SW620 human colon cancer cells from the American type culture collection (Rockville, MD, USA) were cultured in RPMI 1640 medium (Invitrogen Corp, Carlsbad, CA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS) (GIBCO BRL, Grand Island, NY, USA), 1 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a humidified atmosphere of 95% air and 5% CO2. As4O6 was obtained from Chonjisan institute (Seoul, Korea). Antibodies against procaspase 3, poly (ADP-ribose) polymerase (PARP), β-catenin, DR4, DR5, Bax, Bcl-2, Bid, cyclin B1, XIAP, p21, AKT 1/2/3 (H-136), phospho-Akt (Ser473), ERK, phospho-ERK (E-4), p53 and Beclin 1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against LC3, and Beclin-1, were purchased from PharMingen (San Diego, CA, U.S.A.). Antibodies against phospho-Akt (Thr 308), procaspase 8, procaspase 9, phospho-p38 MAPK, cdc2, JNK, and phospho-JNK were purchased from Cell signaling Technology, Inc. (Beverly, MA, USA). Antibody against β-actin was purchased from Sigma (Beverly, MA). Peroxidase-labeled donkey anti-rabbit and sheep anti-mouse immunoglobulin, and an enhanced chemiluminescence (ECL) kit were purchased from Amersham (Arlington Heights, IL, USA). All other chemicals not specifically cited here were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
Peroxidase labeled donkey anti rabbit and sheep anti mouse immunoglobulin
Manufactured by Cytiva
Sourced in United States
Peroxidase-labeled donkey anti-rabbit and sheep anti-mouse immunoglobulin is a laboratory reagent used in immunoassays and other immunochemical techniques. It consists of antibodies raised in donkeys against rabbit immunoglobulins, and antibodies raised in sheep against mouse immunoglobulins, which are conjugated with the enzyme peroxidase. This product is used to detect and quantify the presence of target proteins or other analytes in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence ecl kit, by Cytiva (7 mentions)
Antibody against β actin, by Merck Group (7 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Procaspase 3, by Santa Cruz Biotechnology (3 mentions)
β catenin, by Santa Cruz Biotechnology (2 mentions)
Fbs fetal bovine serum, by Thermo Fisher Scientific (2 mentions)
Icam 1, by Santa Cruz Biotechnology (2 mentions)
X linked iap xiap, by Santa Cruz Biotechnology (2 mentions)
Antibody against poly adp ribose polymerase parp, by BD (2 mentions)
Vascular endothelial growth factor (vegf), by Santa Cruz Biotechnology (2 mentions)
Mmp 2, by Santa Cruz Biotechnology (2 mentions)
Caspase activity assay kits, by R&D Systems (2 mentions)
C myc, by Santa Cruz Biotechnology (2 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
Beclin 1, by BD (1 mentions)
Procaspase 9, by Cell Signaling Technology (1 mentions)
Cyclin b1, by Santa Cruz Biotechnology (1 mentions)
Akt 1 2 3 h 136, by Santa Cruz Biotechnology (1 mentions)
Beclin 1, by Santa Cruz Biotechnology (1 mentions)
7 protocols using peroxidase labeled donkey anti rabbit and sheep anti mouse immunoglobulin
1
Apoptosis Induction in SW620 Colon Cancer Cells
2
MDA-MB-231 Cell Line Cultivation Protocol
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The triple-negative human breast cancer cell line MDA-MB-231 was obtained from Korea cell line bank and was sub-cultured with Roswell Park Memorial Institute Medium (RPMI) 1640 media (Hyclone, Marlborough, MA, USA) containing 10% of heat-inactivated (v/v) FBS (fetal bovine serum) (GIBCO BRL, Grand Island, NY, USA), 1 mM l-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified atmosphere of 95% air and 5% CO2. Antibodies against OCT-3/4, AKT, β-Catenin, ERK ½, ICAM-1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against CD44, E-cadherin, N-cadherin, were purchased from Abcam. Antibody against β-actin was purchased from Sigma (Beverly, MA, USA). Peroxidase-labeled donkey anti-rabbit and sheep anti-mouse immunoglobulin, and an enhanced chemiluminescence (ECL) kit were purchased from Amersham (Arlington Heights, IL, USA). All other chemicals not specifically cited here were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
3
Gastric Cancer Cell Line Cultivation
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Two of the gastric cancer cell lines, SNU-1, SNU-16 cells were obtained from the Laboratory of Cell Biology at the Cancer Research Institute in Seoul National University College of Medicine. They were cultured in RPMI-1640 supplemented with 10% fetal bovine serum (Gibco BRL, Carlsbad, CA, USA), 100 U of penicillin and 100 μg/mL of streptomycin at 37°C in the humidified atmosphere of 95% air and 5% CO2 in an incubator. Molecular mass markers for proteins were obtained from Pharmacia Biotech (Saclay, France). Antibodies against phospho-Akt (Ser473), Akt 1/2/3, X-linked inhibitor of apoptosis protein (XIAP), and procaspase 3 were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Antibodies against phospho-Akt (Thr 308), and phospho-p53 (Ser 15) were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Antibody against poly (adenosine diphosphate-ribose) polymerase (PARP) was purchased from BD Biosciences Pharmingen (San Diego, CA, USA). Antibody against β-actin was from Sigma (Beverly, MA, USA). Peroxidase-labeled donkey anti-rabbit and sheep anti-mouse immunoglobulin, and an enhanced chemiluminescence (ECL) kit were purchased from Amersham (Arlington Heights, IL, USA). All other chemicals not specifically cited here were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
4
AGS Cell Culture and Protein Analysis
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Human AGS cells from the American type culture collection (Rockville, MD, USA) were cultured in RPMI 1640 medium (Invitrogen Corp, Carlsbad, CA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS) (GIBCO BRL, Grand Island, NY, USA), 1 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a humidified atmosphere of 95% air and 5% CO2. Antibodies against Bcl-2, Bid, Bcl-xL, phospho p53, p53, BAX, c-IAP-2, X-linked IAP (XIAP), Akt, phospho Akt, ERK, phospho ERK, and procaspase 3 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against poly (ADP-ribose) polymerase (PARP) was purchased from PharMingen (San Diego, CA, USA). Antibody against β-actin was from Sigma (Beverly, MA, USA). Peroxidase-labeled donkey anti-rabbit and sheep anti- mouse immunoglobulin, and an enhanced chemiluminescence (ECL) kit were purchased from Amersham (Arlington Heights, IL). All other chemicals not specifically cited here were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
5
Anticancer Activity of Vitis coignetiae Pulliat
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Human lung cancer cell lines A549 cells from the American type culture collection (Rockville, MD, USA) were cultured in RPMI 1640 medium (Invitrogen Corp, Carlsbad, CA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS) (GIBCO BRL, Grand Island, NY, USA), 1 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a humidified atmosphere of 95% air and 5% CO2. Caspase activity assay kits were obtained from R and D Systems (Minneapolis, MN, USA). Antibodies against cyclin D1, c-Myc, MMP-2, ICAM-1, and VEGF were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against β-actin was from Sigma (Beverly, MA). Peroxidase-labeled donkey anti-rabbit and sheep anti-mouse immunoglobulin, and an enhanced chemiluminescence (ECL) kit were purchased from Amersham (Arlington Heights, IL). All other chemicals not specifically cited here were purchased from Sigma Chemical Co. (St. Louis, MO). The anthocyanins were isolated from Vitis coignetiae Pulliat.
6
Breast Cancer and Endothelial Cell Culture Using Anthocyanins
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The breast cancer cell line MCF-7 acquired from ATCC (Rockville, MD, USA) were subcultured with Roswell Park Memorial Institute Medium (RPMI) 1640 media (Hyclone, Marlborough, MA, USA) containing 10% of heat inactivated (v/v) FBS (fetal bovine serum) (GIBCO BRL, Grand Island, NY, USA), 1 mM l -glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified atmosphere of 95% air and 5% CO2. Human umbilical vein endothelial cells (EA.hy 926 cells, ECs) were obtained from ATCC and cultured in medium 199 (GIBCO BRL, Grand Island, NY, USA) supplemented with 20% FBS, 2 mM l -glutamine, 5 U/mL heparin, 100 IU/mL penicillin, 10 μg/mL streptomycin, and 50 μg/mL EC growth supplements. Antibodies against COX-2, MMP-2, MMP-9, VEGF, β-Catenin, Cyclin D1, C-myc, ICAM, E-cadherin, Survivin, IkB, p-IkB, and NF-κB were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against β-actin was from Sigma (Beverly, MA, USA). Peroxidase-labeled donkey anti-rabbit and sheep anti-mouse immunoglobulin, and an enhanced chemiluminescence (ECL) kit were purchased from Amersham (Arlington Heights, IL, USA). All other chemicals not specifically cited here were purchased from Sigma Chemical Co. (St. Louis, MO, USA). We used the anthocyanins isolated from Vitis coignetiae Pulliat [8 (link)].
7
Apoptosis Regulation in U937 Cells
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U937 human leukemic cells from the American type culture collection (Rockville, MD, USA) were cultured in RPMI 1640 medium (Invitrogen Corp, Carlsbad, CA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS) (GIBCO BRL, Grand Island, NY, USA), 1 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37℃ in a humidified atmosphere of 95% air and 5% CO2. Antibodies against Bcl-2, Bid, inhibitors of apoptosis protein-1 (IAP-1), IAP-2, X-linked IAP (XIAP), pro-caspase 3, pro-caspase 8, and pro-caspase 9 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against poly (ADP-ribose) polymerase (PARP) was purchased from PharMingen (San Diego, CA, USA). Antibody against β-actin was from Sigma (Beverly, MA). Peroxidase-labeled donkey anti-rabbit and sheep anti-mouse immunoglobulin, and an enhanced chemiluminescence (ECL) kit were purchased from Amersham (Arlington Heights, IL). Caspase activity assay kits were purchased from R&D systems (Minneapolis, MN, USA). All other chemicals not specifically cited here were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
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