Wtc 0200s

Samples were separated over a WTC-0200S (Wyatt Technologies) size exclusion column utilizing an Agilent 1200 HPLC to apply and maintain a 0.7 mL/min flowrate of 50 mM phosphate, pH 7.2 buffer containing 100 mM sodium chloride and 200 ppm sodium azide. Samples of 25 μL were injected onto the column and total run time was 30 min. Samples were detected using a UV – vis detector (Agilent), a Wyatt HELEOS Multi Angle Laser Light Scattering (MALS) detector, a quasi-elastic light scattering detector (QELS), and an Optilab rEX differential refractometer (Wyatt Technology Corporation). The number-average molecular weight, Mn, was calculated with Astra 5.3.14 software (Wyatt Technology Corporation) based on the molecular weight distribution.

Samples were separated over a WTC-0200S (Wyatt Technologies) size exclusion column utilizing an Agilent 1200 HPLC system at a flow rate 0.7 ml/min of MALS buffer (50 mM phosphate, 100 mM NaCl and 200 ppm NaN₃, pH 7.2). Sample volume of 25 µL per injection was loaded onto the column, and the column was run for 35 min. Eluted peaks were detected using a UV-Vis detector (Agilent), a Wyatt HELEOS multi-angle laser light scattering (MALS) detector, and an Optilab rEX differential refractometer (Wyatt Technology Corporation). The number-average particle molecular weight, Mn, was measured across FWHM of each peak with Astra 5.3.14 software (Wyatt Technology Corporation) using a previously calculated dn/dc value of 0.185 mL/g.

Samples were separated over a WTC-0200S (Wyatt Technologies) size exclusion column utilizing an Agilent 1200 HPLC system at a flow rate 0.7 ml min⁻¹ of MALS buffer (50 mM phosphate, 100 mM NaCl and 200 ppm NaN₃, pH 7.2). Sample volume of 25 μL per injection was loaded onto the column, and the column was run for 35 min. Eluted peaks were detected using a UV-Vis detector (Agilent), a Wyatt HELEOS multi-angle laser light scattering (MALS) detector, and an Optilab rEX differential refractometer (Wyatt Technology Corporation). The number-average particle molecular weight, Mn, was measured across FWHM of each peak with Astra 6.0.3.16 (Wyatt Technology Corporation) using a previously calculated dn/dc value of 0.185 mL g⁻¹.