Diabetes was induced by a single intraperitoneal injection of STZ (55 mg/kg) freshly prepared in 0.1 mol/L citrate buffer (pH 4.5).14 (link) Control rats were injected with the same volume of citrate buffer as vehicle. Forty-eight hours after the STZ injection, the fasting glucose levels were detected in a tail vein blood samples using ACCU-CHEK compact plus glucometer (Roche Diagnostics, Meylan, France). Rats with glucose level of 250 mg/dL or higher were considered diabetic.
Accu chek compact plus glucometer
Manufactured by Roche
Sourced in France, Switzerland, United States
The Accu-Chek Compact Plus is a glucometer designed for blood glucose monitoring. It measures the amount of glucose in a small blood sample and displays the results on its digital display. The device is compact and portable, allowing for convenient self-testing.
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5 protocols using accu chek compact plus glucometer
1
Streptozotocin-Induced Diabetes Model
2
Oral Glucose Tolerance Test in Rats
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Oral glucose tolerance test (OGTT) was conducted one week before the end of the study following an overnight fast. Fasting blood glucose was measured, and blood was collected for insulin determination. Rats then received an oral glucose gavage of 1 g/kg body weight and blood glucose values obtained at 10, 20, 30, 60, and 120 min using an Accu-Chek Compact Plus glucometer (Roche Diagnostics, Basel, Switzerland). Blood was collected at each time point during OGTT and centrifuged to obtain plasma for insulin analysis with ELISA (Alpco, Salem, NH, USA).
3
Glucose Tolerance Test in Diabetic Mice
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Homozygous db/db mice for spontaneous mutation (Leprdb) and heterozygous mice for control were fasted for 4 h, and then baseline glucose concentrations were assessed using an Accu-Chek Compact Plus glucometer (Roche Diagnostics, Indianapolis, IN) from tail blood. Mice were then injected (intraperitoneally, i.p) with 1 mg/g glucose. Blood glucose was determined at 15, 30, 60, 120, and 240 min following i.p. injection of glucose. Total serum fasting glucose from tail blood were also detected with a CardioChek PA system (PTS Diagnostics, Indianapolis, IN) under the manufacturer’s instructions. Detailed methodology was described as the previous protocol with a minor modification (Chen et al., 2015 (link); Zhang et al., 2017 (link)). Insulin level in serum was determined using a standard curve obtained from the standards provided by the kit. Serum insulin was assessed using a Triglyceride Colorimetric Assay Kit from Cayman Chemical (Ann Arbor, MI) according to the manufacturer’s instructions (Zhang et al., 2017 (link)).
4
Glucose Tolerance Test in Mice
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Oral glucose tolerance test was performed as described63 (link) following 7 days of antibody treatment in 9-week-old male C57BL/6 mice (n=6 per group) treated with either control antibody, a combination of REGN2477 and REGN1033 and ActRIIB.hFc at 10 mg kg−1 each. All groups were fasted overnight and the next morning given an oral gavage of 2 g kg−1 glucose at T0. Glucose was sampled by tail vein using an Accu-Chek Compact Plus Glucometer (Roche Diagnostics) at baseline, 15, 30, 60 and 120 min after administration.
5
Aminoguanidine Attenuates Diabetic Colonic Dysmotility
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Forty male Sprague-Dawley rats, weighing 150-180 g, were randomly assigned to four experimental groups, with 10 animals in each group, as follows: control group; control group treated with aminoguanidine (AG; Sigma, USA); diabetic group; and diabetic group treated with AG. Diabetes was induced by a single intraperitoneal (i.p.) injection of STZ at 60 mg/kg of body weight dissolved in a citrate buffer. Control rats received equal volumes of citrate buffer by i.p. injection. AG (1 g/L) was administered in drinking water from day 1. Diabetes was confirmed 1 week later by the measurement of tail vein blood glucose levels with an Accu-Chek Compact Plus Glucometer (Roche, USA). Only rats with final blood glucose levels >16.7 mmol/L were included in this study. At the end of week 16 following the administration of STZ, the animals were sacrificed after performing distal colonic transit test. The animal care, use, and experimental protocols were approved by the Institutional Animal and Use Committee of Nanjing Medical University (2016-SRFA-064).
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