Human IL-18, IL-1β, and TNF-α were measured with the Human IL-18 Platinum ELISA, Human IL-1β ELISA and Human TNF-α ELISA Ready-SET-Go kits respectively (eBiosciences, San Diego CA) using 50 µL of plasma or 100 µL of culture supernatant. Each sample was tested in duplicate. Data was acquired using a SpectaMax M5 (Molecular Devices, Sunnyvale CA). The LLOD of IL-18 was 40 pg/mL in plasma and 18 pg/mL in culture supernatant.
Human il 1β elisa
Manufactured by Thermo Fisher Scientific
Sourced in United States, Austria
The Human IL-1β ELISA is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of human interleukin-1 beta (IL-1β) in various biological samples. It employs antibodies specific for IL-1β and uses the enzymatic color change reaction to detect and quantify the target analyte.
Automatically generated - may contain errors
Lab products found in correlation
Human il 18 platinum elisa, by Thermo Fisher Scientific (1 mentions)
Human tnfα elisa ready set go, by Thermo Fisher Scientific (1 mentions)
Spectamax m5, by Molecular Devices (1 mentions)
Dulbecco s modified eagle medium dmem, by Lonza (1 mentions)
Penicillin, by GE Healthcare (1 mentions)
Streptomycin, by GE Healthcare (1 mentions)
Amphotericin, by GE Healthcare (1 mentions)
Antibiotic antimycotic solution, by GE Healthcare (1 mentions)
Human il 6 elisa kit, by Thermo Fisher Scientific (1 mentions)
3 protocols using human il 1β elisa
1
Quantification of Inflammatory Cytokines
2
Cytokine Profiling of Vaccine Responses
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After treatment of the preterm, term, and adult blood with the described vaccines for 6 h, the plates were centrifuged (10 min, RT, 500 g), and supernatants collected and stored in three aliquots at −80°C for subsequent TNF (BD Biosciences Human TNF ELISA) and IL-1β ELISA (eBioscience Human IL-1β ELISA) and for subsequent multiplexing assays for Th1 (TNF, IL-1β, IL-12p70, IFNα, and IFNγ) and Th2 (IL-6, IL-10, and IL-12p40), and Th17 (IL-6, IL-1β) polarizing cytokines (Milliplex Human Magnetic Bead Panel; Millipore; Chicago, IL, USA). Data were analyzed on the Luminex® 100/200™ System using xPOTENT® software (Luminex; Austin, TX, USA).
3
Cultivation of Human Immune Cells
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McCoy’s 5A Modified Medium with L-Glutamine and Heat-Inactivated Fetal Bovine Serum (FBS) were purchased from American Type Culture Collection (Rockville, MD, USA). Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/l glucose and L-glutamine was from BioWhittaker (Verviers, Belgium). Antibiotic/Antimycotic Solution containing 10 000 U/ml penicillin, 10 mg/ml streptomycin, 25 µg/ml amphotericin and Lymphocyte Separation Medium (LSM) at a density of 1.077 g/ml were from PAA Laboratories GmbH (Pasching, Austria). Phosphate-Buffered Saline (PBS) was from Regional Blood Bank in Katowice (Katowice, Poland). 0.4% Trypan Blue Solution was from Sigma-Aldrich Chemical Corporation (Saint Louis, USA). Human IL-2 and IL-10 were from PeproTech Inc. (Rocky Hill, USA). Human IL-1β ELISA and Human IL-6 ELISA kits were from eBioscience (Vienna, Austria).
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