Msd proinflammatory panel 1

Mouse hemibrain samples were assayed in duplicate using the MSD Proinflammatory Panel I (K15048D; MesoScale Discovery, Gaithersburg, MD, United States), a highly sensitive multiplex enzyme-linked immunosorbent assay (ELISA). This panel quantifies the following 10 proinflammatory cytokines in a single small sample volume (25 μL) of supernatant using an electrochemiluminescent detection method (MSD): interferon γ (IFN-γ), interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, and tumor necrosis factor α (TNFα). The mean intra-assay coefficient for each cytokine was less than 8.5%, based on the cytokine standards. Any value below the lower limit of detection (LLOD) for the cytokine assay was replaced with ½ LLOD of the assay for statistical analysis.

Mouse hemibrain samples were assayed in duplicate using the MSD Proinflammatory Panel I (K15048D; MesoScale Discovery, Gaithersburg, MD, United States), a highly sensitive multiplex enzyme-linked immunosorbent assay (ELISA). This panel quantifies the following 10 proinflammatory cytokines in a single small sample volume (25 μL) of supernatant using an electrochemiluminescent detection method (MSD): interferon γ (IFN-γ), interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, and tumor necrosis factor α (TNFα). The mean intra-assay coefficient for each cytokine was <8.5%, based on the cytokine standards. Any value below the lower limit of detection (LLOD) for the cytokine assay was replaced with 1/2 LLOD of the assay for statistical analysis.