Gehc microview software

Manufactured by GE Healthcare

Sourced in United Kingdom

GEHC MicroView software is an imaging and analysis platform designed for the visualization and quantification of 3D data from micro-computed tomography (micro-CT) systems. It provides tools for image processing, segmentation, and analysis of small animal and sample data.

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Lab products found in correlation

Explore locus sp micro ct, by GE Healthcare (1 mentions) Ge explore locus sp micro ct, by GE Healthcare (1 mentions) Micro ct system, by GE Healthcare (1 mentions) μct100, by Scanco (1 mentions) Rs 9 micro ct scanner, by GE Healthcare (1 mentions)

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MicroView software (52 citations) MicroView (40 citations)

5 protocols using gehc microview software

Micro-CT Analysis of Scaffold-Guided Bone Regeneration

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Six months postsurgery, the 12 canines were euthanized with excessive anesthesia; mandibular specimens were then obtained and were immediately fixed in 10% formalin. The samples were scanned using a micro-CT device and reconstructed into 3D images to display gross morphology, as previously described [23 (link)]. The samples were scanned using a micro-CT system (GE eXplore Locus SP Micro-CT, USA). Scanning parameters were set at 80 kV and 80 μA, with an exposure time of 3000 ms and a pixel size of 15 μm. After scanning, 3D images were reconstructed using GEHC MicroView software (GE Healthcare Biosciences, Chalfont St. Giles, UK). To quantify the new bone formed around the scaffolds, a 20 mm × 10 mm region was analyzed for BMD, bone volume fraction (bone volume/total volume, BV/TV), TbTh, and trabecular number (TbN).

Yang C., Liu X., Zhao K., Zhu Y., Hu B., Zhou Y., Wang M., Wu Y., Zhang C., Xu J., Ning Y, & Zou D. (2019). miRNA-21 promotes osteogenesis via the PTEN/PI3K/Akt/HIF-1α pathway and enhances bone regeneration in critical size defects. Stem Cell Research & Therapy, 10, 65.

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Micro-CT Analysis of Bone Formation

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The fixed specimens were imaged and detected employing a GE eXplore Locus SP Micro-CT (GE Healthcare, Chicago, IL, USA) to visualize the new bone formed around the implants. The parameters were set at 80 μA current and 80 kV voltage, with an exposure time of 3,000 ms. The 3D images were reconstructed using GEHC MicroView software (GE Health-care, London, UK) after scanning. To obtain the parameters of bone mineral density and bone volume fraction (bone volume/total volume) of the newly formed bone around the implant, a region with a radius of 0.5 mm from the implant surface within the bone marrow cavity was chosen for analysis.

Zhang X., Yu Q., Wang Y.A, & Zhao J. (2018). Dose reduction of bone morphogenetic protein-2 for bone regeneration using a delivery system based on lyophilization with trehalose. International Journal of Nanomedicine, 13, 403-414.

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Micro-CT Analysis of Rat Skulls

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8 weeks after operation, all animals were euthanized and the specimens obtained were then fixed in 4% (w/v) paraformaldehyde. Specifically, the rat skulls were scanned using a micro-CT system (GE Healthcare Biosciences, Pittsburgh, USA) in high-resolution mode (voltage, 80 kV; current, 80 μA; pixel size, 15 μm). After scanning, the GEHC MicroView software (GE Healthcare Biosciences) was used to reconstruct 3D images of the rat skulls. The bone mineral density (BMD), bone volume/total volume (BV/TV), and trabecular thickness (Tb.Th) were analyzed for each group. Then, all specimens were decalcified, followed by wax immersion. Thereafter, hematoxylin and eosin (HE) staining and Masson staining of all serial sections were carried out.

Li J., Du H., Ji X., Chen Y., Li Y., Heng B.C, & Xu J. (2022). ETV2 promotes osteogenic differentiation of human dental pulp stem cells through the ERK/MAPK and PI3K-Akt signaling pathways. Stem Cell Research & Therapy, 13, 495.

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Microstructural Analysis of Implanted Scaffolds

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All specimens were scanned for vessels and bone formation within the defects after implant retrieval using a μCT imaging system (μCT 100, SCANCO Medical AG, Bassersdorf, Switzerland). The defect sites were scanned with a resolution of 10 μm, 70 kV voltage, 200 μA current, and 300 ms exposure time. After scanning, 3D images were reconstructed using GEHC Micro-View software (GE Healthcare BioSciences, Chalfont-St. Giles, UK). The scaffolds, bone, and blood vessels were separately reconstructed and displayed by extracting all voxels with different grey value ranges.

Li C., Jiang C., Deng Y., Li T., Li N., Peng M, & Wang J. (2017). RhBMP-2 loaded 3D-printed mesoporous silica/calcium phosphate cement porous scaffolds with enhanced vascularization and osteogenesis properties. Scientific Reports, 7, 41331.

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Micro-CT Imaging of Bone Density

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Computed tomography (CT) images were acquired as previously described (15 (link)). Briefly, images were acquired in 5 100-ms frames at 1° rotation increments with an X-ray tube potential of 80 kVp and a current of 450 µA using an RS-9 micro-CT scanner (GE Healthcare, London, Ontario, Canada). Two adjacent image volumes were acquired with an overlap of 40% of the field of view in the axial direction, and these images were stitched together after reconstruction. Images had a nominal isotropic resolution of 91 µm and required approximately 25 minutes of scan time. The bone was visualized using the GEHC Microview Software (GE Healthcare, London, ON, Canada). All image isosurfaces were visualized and analyzed for bone mineral density using the same image threshold value.

Dibra D., Mitra A., Xia X., Newman M., Cutrera J., Gagea M., Kleinerman E., Lozano G, & Li S. (2016). Lack of immunomodulatory Interleukin-27 enhances oncogenic properties of mutant p53 in vivo. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(15), 3876-3883.

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