Sarcoma 180 cancer cells were cultured in RPMI 1640 media (Gibco) containing 10% FBS (Gibco) and 1% penicillin/streptomycin (Invitrogen) and incubated at 5% CO2, 37 °C. An MTT cell proliferation assay kit (Promega) was used to evaluate the cytotoxicity of Fe3O4@ALG and Fe3O4/Cur@ALG. A total of 5 × 106 cells were exposed to Fe3O4 concentrations ranging from 0.01 to 1000 µg/mL for 48 h. The samples were measured using laminator spectrometers at a wavelength of 570 nm. The IC50 (the half maximal inhibitory concentration) value was calculated according to the guidelines of the MTT assay kit.
Mtt cell proliferation assay kit
Manufactured by Promega
Sourced in United States
The MTT cell proliferation assay kit is a colorimetric assay used to measure the metabolic activity of cells. The assay is based on the reduction of the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by the mitochondrial enzymes of viable cells, resulting in the formation of purple formazan crystals. The amount of formazan produced is directly proportional to the number of living cells and can be quantified by measuring the absorbance at a specific wavelength.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Live dead viability cytotoxicity kit, by Promega (1 mentions)
Myosin heavy chain, by Abcam (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
C2c12 mouse myoblast cells, by American Type Culture Collection (1 mentions)
Synergy h4, by Bioteck (1 mentions)
4 protocols using mtt cell proliferation assay kit
1
Cytotoxicity Evaluation of Magnetic Nanoparticles
2
Peptide-Mediated Myoblast Proliferation
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Two tetrameric self-assembling peptides CH-01 and CH-02 were custom-synthesized in our Laboratory for Nanomedicine. Mouse myoblast cells (C2C12) were obtained from ATCC, USA. The following materials were ordered from Gibco, USA: Dulbecco’s modified eagle medium (DMEM), fetal bovine serum (FBS), heat-inactivated horse serum, Dulbecco’s phosphate-buffered saline (PBS) solution, and penicillin-streptomycin (P/S) antibiotics. An 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay kit and a LIVE/DEAD Viability/Cytotoxicity kit were purchased from Promega, USA. Immunostaining antibody myosin heavy chain (MHC) was purchased from Abcam. Cell culture flasks and 96-well plates were ordered from Corning, USA.
3
Cell Viability Assay for ADan Oligomers
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HEK P301L cells were plated at 20,000 cells per well in 96-well plates with 1 μg/mL Dox for 24 h. Then the cells were treated with 2.5 μM ADan oligomers, monomers, or PBS. After incubation for 4 h at 37 °C, cell viability was assayed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay kit (Promega) according to the manufacturer’s specifications. The MTT assay is a colorimetric assay for assessing cell metabolic activity, which reflects the number of viable cells. All measurements were made in six replicates.
4
Cell Viability Assay for ANC Treatment
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Cell viability was determined with the MTT cell proliferation assay kit (Promega, Madison, WI, USA) after incubation with ANCs. Respectively, 15,000 U87 cells and 20,000 J774 cells were plated in each well of a 96-well plate. After 24 h, dOA-ANCs and BSA-ANCs were added to the cell medium at different iron concentrations. After 48 h of incubation, the MTT assay was performed according to the manufacturer’s instructions, and the absorbance was measured with a microplate reader (SynergyH4, Bioteck, Winooski, VT, USA).
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