The apoptosis of PTX-resistant SKOV3 and HeyA8 cells was determined using Annexin V-fluorescein isothiocyanate (Annexin V-FITC)/propidium iodide (PI) detection kit (Yeasen Biotech, Shanghai, China). In short, at 48 h after transfection, cells were collected via centrifuging. Cells were mixed with 100 μL binding buffer (Yeasen Biotech). After that, Annexin V-FITC (Yeasen Biotech) and PI (Yeasen Biotech) were employed to dye the cells. Finally, these samples were assessed using flow cytometry (BD Biosciences, San Diego, CA, USA).
100 μl binding buffer
Manufactured by Yeasen
The 100 μL binding buffer is a laboratory reagent designed for use in various biotechnological applications. It serves as a solution for binding and facilitating the interaction between molecules during experimental procedures. The buffer's core function is to provide the necessary chemical environment for efficient binding processes, without further interpretation of its intended use.
Automatically generated - may contain errors
2 protocols using 100 μl binding buffer
1
Apoptosis Assay for PTX-Resistant Cells
2
Apoptosis Detection by Annexin V-FITC/PI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This assay was employed to detect cell apoptosis with Annexin V-fluorescein isothiocyanate (Annexin V-FITC)/PI apoptosis detection kit (Yeasen) as per guidebook. Briefly, the transfected cells were harvested and then suspended in 100 μL binding buffer (Yeasen), followed by the incubation with Annexin V-FITC (Yeasen) and PI (Yeasen) at room temperature for 12 min in dark. Cell apoptotic rate was revealed after samples were assessed with a flow cytometry (BD Biosciences).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!