The quantitative analysis of β-caryophyllene was conducted on an Agilent 1100 HPLC system with a UV/Vis detector. A reversed phase column (Kinetex, 5 μm EVO C18, 100 Å 150 × 3.0 mm, Phenomenex inc.) was installed. The mobile phases were DI H2O (solution A) and 90% acetonitrile (solution B). Both were acidified to 0.5% acetic acid and then filtered (0.22 μm) and degassed. β-Caryophyllene was analyzed in an isocratic mode with a flow rate of 0.5 mL min−1 and a column temperature of 40°C. A mobile phase ratio of 10:90 (A:B) was set and the samples were measured at 225 nm. The sample volume was set to 20 μL. A standard curve for β-caryophyllene from 0.01 to 1.2 g L−1 was determined beforehand. The evaluation was done on the ChemStation for LC 3D system software (Agilent Technologies).
Chemstation for lc 3d system software
Manufactured by Agilent Technologies
ChemStation for LC 3D system software is a data analysis and reporting software designed for use with Agilent's liquid chromatography (LC) instruments. It provides basic data processing and reporting capabilities for LC analyses.
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Lab products found in correlation
1100 hplc system, by Agilent Technologies (1 mentions)
Chlorophyll a, by Merck Group (1 mentions)
Chlorophyll b, by Merck Group (1 mentions)
Zeaxanthin, by Merck Group (1 mentions)
Agilent 1200 chemstation, by Agilent Technologies (1 mentions)
Lycopene, by Merck Group (1 mentions)
β carotene, by Merck Group (1 mentions)
Lutein, by Merck Group (1 mentions)
Violaxanthin, by CaroteNature (1 mentions)
Neoxanthin, by CaroteNature (1 mentions)
Antheraxanthin, by CaroteNature (1 mentions)
2 protocols using chemstation for lc 3d system software
1
Quantitative Analysis of β-Caryophyllene by HPLC
2
Carotenoid Quantification by HPLC
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Pigment content determinations were performed by HPLC as previously described (Fraser et al., 2000) (link) with modifications (D'Ambrosio et al., 2011) (link). Pigment separation was performed with an Agilent 1200 ChemStation HPLC system (Agilent Technologies, Inc., Santa Clara, USA) equipped with a DAD system using a C30 reverse-phase column (C30-YMC; 250 9 4.6 mm, S-5 lm, YMC, Milford, MA). Data acquisition and analysis were carried out with the ChemStation for LC 3D system software (Rev.B.03.02; Agilent Technologies, Santa Clara, CA). Quantification of carotenoids was carried out using calibration curves derived from analysis with authentic standards as previously described (Giorio et al., 2013) (link). Peak identification of violaxanthin, neoxanthin, antheraxanthin, lutein, zeaxanthin, β-carotene, Lycopene, chlorophyll a and chlorophyll b were based on retention times and spectral properties of authentic standards. Lycopene, β-carotene, lutein, zeaxanthin, chlorophyll a, chlorophyll b and 8ʹ-apo-β-carotenal were purchased from Sigma (Sigma-Aldrich, St. Louis, MO), violaxanthin, neoxanthin, and antheraxanthin were purchased from CaroteNature GmbH (Lupsingen, Switzerland).
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