Dylight 488 secondary antibody

Manufactured by Thermo Fisher Scientific

Sourced in United States

The DyLight 488 secondary antibody is a fluorescent labeling agent used in various immunodetection techniques. It is designed to bind to primary antibodies and emit green fluorescence when excited with the appropriate wavelength of light, enabling the visualization and detection of target proteins or antigens in biological samples.

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Lab products found in correlation

Confocal laser scanning microscope, by Olympus (1 mentions) A1r mp, by Nikon (1 mentions) Cm1850 uv cryostat, by Leica (1 mentions)

Close spelling variants of this product

DyLight 488 (158 citations) DyLight 488-conjugated secondary antibodies (17 citations) DyLight 488- or 594-conjugated secondary antibody (4 citations) DyLight 488-labeled secondary antibody (2 citations) Goat anti-rabbit IgG DyLight 488 conjugated secondary antibody (2 citations) Goat-anti-rabbit DyLight 488 secondary antibody (2 citations) DyLight 488 fluorescence-labeled secondary goat anti-mouse antibody (2 citations)

4 protocols using dylight 488 secondary antibody

Quantifying Endogenous IAA in Torpedo Embryos

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To estimate the concentration of endogenous IAA, samples were immediately ground in liquid nitrogen and extracted in 1 mL 80% cold methanol, which contains 10 ng/mL ²H₅‐IAA (Olchemlm, Olomouc, Czech Republic), as an internal standard. Further extraction and quantitative analyses of IAA were performed as described previously (Liu et al., 2012). The experiments were conducted with three biological replicates.
Samples of torpedo embryos were fixed in 50% FAA as described previously (Hou and Huang, 2005). Sections were incubated with anti‐rabbit Dylight 488 secondary antibody (Thermo Scientific, Waltham, MA) for immunofluorescence. Fluorescence was assayed using a confocal laser‐scanning microscope (Olympus, Tokyo, Japan).

Xu J., Yang X., Li B., Chen L., Min L, & Zhang X. (2018). GhL1L1 affects cell fate specification by regulating GhPIN1‐mediated auxin distribution. Plant Biotechnology Journal, 17(1), 63-74.

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Immunofluorescence Staining of PDLSCs

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To verify the results of western blotting, we plated the PDLSCs in 12-well plates (2 × 10⁴/well) to perform immunofluorescence staining. After 1 day or 3 days of treatment, PDLSCs were fixed with 4% (v/v) paraformaldehyde, followed by incubation with 0.5% Triton X-100 and 5% goat serum. Related primary antibodies (1:200–1:300; anti-TNF-α, anti-IL-1β, anti-IL6, anti-OPN, and anti-Runx2) were incubated with the samples overnight. After that, the cells were incubated with DyLight 488 secondary antibody (1:400; Thermo Fisher Scientific, MA, USA) to conjugate with fluorescence before staining with FITC-labeled phalloidin and DAPI. Subsequently, cell morphology and the intensity of protein fluorescence were observed using a confocal laser microscope (A1R MP⁺, Nikon, Japan).

Zhou M., Gao S., Zhang X., Zhang T., Zhang T., Tian T., Li S., Lin Y, & Cai X. (2020). The protective effect of tetrahedral framework nucleic acids on periodontium under inflammatory conditions. Bioactive Materials, 6(6), 1676-1688.

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Immunohistochemical Analysis of GM2 Ganglioside

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For immunohistochemical analysis, mice were anesthetized and transcardiac perfusion was initiated with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde in PBS. Brain tissues were removed and placed in the same fixative overnight at 4 °C, and then treated sequentially with 10%, 20% and 30% sucrose in PBS overnight at 4 °C. Brains were embedded in OCT (Sakura) and kept at − 80 °C until used. Ten micrometer sections were taken by Leica Cryostat (CM1850-UV) at − 20 °C and the G_M2 ganglioside was immunostained as previously described by KM966 primer and DyLight 488 secondary antibody (Thermo) [9] (link). Slides were studied on fluorescent Microscopy (Olympus BX53).

Timur Z.K., Akyildiz Demir S., Marsching C., Sandhoff R, & Seyrantepe V. (2015). Neuraminidase-1 contributes significantly to the degradation of neuronal B-series gangliosides but not to the bypass of the catabolic block in Tay–Sachs mouse models. Molecular Genetics and Metabolism Reports, 4, 72-82.

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Multimodal Imaging of GLP1R in Islets

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GLP1R^SNAP/SNAP islets were incubated for 30 min with 100 nM LUXendin551/LUXendin645 and 500 nM BG-TMR/BG-JF₅₄₉/BG-SulfoCy5 in culture medium. For immunostaining, islets were fixed in 4% formaldehyde fixation for 12–15 min before application of mouse monoclonal anti-GLP1R antibody (Iowa DHSB; mAb #7F38, 1:30) overnight at 4 °C, diluted in PBS + 0.1% Triton + 1% BSA. Goat anti-mouse DyLight488 secondary antibody (ThermoFisher Scientific; Cat #35502, 1:1000) was applied for 1 h at room temperature followed by mounting with Vectashield Hardset containing DAPI. Imaging was performed identically to above, with excitation and detection wavelengths for LUXendin645 – 633 nm / 641–694 nm; DyLight488 – 488 nm / 490–561 nm; DAPI – 405 nm / 410–498 nm. If required, Gaussian denoising was performed. LUXendins are available from Celtarys Research.

Ast J., Nasteska D., Fine N.H., Nieves D.J., Koszegi Z., Lanoiselée Y., Cuozzo F., Viloria K., Bacon A., Luu N.T., Newsome P.N., Calebiro D., Owen D.M., Broichhagen J, & Hodson D.J. (2023). Revealing the tissue-level complexity of endogenous glucagon-like peptide-1 receptor expression and signaling. Nature Communications, 14, 301.

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