Mmp12

Manufactured by Proteintech

Sourced in United States, China

MMP12 is a recombinant protein produced by Proteintech. It is a member of the matrix metalloproteinase (MMP) family, which are enzymes involved in the breakdown of the extracellular matrix. MMP12 has a catalytic domain that enables it to cleave various extracellular matrix components.

Automatically generated - may contain errors

Lab products found in correlation

Mmp 9, by Abcam (2 mentions) Alexa fluor 488, by Abcam (1 mentions) Cxcl1, by Proteintech (1 mentions) β catenin, by Abcam (1 mentions) Lsm 880 confocal microscope, by Zeiss (1 mentions) Hsp70, by Santa Cruz Biotechnology (1 mentions) Adiponectin, by Proteintech (1 mentions) β actin, by Proteintech (1 mentions) Enzyme labelling instrument, by Thermo Fisher Scientific (1 mentions) Ripa lysis buffer, by Wuhan Servicebio Technology (1 mentions) β actin, by Affinity Biosciences (1 mentions) Ripa buffer, by Keygen Biotech (1 mentions) Immobilon ecl system, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Phospho stat3, by Cell Signaling Technology (1 mentions) Gapdh, by Proteintech (1 mentions) Citrate buffer ph 6, by Thermo Fisher Scientific (1 mentions) 3 amino 9 ethylcarbazole aec, by Vector Laboratories (1 mentions) Ihc kit, by Enzo Life Sciences (1 mentions)

5 protocols using mmp12

Immunohistochemical Analysis of Hair Follicle Markers

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The samples were fixed in 4% paraformaldehyde, dehydrated, then embedded in paraffin and sectioned (4–6 μm).
All procedures were performed according to the standard protocol. Antibodies and dilutions used were: CXCL1 (1:100, proteintech), MMP12 (1:50, proteintech), SPP1 (1:50, Abcam), EGR1 (1:50, Abcam). β-catenin (1:250,Abcam), BMP2 (1:100, Abcam), FGF5 (1:100, Abcam), ki67 (1:250,Abcam), Secondary antibodies: Alexa Fluor-488 (Abcam). Nuclei were stained using 4 diamidino-2-phenylindole (DAPI). Proliferation and apoptosis of hair follicles were assessed by Ki67/TUNEL immunostaining as reported before (Foitzik et al., 2006 (link)). Imaging was performed using Zeiss LSM 880 confocal microscope (Zeiss, Oberkochen, Germany).

Chen J., Fan Z.X., Zhu D.C., Guo Y.L., Ye K., Dai D., Guo Z., Hu Z.Q., Miao Y, & Qu Q. (2021). Emerging Role of Dermal White Adipose Tissue in Modulating Hair Follicle Development During Aging. Frontiers in Cell and Developmental Biology, 9, 728188.

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Western Blot Protein Analysis

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Western blotting was performed as described previously.4 Samples of equivalent total protein (20 μg) were loaded. Primary antibodies against CD63, CD81, HSP70 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), β‐Actin, ZIP4, Adiponectin, MMP9 and MMP12 (ProteinTech Group, Rosemont, IL, USA) were used.

Jin H., Liu P., Wu Y., Meng X., Wu M., Han J, & Tan X. (2018). Exosomal zinc transporter ZIP4 promotes cancer growth and is a novel diagnostic biomarker for pancreatic cancer. Cancer Science, 109(9), 2946-2956.

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Colon Tissue Protein Extraction and Analysis

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Proteins were extracted from the colon tissues using RIPA Lysis Buffer (Wuhan Servicebio Technology Co., Ltd, China) and the protein concentration was determined by bicinchoninic acid (BAC) method on the enzyme labelling instrument (Thermo Fisher Scientific, USA). The SDS-PAGE was used to separate the protein, and subsequently transferred to polyvinylidene fluoride (PVDF) membranes. The membranes were first blocked for 1h with 5% BSA at room temperature, and then probed with primary antibodies of MMP1 (Affinity, dilution of 1:1000), MMP3 (Abcam, dilution of 1:5000), MMP7 (Abcam, dilution of 1:1000), MMP9 (Abcam, dilution of 1:1000), MMP12 (Proteintech, dilution of 1:1000) and β-Actin (Affinity, dilution of 1:5000) at 4°C for overnight and then incubated with HPR-conjugated antibody at 37°C for one hour. Finally, the protein bands were recorded by ELC (Thermo Fish Scientific, USA) and quantified by Image J software to evaluate the protein relative expressions compare with β-Actin.

Chen W., He L., Zhong L., Sun J., Zhang L., Wei D, & Wu C. (2021). Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification. Drug Design, Development and Therapy, 15, 4125-4140.

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Protein Expression Analysis via Western Blot

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Total protein was extracted from cells using RIPA buffer (KeyGEN, China) supplemented with a protease inhibitor cocktail (CWBIO CW2200, China) and phosphatase inhibitor (CWBIO CW2383, China). Equal amounts (20 μg-40 µg) of protein were separated via SDS‒PAGE and transferred onto PVDF membranes (Millipore, Billerica, MA). After being blocked for 1 h in 5% milk, the membranes were incubated with antibodies targeting GAPDH (Proteintech, China), MMP9 (Abcam), MMP12 (Proteintech), STAT3 (Cell Signaling Technology), and phospho-STAT3 (Cell Signaling Technology) at 4°C overnight. After being incubated with secondary antibodies (Jackson ImmunoResearch, USA) for 1 h at room temperature, the protein bands were measured with the Immobilon ECL system (Millipore, S.p.A., Italy). ImageJ software was used for densitometric analyses.

Liu T., Zhang Z., Shen W., Wu Y, & Bian T. (2023). MicroRNA Let-7 Induces M2 Macrophage Polarization in COPD Emphysema Through the IL-6/STAT3 Pathway. International Journal of Chronic Obstructive Pulmonary Disease, 18, 575-591.

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Immunohistochemical Analysis of Aortic Markers

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Paraffin-embedded and formalin-fixed 5-μm aortic sections were subjected to heat-induced antigen retrieval with citrate buffer, pH6 (Thermo Scientific, MA). The slides were incubated overnight at 4 °C with mouse-specific primary antibodies for anti - CD163 cat # PA5–78961 Invitrogen, MMP-12 Cat# 50–173-3976 ProteinTech, and IFN-γ Cat# NBP2–66900 Novus Biologics. The sections were incubated with relevant secondary antibodies (Goat anti- Mouse) Invitrogen. Immunohistochemistry (IHC) staining was completed with IHC kit (Enzo Life Sciences, NY). Slides were visualized by 3-Amino-9-ethylcarbazole (AEC) (Vector Laboratories, Burlingame, CA) chromogens followed by an appropriate counterstain.

Dhital S., Rice C.D, & Vyavahare N.R. (2021). Reversal of elastase-induced abdominal aortic aneurysm following the delivery of nanoparticle-based pentagalloyl glucose (PGG) is associated with reduced inflammatory and immune markers. European journal of pharmacology, 910, 174487.

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