A1rsi confocal fluorescence microscope

Manufactured by Nikon

Sourced in Japan

The A1Rsi confocal fluorescence microscope is a high-performance imaging system designed for advanced fluorescence microscopy. It features a high-resolution confocal scanning system that enables detailed imaging of fluorescently labeled samples. The microscope provides precise control over the excitation and detection of fluorescent signals, allowing for efficient and accurate data acquisition.

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Lab products found in correlation

Monoclonal anti α tubulin antibody, by Merck Group (1 mentions) Ax70 fluorescence microscope, by Olympus (1 mentions) Goat anti mouse igm, by Thermo Fisher Scientific (1 mentions) Rat anti gfp antibody, by Nacalai Tesque (1 mentions) Mab1326, by Abcam (1 mentions) Confocal fluorescence microscope, by Leica (1 mentions) Brn3a, by Santa Cruz Biotechnology (1 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions)

Close spelling variants of this product

A1Rsi confocal microscope Confocal fluorescence microscope A1Rsi microscope A1Rsi Fluorescence microscope Confocal microscope

3 protocols using a1rsi confocal fluorescence microscope

Immunostaining and Imaging of Oligodendrocytes

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The cultured cells were fixed with 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer (pH 7.4) and used for immunostaining. Fixed cells were blocked with 5% normal goat serum in phosphate-buffered saline and 0.1% Triton X-100 (PBST) and then incubated with primary antibodies overnight at 4°C. The primary antibodies used were as follows: rat anti-GFP antibody (1/500, Nacalai Tesque, 04404-84), monoclonal anti-α-tubulin antibody (1/500, Sigma, T9026), rat anti-myelin basic protein (MBP) antibody (Millipore, MAB386), monoclonal O4 antibody (1/300, R&D systems, MAB1326) and rabbit anti-paxillin antibody (1/250, abcam, ab32084, clone Y113). After being rinsed with PBST, the cells were incubated with secondary antibodies. The secondary antibodies used were Alexa 488- or 594-conjugated goat anti-mouse, anti-rabbit and anti-rat IgG or goat anti-mouse IgM (Molecular Probes). Fluorescent signals were visualized using AX70 fluorescence microscope (Olympus, Tokyo) and A1Rsi confocal fluorescence microscope (Nikon, Tokyo). The number of OL primary processes was analyzed using “Analyze/Sholl” tool of Fiji software based on ImageJ (NIH).

Shimizu T., Murakoshi H., Matsumoto H., Ichino K., Hattori A., Ueno S., Ishida A., Tajiri N, & Hida H. (2021). Tension Sensor Based on Fluorescence Resonance Energy Transfer Reveals Fiber Diameter-Dependent Mechanical Factors During Myelination. Frontiers in Cellular Neuroscience, 15, 685044.

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Multiplex Immunofluorescence Staining

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Formalin-fixed paraffin-embedded tissue sections were subjected to pretreatment involving antigen retrieval by heating in EDTA buffer at the beginning of the experiment and to a TR1 retrieval between each staining. Tissue sections were then stained for Cyclin D1, IL32, CD68 and CD3 with polymer enhancer and HRP 2-Step polymer and the buffer 1X Plus Amplification Diluent with Opal 570, 650, 520 and 690 for the detection of Cyclin D1, IL32, CD68 and CD3, respectively. We used DAPI (1:4000) to stain the nuclei. The experiment was conducted in an automated lmpath36. Images were acquired on a Nikon A1 RSi confocal fluorescence microscope with spectral module. Additional methods are detailed in the Online Supplementary Methods section and Online Supplementary Table S2.

Decombis S., Papin A., Bellanger C., Sortais C., Dousset C., Le Bris Y., Riveron T., Blandin S., Hulin P., Tessoulin B., Rouel M., Le Gouill S., Moreau-Aubry A., Pellat-Deceunynck C, & Chiron D. (2022). The IL32/BAFF axis supports prosurvival dialogs in the lymphoma ecosystem and is disrupted by NIK inhibition. Haematologica, 107(12), 2905-2917.

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Immunofluorescence Analysis of Retinal and Optic Nerve Cells

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Rat eyes were fixed in 4% paraformaldehyde overnight at 4 °C, cryoprotected by immersion in 30% sucrose for at least 2 h, and then embedded in optimal cutting temperature compound. Frozen retinal cross-sections were cut at a thickness of 12 μm iPSC-derived RGC cells and human optic nerve head astrocytes were fixed for 15 min using 4% paraformaldehyde. Sections or cells were incubated with blocking buffer (3% bovine serum albumin, 4% normal goat serum, 0.1% Triton 100-X in 1x phosphate buffered saline) for 1 h at room temperature and incubated with primary polyclonal Brn3a (Santa Cruz, TX, 1:200), GFAP (Sigma-Aldrich, MO, 1:500), ASMase (ASM H181, Santa Cruz, 1:200) or NSMase (nSMase 2, ECM bioscience, 1:100) antibody overnight at 4 °C. Goat anti-rabbit Alexa 488 or 594 secondary antibody (Molecular Probes) was used at a dilution of 1:500. Propidium iodide at a dilution of 1:3000 was used to visualize nuclei. Retinal sections were imaged using a Leica confocal fluorescence microscope with a 20x objective lens. iPSC-derived RGCs and human optic nerve head astrocytes were imaged using a Nikon A1Rsi confocal fluorescence microscope with 20x and 60x objective lenses.

Hopfner K.P., Karcher A., Shin D., Fairley C., Tainer J.A, & Carney J.P. (2000). Mre11 and Rad50 from Pyrococcus furiosus: Cloning and Biochemical Characterization Reveal an Evolutionarily Conserved Multiprotein Machine. Journal of Bacteriology, 182(21), 6036-6041.

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