Microparticles were prepared from different PPDL-PTHF materials, which are multiblock copolymers synthesized from precursors of oligo(ω-pentadecalactone) and oligotetrahydrofuran, as well as from PPDL-PCL and PCL-PCL multiblock copolymers comprising oligo(ε-caprolactone) segments (for synthesis and characterization, see Supplementary Materials ). Particle preparation involved to dissolve 40 or 80 mg of the respective polymer in 1 mL of methylene chloride, dispersing the polymer solution by vortexing it (2500 rpm; MS1, IKA, Staufen, Germany) in a 2 mL aqueous solution of 2 wt.% polyvinyl alcohol (PVA; Mowiol® 4-88; Kuraray, Frankfurt a.M., Germany), and adding it to a hardening bath (0.5 wt.% Mowiol® 4-88) for solvent extraction/evaporation with magnetic stirring for 3 h. The microparticles were collected by centrifugation and lyophilized at 0.080 mbar (Alpha 1-2LD plus, Christ, Osterode, Germany). Analysis by static light scattering (Mastersizer 2000, Malvern, Herrenberg, Germany) using the Fraunhofer approximation showed average particle sizes of 30–50 µm.
Mowiol 4 88
Manufactured by Kuraray
Sourced in Germany
Mowiol® 4-88 is a polyvinyl alcohol (PVOH) product manufactured by Kuraray. It is a water-soluble, odorless, and colorless polymer. Mowiol® 4-88 is characterized by a degree of hydrolysis of 88% and a viscosity of 3-5 mPa·s in a 4% aqueous solution at 20°C.
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3 protocols using mowiol 4 88
1
Multiblock Copolymer Microparticle Synthesis
2
Curcumin-Loaded PLGA Nanoparticles with Chitosan Coating
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PLGA (Resomer RG503H) was purchased from Evonik Industries AG (Essen, Germany). CUR was obtained from Sigma Aldrich (Steinheim, Germany). Chitosan hydrochloride was supplied by Heppe Medical Chitosan GmbH (Halle/Saale, Germany). Polyvinyl alcohol (PVA, Mowiol 4-88) was provided by Kuraray (Hattersheim, Germany). Acetonitrile (ACN, HPLC gradient grade) was purchased from Fisher Scientific UK Ltd (Loughborough, UK). Water used was Millipore Q-Gard 2 by the water purification system of Merck Millipore (MA, USA).
Nanoparticles were prepared according to a previously reported modified solvent-displacement method [Citation23]. Batch sizes of 500 mg PLGA were dissolved in acetonitrile under vigorous stirring at room temperature for 10 min. Then, 500 μl of a 1% (m/V) solution of CUR in acetonitrile was added. The total prepared amount of 30-ml polymer-drug solution was injected into 200 ml stirring aqueous phase containing 2% PVA using a syringe pump with a flow rate of 0.5 ml/min. The dispersion was stirred overnight for solvent evaporation and then purified three times by centrifugation at 10,000 RCF for 20 min. For positively charged chitosan-coated nanoparticles, chitosan hydrochloride was dissolved in the aqueous phase in addition to PVA.
Nanoparticles were prepared according to a previously reported modified solvent-displacement method [Citation23]. Batch sizes of 500 mg PLGA were dissolved in acetonitrile under vigorous stirring at room temperature for 10 min. Then, 500 μl of a 1% (m/V) solution of CUR in acetonitrile was added. The total prepared amount of 30-ml polymer-drug solution was injected into 200 ml stirring aqueous phase containing 2% PVA using a syringe pump with a flow rate of 0.5 ml/min. The dispersion was stirred overnight for solvent evaporation and then purified three times by centrifugation at 10,000 RCF for 20 min. For positively charged chitosan-coated nanoparticles, chitosan hydrochloride was dissolved in the aqueous phase in addition to PVA.
3
Perfluorodecalin and PLGA Synthesis
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Cis-, trans-Perfluorodecalin 95% was ordered from Merck KGaA/Sigma-Aldrich (Darmstadt, Germany). PLGA was obtained as Resomer RG 503 H from Evonik Industries (Darmstadt, Germany). Polyvinyl alcohol (PVA), Type: Mowiol 4-88, was obtained from Kuraray Co., Ltd. (Tokyo, Japan).
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