Iopamidol was quantified by using a hybrid quadrupole-orbitrap mass spectrometer (
Q Exactive, Thermo Fisher Scientific Inc., Waltham, MA, USA) coupled with liquid chromatography (
UltiMate 3000 LC systems, Thermo Fischer Scientific Inc.). To investigate the TPs derived from the iopamidol-containing solution during ozonation and ozonation-chlorination, their accurate masses were similarly determined by using the hybrid quadrupole-orbitrap mass spectrometer coupled with liquid chromatography. Two low-molecular-weight iodic TPs (iodoacetic acid and iodoform) were quantified by using the LC-MS/MS system (LC,
UltiMate 3000 LC systems; MS/MS,
Q Exactive, Thermo Fisher Scientific Inc.) and a GC-MS system (
QP2010 Plus, Shimadzu Corporation, Kyoto, Japan) equipped with a capillary column (
DB-1ms UI, Agilent Technologies, Palo Alto, CA, USA; length, 30 m; internal diameter, 0.25 mm; thickness, 0.25 µm), respectively. Details regarding measurements are described in Supplementary content.
Matsushita T., Hashizuka M., Kuriyama T., Matsui Y, & Shirasaki N. (2016). Use of orbitrap-MS/MS and QSAR analyses to estimate mutagenic transformation products of iopamidol generated during ozonation and chlorination. Chemosphere, 148.