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Digoxigenin labeled probes

Manufactured by Agilent Technologies

Digoxigenin-labeled probes are laboratory reagents used in various molecular biology techniques, such as in situ hybridization and Southern blotting. They facilitate the detection and localization of specific DNA or RNA sequences within biological samples. Digoxigenin is a hapten that can be attached to nucleic acid probes, allowing for their specific recognition and visualization through immunological detection methods.

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2 protocols using digoxigenin labeled probes

1

EBV, Kappa, and Lambda Detection

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Digoxigenin-labeled probes (DAKO) were used to detect EBV-encoded small RNA-1 and −2 (EBER-1/2) for EBV infection, immunoglobulin kappa light chain (IGK), and immunoglobulin lambda light chain (IGL). The assays were performed on a Leica Bond platform with prediluted probes for EBER, IGK, and IGL (Leica Biosystems).
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2

EBV and Immunoglobulin Light Chain Detection

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Digoxigenin-labeled probes (DAKO) were used to detect Epstein-Barr virus (EBV)-encoded small RNA-1 and -2 (EBER-1/2) for EBV infection, immunoglobulin kappa light chain (IGK), and immunoglobulin lambda light chain (IGL). The assays were performed on a Ventana Benchmark XT platform with an iViewBlue detection kit according to the manufacturer's manual (Ventana Medical Systems, Tucson, AZ, USA).
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