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Dv897 emccd camera

Manufactured by Oxford Instruments
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The DV897) EMCCD camera is a high-performance imaging device designed for scientific and industrial applications. It utilizes Electron Multiplying Charge Coupled Device (EMCCD) technology to provide exceptional low-light sensitivity and high signal-to-noise ratio. The camera's core function is to capture and process digital images with high spatial and temporal resolution.

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Lab products found in correlation

Metamorph 7, by Molecular Devices (3 mentions) 89002 et ecfp eyfp filter, by Chroma Technology (3 mentions)

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EMCCD camera (203 citations)

3 protocols using dv897 emccd camera

1

Imaging of Protein Interactions via FRET

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Imaging used a Nikon TE2000-E microscope equipped with a 100X/1.4NA (Planapo) DIC oil immersion objective. Images were recorded with MetaMorph 7.1 software (Molecular Devices) on an ANDOR iXON (DV897) EMCCD camera with total magnification giving a pixel size of 106.7 nm, an analog gain of 4, and no electron multiplication. FRET and mECFP fluorescent images were obtained from a Dual-View™ 2 imaging device (Photometrics) with an 89002 ET-ECFP/EYFP filter (Chroma Technology Corp). This allowed simultaneous imaging of both the FRET channel (CFP excitation, YFP emission) and the mECFP channel (CFP excitation, YFP emission). 10-frame 3D stacks at 200 nm steps along the z-axis were collected at 60 sec intervals (FRET imaging and Stu2-tdTomato).
Suzuki A., Badger B.L., Haase J., Ohashi T., Erickson H.P., Salmon E.D, & Bloom K. (2016). How the kinetochore couples microtubule force and centromere stretch to move chromosomes. Nature cell biology, 18(4), 382-392.
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2

Imaging of Protein Interactions via FRET

Check if the same lab product or an alternative is used in the 5 most similar protocols
Imaging used a Nikon TE2000-E microscope equipped with a 100X/1.4NA (Planapo) DIC oil immersion objective. Images were recorded with MetaMorph 7.1 software (Molecular Devices) on an ANDOR iXON (DV897) EMCCD camera with total magnification giving a pixel size of 106.7 nm, an analog gain of 4, and no electron multiplication. FRET and mECFP fluorescent images were obtained from a Dual-View™ 2 imaging device (Photometrics) with an 89002 ET-ECFP/EYFP filter (Chroma Technology Corp). This allowed simultaneous imaging of both the FRET channel (CFP excitation, YFP emission) and the mECFP channel (CFP excitation, YFP emission). 10-frame 3D stacks at 200 nm steps along the z-axis were collected at 60 sec intervals (FRET imaging and Stu2-tdTomato).
Suzuki A., Badger B.L., Haase J., Ohashi T., Erickson H.P., Salmon E.D, & Bloom K. (2016). How the kinetochore couples microtubule force and centromere stretch to move chromosomes. Nature cell biology, 18(4), 382-392.
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3

Live-cell Imaging with FRET and ECFP

Cited 1 time
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Imaging was performed on a Nikon TE2000-E microscope equipped with a 100×/1.4NA (Planapo) DIC oil immersion objective. Images were recorded with MetaMorph 7.1 software (Molecular Devices) on an ANDOR iXON (DV897) EMCCD camera with a total magnification giving a pixel size of 106.7 nm with no electron multiplication. FRET and mECFP fluorescence images were obtained from a Dual-View 2 imaging device (Photometrics) with an 89002 ET-ECFP/EYFP filter (Chroma Technology). This allowed simultaneous imaging of both the FRET channel (CFP excitation, YFP emission) and the mECFP channel (CFP excitation, YFP emission). See details in [4 (link)].
Suzuki A., Gupta A., Long S.K., Evans R., Badger B.L., Salmon E.D., Biggins S, & Bloom K. (2018). A kinesin5, Cin8, recruits protein phosphatase 1 to kinetochores and regulates chromosome segregation. Current biology : CB, 28(17), 2697-2704.e3.
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