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4 protocols using sodium chlorite

1

Kraft Lignin Oxidation Protocol

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Kraft lignin (low sulfonate content, Mw of ∼10000), acetone (99.5%), methanol
(99.9%), glacial acetic acid (100%), sodium hypochlorite (NaClO, 6–14%
active chlorine), TEMPO (99%), and potassium hydroxide (KOH, pellets,
≥85%) were purchased from Sigma-Aldrich, Sweden AB. 95% sulfuric
acid and sodium hydroxide (NaOH, pure pellets) were purchased from
Merck KGaA, Germany. Sodium chlorite (NaClO2, high purity,
chlorite content of 77.5%–82.5%), a 0.1 M standardized NaOH
solution, and a 0.5 M standardized hydrochloric acid (HCl) solution
were purchased from VWR, Sweden. All chemicals were used without any
further purification.
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2

Oxidative Delignification of Birch Wood

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2,2,6,6-Tetramethyl-1-piperidinyloxy (TEMPO),
sodium bromide (NaBr), 1-cyano-2-ethoxy-2-oxoethylidenaminooxy)dimethylaminomorpholinocarbenium
hexafluorophosphate (COMU), and trifluoroethylamine hydrochloride
(TFEA·HCl) were purchased from Sigma-Aldrich, Sweden, and used
as received. Sodium hydroxide (NaOH), sodium hypochlorite (NaOCl),
sodium acetate trihydrate, sodium chlorite, ethanol, and acetonitrile
were purchased from VWR, Sweden. Deionized water was used from a laboratory
setup. The birch wood (Betula pendula) was purchased from Fredricsons Trä, Sweden.
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3

Bioactive Fruit Extraction and Analysis

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The açai, noni, and araza fruits were obtained from Quibdó (Chocó, Colombia—(5°41′32″ N 76°39′29″ W)). The reagents used were 99.8% ethanol (Mallinckrodt, Dublin 15, Ireland), n-hexane (Panreac, Darmstadt, Germany), sulfuric acid (Loba Chemie, Mumbai, India, acetic acid (Loba Chemie, Mumbai, India), sodium chlorite (JT-Baker, Deventer, Netherlands), hydrochloric acid 37% (Panreac, Darmstadt, Germany) and sodium hydroxide (Mol Lab, Bogotá, Colombia).
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4

Enzymatic Delignification of Lignocellulosic Biomass

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Cellulase enzyme (Trichoderma reesei (ATCC 26921)) was purchased from Sigma Aldrich (St Louis, MO, USA). Sodium sulfide (>60%) (Na2S), sodium hydroxide (min 98%) (NaOH), ethanol (95%) (C2H5OH), anhydrous calcium chloride (>74%) (CaCl2), hydrofluoric acid (55%) (HF), hydrochloric acid (37%) (HCl), and toluene (min 99%) (C7H8) were obtained from Chemiz (Shah Alam, Malaysia), whereas glacial acetic acid (99.8%) (CH3COOH) was obtained from Ajax Chemicals (Melbourne, Australia), and sodium chlorite (>79%) (NaClO) was obtained from JT Baker (Radnor, PA, USA). Sulfuric acid (96%) (H2SO4) was obtained from Merck (Darmstadt, Germany).
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