Imagem enhanced c9100 13

Manufactured by Hamamatsu Photonics

Sourced in Japan

The ImagEM Enhanced C9100-13 is a high-performance scientific EMCCD camera developed by Hamamatsu Photonics. It features a back-illuminated, electron-multiplying CCD image sensor designed for low-light imaging applications.

Automatically generated - may contain errors

Lab products found in correlation

Dsu spinning disc confocal microscope, by Olympus (1 mentions) Tcs sp5 2 confocal microscope, by Leica (1 mentions) Application suite, by Leica (1 mentions) Uplansapo objective, by Olympus (1 mentions) Volocity software, by PerkinElmer (1 mentions) Ultraview vox, by PerkinElmer (1 mentions) Plan apo 20 vc, by Nikon (1 mentions) Eclipse ti, by Nikon (1 mentions) Cfi plan apo vc 60x wi, by Nikon (1 mentions) Te2000, by Nikon (1 mentions)

4 protocols using imagem enhanced c9100 13

Confocal Microscopy Imaging of Midgut

Check if the same lab product or an alternative is used in the 5 most similar protocols

Images were obtained using a either a DSU spinning disc confocal
microscope (Olympus) equipped with UPLFLN ×20, ×40 oil
immersion and ×60 oil-immersion objectives and 512×512
EM-CCD camera (ImagEM Enhanced C9100-13, Hamamatsu Photonics) or a Leica
TCS SP5 II confocal microscope (Leica Microsystems) equipped with an
×40 oil-immersion and ×63 oil-immersion objective, 405
nm diode, 458, 476, 488, 496 and 514 nm Ar, 543 nm HeNe and 633 nm HeNe
lasers and digital zoom. Images were acquired using SlideBook (version
4.2, Intelligent Imaging Innovations) or Leica Application Suite (Leica
Microsystems) software.
For all images, Z-stacks through the midgut were acquired every
0.2–1 μm through the tissue.

Lucchetta E.M, & Ohlstein B. (2017). Amitosis of polyploid cells regenerates functional stem cells in the Drosophila intestine. Cell stem cell, 20(5), 609-620.e6.

+ Open protocol

+ Expand

Imaging Protrusions and Subcellular Localization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protrusions of cells expressing Lifeact-GFP (a gift from Roland Wedlich-Söldner, University of Munster, Munster, Germany; Riedl et al., 2008 (link)) in cell culture medium were observed with a spinning-disk confocal microscopy system (UltraVIEW VoX; PerkinElmer) with an inverted microscope (model IX81; Olympus), a 100× NA 1.40 oil immersion UPlanSApo objective (Olympus), and an electron multiplying charge-coupled device 14-bit 1K × 1K camera (ImagEM-1K C9100-50; Hamamatsu Photonics) at 37°C in 5% CO₂. A 488-nm laser was used for imaging. Images were acquired at an interval of 10 s for 5 min and then exported as TIFF files using Volocity software (version 6.0.1; PerkinElmer). All images were cropped and converted to AVI files using ImageJ (version 1.45f; National Institutes of Health). To observe localization of Bax, GFP-Bax, and DsRed-Mito were simultaneously visualized with the same microscope setup as that for Lifeact-GFP except that an electron multiplying charge-coupled device 16-bit 512 × 512 camera (ImagEM-Enhanced C9100-13; Hamamatsu Photonics) was used.

Yamauchi S., Hou Y.Y., Guo A.K., Hirata H., Nakajima W., Yip A.K., Yu C.H., Harada I., Chiam K.H., Sawada Y., Tanaka N, & Kawauchi K. (2014). p53-mediated activation of the mitochondrial protease HtrA2/Omi prevents cell invasion. The Journal of Cell Biology, 204(7), 1191-1207.

+ Open protocol

+ Expand

Fluorescence Imaging for Enzyme Kinetics

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescence images were obtained using an electron multiplying CCD camera (ImagEM Enhanced C9100-13, Hamamatsu Photonics K. K. (Japan)) and an inverted microscope (ECLIPSE Ti, Nikon Corp. (Japan)) equipped with 20× and 100× objective lenses (Plan Apo VC 20× (Nikon) and Plan Apo λ 100× oil (Nikon)). The 100× lens was used only for time-lapse imaging. Excitation and emission wavelengths were 470 nm and 535 ± 15 nm for fluorescein, 555 nm and 593 ± 20 nm for resorufin, and 395 nm and 480 ± 20 nm for 4-MU, respectively. For enzyme kinetic measurements and digital counting of enzyme, exposure time was 200 ms for fluorescein and 400 ms for resorufin. In dual-color enzyme assay, exposure time was 300 ms for fluorescein, 500 ms for resorufin and 100 ms for 4-MU. The fluorescence images were analyzed using ImageJ software (National Institutes of Health (USA)).

Ono T., Ichiki T, & Noji H. (2018). Digital enzyme assay using attoliter droplet array. The Analyst, 143(20), 4923-4929.

+ Open protocol

+ Expand

Imaging Mounted C. elegans Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols

We imaged mounted C. elegans early embryos on an inverted microscope (Nikon, TE2000) using a 60X water-immersion objective (Nikon, CFI Plan Apo VC 60X WI, NA 1.2). Images were acquired using a spinning disk confocal unit (Yokugawa, CSU-X1) equipped with continuous-wave lasers for fluorescence excitation and an EM-CCD camera (Hamamatsu, ImagEM Enhanced C9100-13) for detection. GFP fluorescence was excited at 488 nm and collected through a bandpass filter with 514-nm center and 30-nm full width at half maximum (FWHM) wavelength. mCherry fluorescence was excited at 561 nm and collected through a bandpass filter with 593-nm center and 40-nm FWHM wavelength. Fluorescent nanodiamonds (FNDs) were excited at 561nm and collected through a long-pass filter with 647-nm cut-on wavelength.
Figure S1A indicates the terminology for embryo orientation and axis labels used throughout this manuscript: The x-y plane is defined as the imaging plane. The longitudinal direction (or the long axis) of the oblong embryo is assigned as the x-direction, while the transverse direction refers to the y-direction.

Wu H., Kabacaoğlu G., Nazockdast E., Chang H., Shelley M., & Needleman D. (2021). Laser Ablation and Fluid Flows Show a Single Force Mechanism Governs Spindle Positioning.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!