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Pgc1α

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PGC1α is a protein that functions as a transcriptional coactivator. It is involved in the regulation of genes that control energy metabolism and mitochondrial biogenesis.

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Lab products found in correlation

Phos s6 ribosomal protein, by Cell Signaling Technology (2 mentions) S6 ribosomal protein, by Cell Signaling Technology (2 mentions) Phos 4ebp1, by Cell Signaling Technology (2 mentions) Lamin a c, by Cell Signaling Technology (2 mentions) 4e bp1, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Phosmapk, by Cell Signaling Technology (1 mentions) Rhodamine conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Phos erk, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Cy3 conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)

2 protocols using pgc1α

1

Antibody Characterization for Cell Biology

Cited 1 time
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The antibodies used for this study were AMYLASE (A8273; Sigma-Aldrich), Atg5 (PM050, MBL, Woburn, MA), phos-TFEB (ABE1971; Millipore, Burlington, MA), TFEB (A303-673A; Bethyl Laboratories, Montgomery, TX), CK19 (TROMA-III, Developmental Studies Hybridoma Bank, Iowa City, IA), GAPDH (2118; Cell Signaling Technology, Danvers, MA), p62 (H00008878-M01; Abnova, Taipei, Taiwan), phos-4EBP1 (9451; Cell Signaling Technology), 4EBP1 (9452; Cell Signaling Technology), phos-MAPK (9101; Cell Signaling Technology), MAPK (9102; Cell Signaling Technology), phos-S6-ribosomal protein (4858; Cell Signaling Technology), S6-ribosomal protein (2217; Cell Signaling Technology), GFP (sc-9996; Santa Cruz Biotechnology, Dallas, TX), LAMIN A/C (2032; Cell Signaling Technology), LAMP1 (1D4B and H4A3; Developmental Studies Hybridoma Bank, Iowa City, IA), LAMP2 (ABL-93 and H4A4; Developmental Studies Hybridoma Bank), and PGC1α (PAB12061; Abnova). The TAP antibody was generated by Thomas Kolodecik from Yale University.45 (link) Antibodies for VATP6V1a and VATP6V1b2 are gifts from Dr. Dennis Brown from Harvard Medical School. The Anti-LC3 antibody was generated as previously described.43 (link) HRP-conjugated, FITC-conjugated and Rhodamine-conjugated secondary antibodies were from Jackson ImmunoResearch (West Grove, PA).
Wang S., Ni H.M., Chao X., Ma X., Kolodecik T., De Lisle R., Ballabio A., Pacher P, & Ding W.X. (2020). Critical Role of TFEB-Mediated Lysosomal Biogenesis in Alcohol-Induced Pancreatitis in Mice and Humans. Cellular and Molecular Gastroenterology and Hepatology, 10(1), 59-81.
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2

Antibody Panel for Western Blot Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for western blot analysis: TFEB (Bethyl, A303-673A), β-Actin (Sigma, A5441), GAPDH (Cell Signaling, 2118), p62 (Abnova, H00008878-M01), GFP (Santa Cruz), phos-s6-ribosomal protein (Cell Signaling, 4858), phos-4EBP1 (Cell Signaling, 9451), s6-ribosomal protein (Cell Signaling, 2217), 4EBP1 (Cell Signaling, 9452), phos-ERK (Cell Signaling, 9101), ERK (Cell Signaling, 9102), anti-lymphocyte antigen B superfamily (Ly6B, AbD Serotec, MCA771G), Lamin A/C (Cell Signaling, 2032), Lamp1 (DSHB, 1D4B), TFE3 (Sigma, HPA023881), PGC1α (Abnova, PAB12061), mTOR (Cell Signaling, 2972). Antibodies for ATPase H+ Transporting V1 Subunit 1A (VATP6V1a) and ATPase H+ Transporting V1 Subunit 1B2 (VATP6V1b2) are gifts from Dr. Dennis Brown from Harvard Medical School. The Anti-LC3 antibody was generated as previously described5 (link). HRP-conjugated, FITC-conjugated and Cy3-conjugated secondary antibodies were from Jackson ImmunoResearch.
Chao X., Wang S., Zhao K., Li Y., Williams J.A., Li T., Chavan H., Krishnamurthy P., He X.C., Li L., Ballabio A., Ni H.M, & Ding W.X. (2018). Impaired TFEB-mediated Lysosome Biogenesis and Autophagy Promote Chronic Ethanol-induced Liver Injury and Steatosis in Mice. Gastroenterology, 155(3), 865-879.e12.
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