The 1H-NMR spectrum in CDCl3 (Deutero, Kastellaun, Germany) was recorded on a Bruker Avance 500 spectrometer (Bruker, BioSpin GmbH, Rheinstetten, Germany) equipped with a 5 mm BBO probe at 298 K. The chemical shifts were reported in parts per million (ppm) relative to TMS. The spectra were recorded with the standard 1H pulse program using 64 scans. All other NMR spectra were acquired on a Bruker Ascend 700 MHz NMR spectrometer at 298 K equipped with a 5 mm TXI cryoprobe. As a solvent, deuterated CD3OD was used. HSQC, HMBC, 1H-1H COSY spectra were recorded using the standard pulse programs from the TOPSPIN v. 3.6 software. Selective 1D TOCSY experiments were performed using mixing times of 120 ms.
Ascend 700 mhz nmr spectrometer
Manufactured by Bruker
Sourced in Germany
The Ascend 700 MHz NMR spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument designed for advanced spectroscopic analysis. It operates at a frequency of 700 MHz, providing a powerful platform for the investigation of molecular structures and dynamics.
Automatically generated - may contain errors
Lab products found in correlation
Avance 500 spectrometer, by Bruker (1 mentions)
Cdcl3, by Deutero (1 mentions)
Sephadex lh 20 column, by Merck Group (1 mentions)
5 mm txi cryoprobe, by Bruker (1 mentions)
Dionex ultimate 3000, by Thermo Fisher Scientific (1 mentions)
Nucleodur c18 htec column, by Macherey-Nagel (1 mentions)
Topspin 3, by Bruker (1 mentions)
Nmr suite 8, by Chenomx (1 mentions)
5 mm triple resonance probe, by Bruker (1 mentions)
4 protocols using ascend 700 mhz nmr spectrometer
1
NMR Spectroscopy of Organic Compounds
2
Characterization of Metabolites from Streptomyces albus
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S. albus ATGSal2P2::Tn14 was grown at 30 °C for 3 days in 6 × 500-mL flasks containing 50 mL of TSB, and pre-culture was used to inoculate 100 × 500-mL flasks containing 50 mL of NL19 media. Cultures were incubated at 30 °C for 5 days. Metabolites were extracted as described above. The extracts from biomass and the supernatant were combined and fractionated by size-exclusion chromatography on an LH 20 Sephadex column (Sigma-Aldrich, USA) using methanol as the solvent. The fractions were collected every 15 minutes., evaporated and dissolved in 0.5 mL of MeOH. Samples were further separated by preparative HPLC (Dionex UltiMate 3000, Thermo Fisher Scientific, USA) using a NUCLEODUR® C18 HTec column (250 × 10 mm, 5 µm) (Macherey-Nagel, Germany) with a linear gradient of solvent B (acetonitrile with 0.1% of formic acid) against solvent A (water with 0.1% of formic acid) at a flow rate of 4.5 mL/min at 45 °C. Compounds were separated using a gradient starting from 30% and increasing to 70% of B over 30 min. UV spectra were recorded with a DAD detector at 280 nm. Individual peaks were collected and analyzed by LC-MS as described above.
NMR spectra were acquired on a Bruker Ascend 700 MHz NMR spectrometer equipped with a 5 mm TXI cryoprobe (Bruker, USA). Deuterated CDCL3 was used as a solvent and HSQC, HMBC and 1H-1H COSY spectra were recorded using standard pulse programs (Table5S ).
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S. albus ATGSal2P2::Tn14 was grown at 30 °C for 3 days in 6 × 500-mL flasks containing 50 mL of TSB, and pre-culture was used to inoculate 100 × 500-mL flasks containing 50 mL of NL19 media. Cultures were incubated at 30 °C for 5 days. Metabolites were extracted as described above. The extracts from biomass and the supernatant were combined and fractionated by size-exclusion chromatography on an LH 20 Sephadex column (Sigma-Aldrich, USA) using methanol as the solvent. The fractions were collected every 15 minutes., evaporated and dissolved in 0.5 mL of MeOH. Samples were further separated by preparative HPLC (Dionex UltiMate 3000, Thermo Fisher Scientific, USA) using a NUCLEODUR® C18 HTec column (250 × 10 mm, 5 µm) (Macherey-Nagel, Germany) with a linear gradient of solvent B (acetonitrile with 0.1% of formic acid) against solvent A (water with 0.1% of formic acid) at a flow rate of 4.5 mL/min at 45 °C. Compounds were separated using a gradient starting from 30% and increasing to 70% of B over 30 min. UV spectra were recorded with a DAD detector at 280 nm. Individual peaks were collected and analyzed by LC-MS as described above.
NMR spectra were acquired on a Bruker Ascend 700 MHz NMR spectrometer equipped with a 5 mm TXI cryoprobe (Bruker, USA). Deuterated CDCL3 was used as a solvent and HSQC, HMBC and 1H-1H COSY spectra were recorded using standard pulse programs (Table
3
Polymer NMR Characterization Protocol
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NMR analysis was performed
with a Bruker Ascend 700 MHz NMR Spectrometer (Bruker, Germany) and
data were analyzed with the TopSpin 3.5 Software (Bruker, Germany).
NMR samples were prepared by dissolving 2–4 mg of polymer in
either 500 μL of CDCl3 (PEG derivates) or D2O (Alginate-CD). Spectra of the PEG derivatives were acquired at
299.7 K. For alginate samples, water suppression pulse sequence was
applied, and measurements were carried out at 325 K. Spectra were
calibrated with respect to nondeuterated solvent (CHCl3, 7.26 ppm or H2O, 4.29 ppm).
with a Bruker Ascend 700 MHz NMR Spectrometer (Bruker, Germany) and
data were analyzed with the TopSpin 3.5 Software (Bruker, Germany).
NMR samples were prepared by dissolving 2–4 mg of polymer in
either 500 μL of CDCl3 (PEG derivates) or D2O (Alginate-CD). Spectra of the PEG derivatives were acquired at
299.7 K. For alginate samples, water suppression pulse sequence was
applied, and measurements were carried out at 325 K. Spectra were
calibrated with respect to nondeuterated solvent (CHCl3, 7.26 ppm or H2O, 4.29 ppm).
4
Stingless Bee Honey Nutrient Analysis
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In this experiment, the nutritional content of the stingless bee honey sample used for the supplementation of mice was further analysed using the 1H NMR spectroscopy method as described by Ramli et al. [33 (link)]. Prior to testing, the water content for the honey sample was first measured using a hand-held refractometer. In this test, the 1H NMR spectra were recorded at 300 K on a Bruker Ascend 700 MHz NMR spectrometer (Bruker Biospin, Germany) equipped with a 5 mm triple-resonance probe (700.4 MHz 1H frequency). A total of 40 scans and four prior dummy scans of 65k points were acquired with a spectral width of 15.985 ppm, a receiver gain of 8, and an acquisition time of 1.46 s. The standard one-dimensional NOESY-presaturation pulse sequence suppressed the water resonance. All NMR spectra were manually phased, baseline-corrected, and calibrated by the trimethylsilylpropanoic acid (TSP) signal at 0.0 ppm using topsin 3.0. The NMR spectral data analysis was then performed using targeted profiling with Chenomx NMR Suite 8.1 software for metabolomics analysis. This analysis compares the integral of a known reference signal (TSP) with signals derived from a library of compounds containing chemical shifts and peak multiplicities.
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