Cylindrical lens

Manufactured by Thorlabs

Sourced in United States

A cylindrical lens is an optical component that focuses light in one dimension while leaving the other dimension unchanged. It has a cylindrical surface that refracts light differently in the two orthogonal directions, allowing for the creation of line-focused beams or the correction of astigmatism.

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Lab products found in correlation

L808p1000mm, by Thorlabs (1 mentions) Ixonem 897, by Oxford Instruments (1 mentions) Phoxx 642, by Toptica (1 mentions) 488 nm laser, by Toptica (1 mentions)

3 protocols using cylindrical lens

Side Scattering Imaging Setup

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The LVM setup was composed of a 1 W, 808 nm multimode diode laser (L808P1000MM, Thorlabs Inc., Newton, NJ, USA) with a collimation lens and a cylindrical lens (Thorlabs Inc., Newton, NJ, USA) to focus the laser beam into a light slab through the sample. Side scattering images were recorded by a CMOS camera (Flea3 FL3-U3-13S2M, Point Gray Research Inc., Richmond, BC, Canada) for 30 s at 800 × 600-pixel resolution, 40 fps, and 2× magnification through a variable zoom lens (NAVITAR 12×, Navitar, New York, NY, USA) placed at a 90° angle to the laser light beam. The image volume (1.81 μL) was determined by the size of the light slab that illuminated the sample and by the viewing size and focal depth of the optics (1.85 mm × 1.4 mm × 0.7 mm).

Iriya R., Braswell B., Mo M., Zhang F., Haydel S.E, & Wang S. (2024). Deep Learning-Based Culture-Free Bacteria Detection in Urine Using Large-Volume Microscopy. Biosensors, 14(2), 89.

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Light-Based Imaging of Microscopic Samples

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LVM consists of a 100 mW, 637 nm fiber-coupled diode laser (OBIS 637-100FP, Coherent Inc., USA) with a collimation lens and a cylindrical lens (Thorlabs Inc, USA) to focus the laser beam into a light slab through the sample. Wide-view and deep field depth side scattering images were recorded by a CMOS camera (Flea3 FL3-U3-13S2M, Point Grey Research Inc., Canada) at 80 fps through a 12X variable zoom lens (NAVITAR 12X, Navitar, USA) placed at a 90-degree angle to the laser light beam. The image volume was determined by the size of the light slab that illuminated the sample and by the viewing size and focal depth of the optics. For the experiments described in this study, the viewing area of 1.85 mm x 1.4 mm x 0.7 mm was equivalent to 1.81 μL at 2.5X magnifying power. The LVM was enclosed in a thermally isolated housing with a controlled temperature (37°C).

Mo M., Yang Y., Zhang F., Jing W., Iriya R., Popovich J., Wang S., Grys T., Haydel S.E, & Tao N. (2019). Rapid Antimicrobial Susceptibility Testing of Patient Urine Samples using Large Volume Free-Solution Light Scattering Microscopy. Analytical chemistry, 91(15), 10164-10171.

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High-resolution 3D Fluorescence Microscopy

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Images were acquired using a modified Olympus
IX81 inverted epifluorescent
microscope with an oil-immersion objective (PlanApo N, 60×, NA
1.42, Olympus, Vienna, Austria). Samples were mounted on a XYZ piezo
stage (PI Mars; P-562, Physical Instruments) which has nanometer accuracy,
combined with a coarse mechanical stage with a travel range of 1 cm
× 1 cm (Hybrid, JPK Instruments, Berlin, Germany). A tube lens
with an additional magnification of 1.6 was used to achieve a final
imaging magnification of 96 (corresponding to a pixel size of 167
nm). ECs were illuminated with a 642 nm laser diode (Omicron-laserage
Laserprodukte GmbH, Phoxx 642, Rodgau-Dudenhofen, Germany) and a 488
nm laser (Toptica Photonics, Germany). Signals were collected using
an Andor iXonEM+ 897 (back-illuminated) EMCCD camera (16 μm
pixel size). The following filter sets were used: dichroic filter
(ZT405/488/561/640rpc, Chroma, Germany), emission filter (446/523/600/677
nm BrightLine quad-band band-pass filter, Semrock, Rochester, NY,
USA), and additional emission filters: ET 700/75 M, Chroma Technology
GmbH, Olching, Germany; ET 525/50 M, Chroma Technology GmbH, Olching,
Germany. For 3D measurements, a cylindrical lens (f = 500 mm; Thorlabs, Newton, USA) was placed into the detection path
of the microscope.

Buchroithner B., Mayr S., Hauser F., Priglinger E., Stangl H., Santa-Maria A.R., Deli M.A., Der A., Klar T.A., Axmann M., Sivun D., Mairhofer M, & Jacak J. (2021). Dual Channel Microfluidics for Mimicking the Blood–Brain Barrier. ACS Nano, 15(2), 2984-2993.

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