Dehydroepiandrosterone dhea

Na₂HPO₄ and CaCl₂ • 2 H₂O were purchased from Riedel de Haen (Seelze, Germany). KH₂PO₄, CuSO₄ • 5 H₂O, H₃BO₃, potassium hydrogen phthalate, Na₂SO₄, nicotinic acid, MnSO₄ • H₂O, and KI were from Merck (Darmstadt, Germany). ZnSO₄ • 7 H₂O was purchased from Acros (Geel, Belgium). Tris, agar, NH₄HCO₃, NH₄Cl, FeCl₃ • 6 H₂O, MgCl₂ • 6 H₂O, glucose, Triton-X100, and biotin were from Roth (Karlsruhe, Germany). Inositol was from Th.Geyer (Berlin, Germany), and MoO₄ • 2 H₂O was purchased from Alfa Aesar (Kandel, Germany). Dehydroepiandrosterone (DHEA) was obtained from Steraloids (Newport, RI, United States). 4HP, ATP, and citric acid were purchased from Sigma Aldrich (Steinheim, Germany). 7-Hydroxycoumarin (7HC) and formic acid were purchased from TCI (Zwijindrecht, Belgium). ³⁴S labelled (NH₄)₂SO₄ and D₉-Salbutamol (D₉-SA) were purchased from Sigma-Aldrich (Taufkirchen, Germany), D₆-DHEA was obtained from Sigma-Aldrich (Saint Louis, MO, United States). Acetonitrile was from Fischer Scientific (Geel, Belgium), and HCOONH₄ was from VWR Chemicals (Damstadt, Germany). Ultrapure water was prepared with a Milli-Q water purification system LaboStar 2-DI/UV from SG Wasseraufbereitung und Regenerierstation GmbH (Barsbüttel, Germany). All other chemicals and reagents used were also of the highest grade available.

Testosterone, dehydroepiandrosterone (DHEA), and estradiol were purchased from Steraloids (Newport, RI). Triclocarban and triclosan were purchased from Sigma-Aldrich (St. Louis, MO). Triclocarban and triclosan were dissolved in DMSO for assay. JEG-3 cells were obtained from ATCC (Manassas, VA) for estradiol production assay because they contain CYP19A1.

Testosterone and dehydroepiandrosterone (DHEA) were purchased separately as 1 mg mL⁻¹ solutions in methanol from Steraloids Inc. (Newport, RI); d₃-Testosterone (ISTD for LC/MS) was purchased form Sigma Aldrich (Dorset, UK). Girard-T (GT) reagent, Dansyl Hydrazine (DS), α-cyano-4-hydroxycinnamic acid (CHCA), acetonitrile, methanol, acetone, ethanol, trifluoroacetic acid (TFA) and HPLC grade water were all obtained from Sigma Aldrich (Dorset, UK).