Mouse igg1 isotype control

For flank tumors, intra-tumoral injections started when KP1 ART1^OE tumors became palpable on day 11 and every 72 hours until day 23 after tumor inoculation. Mice were injected with 5mg/kg ART1 antibody 22C12 for group ‘22C12’ or Mouse IgG1 isotype control (BioXcell, Cat# BP0297) for group ‘Iso Ctrl’. Tumor sizes were measured every 72 hours and mice were euthanized on day 25 after tumor inoculation, when tumors were weighed and processed for flow cytometry staining. For the orthotopic lung tumor models, mice were injected intravenously with 0.5×10⁵ KP1 ART1^OE cells on day 0. Where indicated, mice were i.p. injected with 25 mg/kg ART1 antibody 22C12 (22C12) (used in in vivo studies reported in Fig. 3A to F, 3I to K, 4A to C, S4B to H, and fig. S5A to F, S8B, S8E to F). The more recently developed fully humanized 22C12 antibody (22C12 (HuLC)) was used to treat mice i.p. (25 mg/kg) in in vivo studies assessing absolute immune cell counts in tumor-bearing lungs (reported in Fig. 3G and H and fig. S4C, D, G, and H). As controls, mice were treated with 25 mg/kg of Mouse IgG1 isotype control (BioXcell, Cat# BP0297) for group ‘Iso Ctrl’. i.p. injections started from day 6 and continued every 72 hours until day 18 as indicated.