Wild type (wt) (pGL3-wt-JMJD2C-3’untranslated region [UTR]) or mutant (mut) (pGL3-mut-JMJD2C-3’UTR) reporter plasmids (GenePharma, Shanghai, China) were co-transfected with agomir NC or miR-216b agomir into MG63 and SaOS-2 cells. Following a 48 h period of transfection, the cells were collected and lysed. The subsequent procedures were performed with using a luciferase assay kit (K801–200, Biovision, Milpitas, CA, USA) following the manufacturer’s instructions on the luciferase reporter assay system (Promega, Madison, WI, USA). The relative luciferase activity was calculated based on the ratio of the luciferase activity of firefly luciferase to that of renilla luciferase. The sequences of wt-JMJD2C-3’UTR and mut-JMJD2C-3’UTR were 5′-GCAUGUAUGCUAAUGAGAUUU-3′ and 5′-GCUGUAAACGACGUCUCUAAA-3′, respectively [30 (link)].
Pgl3 mut jmjd2c 3 utr
Manufactured by GenePharma
Sourced in China
The PGL3-mut-JMJD2C-3'UTR is a genetic engineering product designed for experimental research purposes. It serves as a tool for studying the expression and regulation of the JMJD2C gene, which is involved in various biological processes. The specific function and intended use of this product should be determined by the researcher based on their experimental needs and protocols.
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2 protocols using pgl3 mut jmjd2c 3 utr
1
JMJD2C 3'UTR Luciferase Reporter Assay
2
Luciferase assay for JMJD2C 3'UTR
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Wild type (wt) (pGL3-wt-JMJD2C-3'untranslated region [UTR]) or mutant (mut) (pGL3-mut-JMJD2C-3'UTR) reporter plasmids (GenePharma, Shanghai, China) were co-transfected with agomir NC or miR-216b agomir into MG63 and SaOS-2 cells. Following a 48 h period of transfection, the cells were collected and lysed. The subsequent procedures were performed with using a luciferase assay kit (K801-200, Biovision, Milpitas, CA, USA) following the manufacturer's instructions on the luciferase reporter assay system (Promega, Madison, WI, USA). The relative luciferase activity was calculated based on the ratio of the luciferase activity of firefly luciferase to that of renilla luciferase. The sequences of wt-JMJD2C-3'UTR and mut-JMJD2C-3'UTR were 5′-GCAUGU AUGCUAAUGAGAUUU-3′ and 5′-GCUGUAAACG ACGUCUCUAAA-3′, respectively [30] .
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