Frozen sections from mouse tumor tissues (thickness 5 µm) were blocked with 5% bovine serum albumin for 1 h at 37 °C and stained with anti-mouse CD3 antibody (Proteintech, Wuhan, China) or FITC-conjugated anti-mouse CD8 antibody (Biolegend, San Diego, CA, USA) overnight at 4 °C. For CD3 staining, the sections were incubated with Alexa Fluor® 594-labeled goat anti-rabbit secondary antibody (Abcam) staining for 45 min at room temperature. Next, the slides were incubated with DAPI at room temperature for 5 min. All fluorescence images were taken using a Nikon Eclipse/ NI-U microscope. The CD3+ T and CD8+ T ratio was calculated in three random view fields.
Fitc conjugated anti mouse cd8 antibody
Manufactured by BioLegend
Sourced in China
The FITC-conjugated anti-mouse CD8 antibody is a flow cytometry reagent used to detect and quantify CD8+ T cells in mouse samples. The antibody specifically binds to the CD8 surface marker, which is expressed on cytotoxic T cells. The FITC fluorescent label allows for the visualization and analysis of CD8+ cells using flow cytometry techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc conjugated anti mouse cd8 antibody
1
Immunofluorescence Analysis of T Cell Subsets
2
Decidual and Spleen Cell Phenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The expression levels of cell surface molecules CD8, Tim-3, and PD-1 on decidual and spleen cells from normal pregnant and abortion-prone mice were evaluated using flow cytometry. FITC-conjugated anti-mouse CD8 antibody
(BioLegend), PE-conjugated anti-mouse Tim-3 antibody (BioLegend), and PE-cy7-conjugated anti-mouse PD-1 antibody (BioLegend) were used. The production of intracellular cytokines (TNF-α, IFN-γ, IL-4, and IL-10) by
Tim-3+PD-1+, Tim-3+PD-1–, Tim-3–PD-1+, and Tim-3–PD-1– decidual and spleen CD8+ cells from normal pregnant and abortion-prone
mice were also evaluated using flow cytometry. Freshly isolated DICs and splenocytes were treated with brefeldin A (10 μg/ml), phorbol 12-myrstate 13-acetate (PMA) (50 ng/ml), and ionomycin (1 μg/ml) for 4 h, then the cells were
harvested and analyzed by flow cytometry. APC-conjugated anti-mouse TNF-α or IL-10 antibodies (BioLegend) and Brilliant Violet 421-conjugated anti-mouse IFN-γ or IL-4 antibodies (BioLegend) were used. For intracellular staining,
cells were fixed and permeabilized using the Fix/Perm kit (BioLegend). Flow cytometry was performed on a Beckman-Coulter CyAN ADP cytometer and analyzed with FlowJo software (Tree Star, Ashland, OR, USA).
(BioLegend), PE-conjugated anti-mouse Tim-3 antibody (BioLegend), and PE-cy7-conjugated anti-mouse PD-1 antibody (BioLegend) were used. The production of intracellular cytokines (TNF-α, IFN-γ, IL-4, and IL-10) by
Tim-3+PD-1+, Tim-3+PD-1–, Tim-3–PD-1+, and Tim-3–PD-1– decidual and spleen CD8+ cells from normal pregnant and abortion-prone
mice were also evaluated using flow cytometry. Freshly isolated DICs and splenocytes were treated with brefeldin A (10 μg/ml), phorbol 12-myrstate 13-acetate (PMA) (50 ng/ml), and ionomycin (1 μg/ml) for 4 h, then the cells were
harvested and analyzed by flow cytometry. APC-conjugated anti-mouse TNF-α or IL-10 antibodies (BioLegend) and Brilliant Violet 421-conjugated anti-mouse IFN-γ or IL-4 antibodies (BioLegend) were used. For intracellular staining,
cells were fixed and permeabilized using the Fix/Perm kit (BioLegend). Flow cytometry was performed on a Beckman-Coulter CyAN ADP cytometer and analyzed with FlowJo software (Tree Star, Ashland, OR, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!