Antibody to p21

TMOC was synthesized according to the previous report and was determined by spectra including ¹H-NMR, ¹³C-NMR, and high resolution mass spectrum (HRMS) which were great agreed with the literatures [14] (link), [15] (link). The purity of TMOC was more than 98% which was analyzed by HPLC.
RPMI-1640 medium and fetal bovine serum (FBS) were purchased from Thermo Scientific (South Logan, UT, USA). MTT, propidium indide (PI), 4,6-diamidino-2-phenylindole (DAPI), and the antibody to β-Actin were purchased from Sigma-Aldrich (St Louis, MO, USA). Gentian violet was purchased from Solarbio (Beijing, China). The Annexin V-FICT/PI apoptosis detection kit, invasion chambers, matrigel, and the antibody to p21 were purchased from BD Biosciences (Franklin Lakes, NY, USA). Cell lysis buffer and BCA protein assay kit were purchased from Beyotime (Shanghai, China). PVDF membrane and chemiluminescent reagents were from Millipore (Billerica, MA, USA). Antibodies to cyclin D1, CDK4, p16, p21, Bcl-xL, Bax, STAT3, p53, PTEN, c-myc were from Santa Cruz Biotechnology. Antibodies to phospho-Src (Tyr416), Src, phospho-STAT3 (Tyr705) and cleaved-PARP-1 were purchased from Cell Signaling Technology.

CPX (Sigma, St. Louis, MO, USA) was dissolved in ethanol to make the concentration of the stock solution of 1x10⁵μM. The final concentration of ethanol in the medium was no more than 0.02%, at which cell proliferation/growth or viability was not obviously altered. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and propidium iodide (PI) were purchased from Sigma. The Annexin V-PI kit was purchased from Keygen (Nanjing, China). Enhanced chemiluminescence (ECL) was purchased from Pierce (Rockford, IL, USA). Antibodies to ferritin, Bim, Mcl-1, cyclins A and D, Caspase-3, Rb, c-Myc, phospho-Rb, secondary antibodies of HRP-conjugated donkey anti-rabbit, and sheep anti-mouse were obtained from Cell Signaling Technology (Beverly, MA, USA). The antibody to p21 was purchased from BD Bioscience (San Jose, CA, USA), the antibody to β-catein was obtained from Millipore (Billerica, Massachusetts, USA), and the anti-GAPDH antibody was obtained from Kangchen Bio-Tech (Shanghai, China).