Xm10 fluorescence camera

Manufactured by Olympus

The XM10 Olympus fluorescence camera is a high-performance imaging device designed for use in various research and scientific applications. It is capable of capturing detailed fluorescence images with high sensitivity and resolution, making it suitable for a wide range of applications in the field of microscopy and imaging.

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XM10 camera (61 citations)

2 protocols using xm10 fluorescence camera

Cardiomyocyte Size Quantification

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Hearts were snap frozen in OCT, sectioned at 10μm, and stained with wheat germ agglutinin (WGA, Alexa 488) and DAPI (Alexa 405). Cardiomyocyte diameter was measured counting a minimum of 300 cells containing a central nucleus on 3 non-consecutive sections per animal. Cardiomyocyte cross-sectional area was measured 5um-thick heart sections stained with WGA (Alexa fluor 488). Images were taken using CellSens Dimension viewing software (Version 1.1.3, Olympus). The area was assessed on fibers with circular shapes in a blinded fashion by quantitative image analysis using CellSens Dimension software. The images were acquired with an XM10 Olympus fluorescence camera.

Pires K.M., Buffolo M., Schaaf C., Symons J.D., Cox J., Abel E.D., Selzman C.H, & Boudina S. (2017). Activation of IGF-1 Receptors and Akt signaling by systemic hyperinsulinemia contributes to cardiac hypertrophy but does not regulate cardiac autophagy in obese diabetic mice. Journal of molecular and cellular cardiology, 113, 39-50.

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Quantifying Macrophages and Thermogenic Activity

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Paraffin-embedded eWAT sections were stained with an anti-F4/80 antibody to detect macrophages in the adipose tissue. Uncoupling protein (UCP)-1 immunoreactivity was assessed in paraffin-embedded BAT sections (Supplementary Data). The images were acquired with an XM10 Olympus fluorescence camera. F4/80/DAPI and immunofluorescence staining was quantified based on color intensity using cellSens software. UCP-1/DAPI immunofluorescence staining was quantified based on color intensity using cellSens software in representative nonconsecutive fields per slide from two slides per animal in a blinded fashion.

Han Y.H., Buffolo M., Pires K.M., Pei S., Scherer P.E, & Boudina S. (2016). Adipocyte-Specific Deletion of Manganese Superoxide Dismutase Protects From Diet-Induced Obesity Through Increased Mitochondrial Uncoupling and Biogenesis. Diabetes, 65(9), 2639-2651.

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