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Aperio imagescope application

Manufactured by Leica
Sourced in United States

Aperio ImageScope is a digital pathology software application that allows users to view and analyze whole slide images captured by Leica's high-performance digital slide scanners. It provides a user-friendly interface for accessing, navigating, and interacting with digital slide images.

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4 protocols using aperio imagescope application

1

Histological Analysis of Anorectal and Bladder Tissues

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Following euthanasia and perfusion, a necropsy was performed. Bladders and anorectal specimens were dissected from the lambs and stored in 10% buffered formalin. The following specimens were obtained: a 1-cm portion of the anus, a 2-cm portion of the rectum three centimeters from the anal verge, and a 1-cm portion of the bladder detrusor muscle between the ureters. Specimens were dehydrated in 30% sucrose solution and cryopreserved in Optimal Cutting Temperature (OCT) compound. The anus specimens were sectioned radially; the rectum and bladder specimens were sectioned transversely. All histologic sections were cut at 11 μm thickness, stained with hematoxylin and eosin (H&E) and coverslipped. The resulting H&E slides were digitally scanned at 20x magnification on the Aperio AT2 scanner. Average thicknesses of the anal sphincter complex, longitudinal and circular rectal muscles, and the detrusor muscles were measured using the ruler tool on the Aperio ImageScope application (Leica Biosystems).
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2

Histological Analysis of Anorectal and Bladder Tissues

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Following euthanasia and perfusion, a necropsy was performed. Bladders and anorectal specimens were dissected from the lambs and stored in 10% buffered formalin. The following specimens were obtained: a 1-cm portion of the anus, a 2-cm portion of the rectum three centimeters from the anal verge, and a 1-cm portion of the bladder detrusor muscle between the ureters. Specimens were dehydrated in 30% sucrose solution and cryopreserved in Optimal Cutting Temperature (OCT) compound. The anus specimens were sectioned radially; the rectum and bladder specimens were sectioned transversely. All histologic sections were cut at 11 μm thickness, stained with hematoxylin and eosin (H&E) and coverslipped. The resulting H&E slides were digitally scanned at 20x magnification on the Aperio AT2 scanner. Average thicknesses of the anal sphincter complex, longitudinal and circular rectal muscles, and the detrusor muscles were measured using the ruler tool on the Aperio ImageScope application (Leica Biosystems).
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3

High-Resolution Whole Slide Imaging

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Slides were scanned at a 40X magnification using a single z-plane via an Aperio AT2 whole slide scanner (Leica Biosystems, San Diego, CA, USA). Scanned digital WSI were evaluated for quality and to ensure that they were in focus. De-identified digital files in (.svs) format were stored on an image server for remote evaluation using the Aperio ImageScope application (Leica Biosystems, Buffalo Grove, IL, USA).
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4

High-Resolution Whole Slide Imaging

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Slides were scanned at a 40X magnification using a single z-plane via an Aperio AT2 whole slide scanner (Leica Biosystems, San Diego, CA, USA). Scanned digital WSI were evaluated for quality and to ensure that they were in focus. De-identified digital files in (.svs) format were stored on an image server for remote evaluation using the Aperio ImageScope application (Leica Biosystems, Buffalo Grove, IL, USA).
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