RNA was extracted using a spin column RNA isolation kit (Machery‐Nagel). On‐column DNase treatment was used to remove contaminating genomic DNA. Total RNA preparations were then used directly for qPCR (200 ng total RNA per reaction) using the Luna Universal One‐Step RT‐qPCR Kit (NEB, E3006) and target‐specific primer/probe sets (ThermoFisher) or frozen at −80°C until use. The qPCR primer/probe sets we used include the endogenous control Ipo8 (Mm01255158_m1), Vcam1 (Mm01320970_m1), Spp1 (Mm00436767_m1), Timp1 (Mm01341361_m1), and Col1a1 (Mm00801666_g1). The ∆∆Ct method was used to analyze the results.
Target specific primer probe sets
Manufactured by Thermo Fisher Scientific
Target-specific primer/probe sets are laboratory tools designed to amplify and detect specific DNA or RNA sequences. They consist of oligonucleotide primers and probes that are complementary to the target of interest. These sets enable researchers to selectively identify and quantify target molecules in complex samples using techniques such as real-time PCR or digital PCR.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using target specific primer probe sets
1
Quantitative Real-Time PCR Analysis
2
Quantitative gene expression analysis
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