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Db 1 ms ui

Manufactured by Agilent Technologies
Sourced in Canada, Japan

The DB-1 MS UI is a capillary gas chromatography column designed for use in mass spectrometry applications. It features a 100% dimethylpolysiloxane stationary phase and is intended for the separation of a wide range of volatile and semi-volatile compounds.

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2 protocols using db 1 ms ui

1

GC×GC/ToF-MS Analysis Protocol

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Analysis was conducted in electron
ionization mode using a GC×GC/ToF-MS Pegasus 4D (LECO, St. Joseph,
MI) equipped with an Agilent 7890B gas chromatograph (Palo Alto, CA).
The columns used were the DB-1 MS UI (25 m × 0.25 mm × 0.25
μm; Agilent Technologies) and Rxi-17 Sil MS column (1.2 m ×
0.25 mm × 0.25 μm; Restek) in the first and second dimension,
respectively (Table S3).
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2

Quantifying Iopamidol and Its Transformation Products

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Iopamidol was quantified by using a hybrid quadrupole-orbitrap mass spectrometer (Q Exactive, Thermo Fisher Scientific Inc., Waltham, MA, USA) coupled with liquid chromatography (UltiMate 3000 LC systems, Thermo Fischer Scientific Inc.). To investigate the TPs derived from the iopamidol-containing solution during ozonation and ozonation-chlorination, their accurate masses were similarly determined by using the hybrid quadrupole-orbitrap mass spectrometer coupled with liquid chromatography. Two low-molecular-weight iodic TPs (iodoacetic acid and iodoform) were quantified by using the LC-MS/MS system (LC, UltiMate 3000 LC systems; MS/MS, Q Exactive, Thermo Fisher Scientific Inc.) and a GC-MS system (QP2010 Plus, Shimadzu Corporation, Kyoto, Japan) equipped with a capillary column (DB-1ms UI, Agilent Technologies, Palo Alto, CA, USA; length, 30 m; internal diameter, 0.25 mm; thickness, 0.25 µm), respectively. Details regarding measurements are described in Supplementary content.
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