water objective (Leica) or an HC PL APO 63x/1.40 CS2 oil objective (Leica). All images were taken at 1024 x 1024 pixel resolution, and any tile scan images were merged using Leica software.

Cincotta S.A., Richardson N., Foecke M.H, & Laird D.J. (2023). Differential susceptibility of male and female germ cells to glucocorticoid-mediated signaling. bioRxiv.

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