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Auto gamma system arc 7010b

Manufactured by Hitachi
Sourced in Japan

The Hitachi Auto Gamma System ARC-7010B is a laboratory instrument designed for the measurement and analysis of radioactive samples. It provides automated gamma-ray spectroscopy capabilities for a wide range of applications.

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2 protocols using auto gamma system arc 7010b

1

Radiolabeled Compound Characterization Protocol

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Commercial reagents and solvents were purchased from Sigma-Aldrich (St. Louis, MO, USA), Wako Pure Chemical Industries (Osaka, Japan), Nacalai Tesque, Inc., (Kyoto, Japan), Tokyo Chemical Industry, Co., Ltd., (Tokyo, Japan), and Macrocyclics (Dallas, TX, USA). All of these were used without further purification unless otherwise stated.
[67Ga]GaCl3 and [123I]BMIPP were supplied by Nihon Medi-Physics Co., Ltd. (Tokyo, Japan). The radioactivity was measured by an Auto Gamma System ARC-7010B (Hitachi, Ltd., Tokyo, Japan).
Proton nuclear magnetic resonance (1H-NMR) spectra was recorded on JEOL JNM-ECS400 (JEOL Ltd, Tokyo, Japan). Figures of the NMR spectra are available in the Supplementary Material file (S6 Fig). Electrospray ionization mass spectra (ESI-MS) were obtained with JEOL JMS-T100TD (JEOL Ltd). TLC analysis was performed using silica plates (Art 5553, Merck, Darmstadt, Germany).
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2

Analytical Techniques for Chemical Characterization

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Auto Gamma System ARC-7010B (Hitachi, Ltd., Tokyo, Japan) recorded radioactivity. Thin-layer chromatography (TLC) on silica plates 60 F254 (Merck, Darmstadt, Germany) was used to monitor the reactions. High-performance liquid chromatography (HPLC) of SPD-20A system (Shimadzu Corp., Kyoto, Japan), using a Cosmosil® 5SL-II (20 ID × 250 mm) column (Nacalai Tesque, Inc., Kyoto, Japan). Nuclear magnetic resonance (NMR) spectroscopy was conveyed on JNM-ECS 400 and JNM-ECA 600 (JEOL Ltd., Tokyo, Japan). Direct analysis in real-time mass spectrometry (DART-MS) and electrospray ionization mass spectrometry (ESI-MS) were conducted on JMS-T100TD (JEOL Ltd., Tokyo, Japan). The optical density in 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium monosodium salt (WST-8) assay was conducted on Infinite® F200 Pro microplate reader (TECAN, Männedorf, Switzerland).
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