Methanol, acetic acid, and acetonitrile were purchased from Mallinckrodt (Phillipsburg, NJ). The peptide AADAADAA was custom synthesized by NeoBioLab (Cambridge, MA). The (−)-(18-crown-6)-2,3,11,12-tetracarboxylic acid and ethylenediaminetetraacetic acid tetrasodium salt dihydrate were obtained from Sigma Aldrich (St. Louis, MO). The bis(sulfosuccinimidyl) suberate (BS3) cross-linker was obtained from Thermo Fisher Scientific Inc.(Rockford, IL). The N-hydroxysuccinimide ester of 4-trimethylammonium butyrate (TMAB-NHS) reagent was a generous donation from Prof. Fred Regnier. All peptide solutions for positive nanoelectrospray (nESI) were prepared in 49.5/49.5/1 (v/v/v) Methanol/water/acetic acid, while the peptide solutions for negative nESI were mixed in 49.5/49.5/1 (v/v/v) Methanol/water/ammonium hydroxide solution (~50 μM). The BS3, ethylenediaminetetraacetic acid (EDTA) and (−)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18C6-TCBA) reagents were dissolved in water (~2 mM), while the TMAB-NHS was dissolved in acetonitrile (~2 mM).
Ethylenediaminetetraacetic acid tetrasodium salt dihydrate
Manufactured by Merck Group
Sourced in United States
Ethylenediaminetetraacetic acid tetrasodium salt dihydrate is a chemical compound that functions as a chelating agent. It is commonly used in various laboratory applications to bind and sequester metal ions.
Automatically generated - may contain errors
Lab products found in correlation
Milli q water purification system, by Merck Group (2 mentions)
Acetone, by Merck Group (2 mentions)
Protease inhibitor cocktail, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Bis sulfosuccinimidyl suberate bs3 cross linker, by Thermo Fisher Scientific (1 mentions)
Methanol, by Mallinckrodt (1 mentions)
Acetonitrile, by Mallinckrodt (1 mentions)
Acetic acid, by Mallinckrodt (1 mentions)
Triethylammonium bicarbonate, by Merck Group (1 mentions)
Sucrose, by Thermo Fisher Scientific (1 mentions)
Formaldehyde ch2o, by Merck Group (1 mentions)
Tris hcl, by Merck Group (1 mentions)
N octyl β d glucopyranoside, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Trisodium phosphate, by Merck Group (1 mentions)
Diethylenetriaminepentaacetic acid, by Merck Group (1 mentions)
Meso 2 3 dimercaptosuccinic acid, by Merck Group (1 mentions)
6 protocols using ethylenediaminetetraacetic acid tetrasodium salt dihydrate
1
Peptide Crosslinking and Characterization
2
Protein Extraction and Proteomic Analysis
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Acetonitrile (ACN), methanol (MeOH), acetic acid (HAc), formic acid (FA), ammonium bicarbonate (NH4HCO3), tri-n-butylphosphate (TBP), sodium chloride (NaCl) were obtained from Merck (Darmstadt, Germany). Acetone, ethylenediaminetetraacetic acid tetrasodium salt dihydrate (EDTA), protease inhibitor cocktail, phosphate buffered saline (PBS), Tris-HCl, diethylamide (DEA), trifluoroacetic acid (TFA), n-octyl-β-D-glucopyranoside, triethyl ammonium bicarbonate (TEAB), and formaldehyde CH2O (37% (vol/vol)) were purchased from Sigma Aldrich (St. Louis, MO, USA). For tryptic digestion, iodoacetamide (IAA), urea and dithiothreitol (DTT) were obtained from Sigma Aldrich and trypsin (sequencing grade from bovine pancreas 1418475; Roche diagnostic, Basel, Switzerland) were used. Formaldehyde (13CD2O) (20% (vol/vol), 99% 13C, 98% D) and sodium cyanoborodeuteride (NaBD3CN) (96% D) were purchased from Isotec (Miamisburg, OH). Sodium cyanoborohydride (NaBH3CN) was obtained from Fluka (Buchs, Switzerland). Sucrose was purchased from Fisher Scientific Company (Göteborg, Sweden). Triton X-114 was obtained from KEBO Lab (Stockholm, Sweden). Ultrapure water was prepared by Milli-Q water purification system (Millipore, Bedford, MA, USA).
3
Chelator-Impregnated Silica Supports
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In the present work, chelators in a completely basic form (all acidic functional groups were titrated with Na+) were impregnated into the macroporous silica support using their aqueous solutions (typically, 0.25–2.0 wt% chelator concentrations). For the preparation of the aqueous solutions, trisodiumphosphate (1 in Fig. 3a , Sigma Aldrich, 96%) and ethylenediaminetetraacetic acid tetrasodium salt dihydrate (4 , Sigma Aldrich, 99%) were directly dissolved in deionized water. In the cases of chelators in an acidic form, such as 1-hydroxyethane 1,1-diphosphonic acid monohydrate (2 , Sigma Aldrich, 95%), ethylenediamine tetramethylene phosphonic acid (3 , Tokyo Chemical Industry, 98%), diethylenetriaminepentaacetic acid (5 , Sigma Aldrich, 99%), and meso-2,3-dimercaptosuccinic acid (6 , Sigma Aldrich, 98%), controlled amounts of NaOH were dissolved together in deionized water to completely titrate acidic H+ into Na+. After the wet impregnation of the chelators into the silica support, the samples were dried in a vacuum oven at 100 °C for 12 h. The final loading of chelators was controlled in the range of 0–4 wt% with respect to the mass of silica support.
4
Heavy Metal Analysis in Environmental Samples
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The aluminum cans were obtained from commercial shops (Mansoura, Egypt). Isatoic anhydride, dibenzoylmethane, 4-amino-2-hydroxy benzoic acid, toluene, cadmium(ii ) chloride, chloroacetyl chloride, nickel(ii ) chloride hexahydrate, ethylenediaminetetraacetic acid tetrasodium salt dihydrate, zinc(ii ) chloride, aluminum(iii ) chloride hexahydrate, iron(iii ) chloride hexahydrate, and lead(ii ) chloride were obtained from Sigma-Aldrich. Hydrochloric acid, potassium chloride, sodium hydroxide, mercury(ii ) chloride, and copper(ii ) chloride dihydrate were purchased from Merck (Germany). The other chemicals were of analytical grade, obtained from Fluka (Switzerland) and were used without further purification unless otherwise stated. In addition, all aqueous solutions in this work were prepared by using doubly distilled water (resistance: 18.2 MΩ at 25 °C). It is worth mentioning that all glassware was rinsed in concentrated nitric acid solution (1% (v/v)) and washed with doubly distilled water before use. For analytical applications, the cucumber, lettuce leaves, and wastewater samples were collected from different agricultural locations in Samanoud (El-Gharbia).
5
Comprehensive Protein Extraction and Digestion
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Acetonitrile (ACN), methanol (MeOH), acetic acid (HAc), formic acid (FA), ammonium bicarbonate (NH4HCO3) were obtained from Merck (Darmstadt, Germany). Acetone, ethylenediaminetetraacetic acid tetrasodium salt dihydrate (EDTA), protease inhibitor cocktail, phosphate buffered saline (PBS), trifluoroacetic acid (TFA), triethyl ammonium bicarbonate (TEAB), sodium citratemonobasic, sodium dodecyl sulphate (SDS), and chicken ovalbumin were purchased from Sigma Aldrich (St. Louis, MO, USA). For tryptic digestion, iodoacetamide (IAA), urea and dithiothreitol (DTT) were obtained from Sigma Aldrich and trypsin/Lys-C mixture (mass spectrometry grade; Promega, Mannheim, Germany). Ultrapure water was prepared by Milli-Q water purification system (Millipore, Bedford, MA, USA).
6
Metabolic Assays for Insulin Sensitivity
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Acetic acid, propionic acid and butyric acid were purchased from Sigma-Aldrich (Saint Louis, MO, USA) as well as bovine serum albumin fatty-acid free (BSA), human insulin solution, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), Triton X-100 (TX-100), β-nicotinamide adenine dinucleotide reduced disodium salt hydrate (NADH), sodium pyruvate, β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), sulfosalicylic acid solution (SSA), EthylenediaminetetraAcetic acid tetrasodium salt dihydrate (EDTA), reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione reductase (GR), 2-vinylpyridine (2-VP), 5,5ʹ-Dithio-bis-(2-nitrobenzoic Acid) (DTNB), Potassium dihydrogen phosphate (KH2PO4), Dipotassium hydrogen phosphate (K2HPO4), human insulin solution, metformin, and menadione. Ethanol and the Pierce bicinchoninic acid (BCA) protein assay kit were purchased from ThermoFisher Scientific (Fremont, CA, USA). The Glucose Uptake-Glo assay kit was purchased from Promega (Leiden, The Netherlands).
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